Effects or*rhrptiic inhalzatiLn or ciclarette
smok-L on tht,
frequnn.cy or puimonj:y_~umors in mtce
Cecile Leucht-2nburour, Ph.D. (Professor)
Hend, Departriunt u~ C-ytochumistry,
and Rudolf Leuchtznburgur, ff.. D. (Prufessor)
Heads Depnrtry-L2nt or Cytochomistry
Swiss Institute for Experimental Cancer Research,
Laut--,-one, Switzerland.
This report.de3ls with results obtained by the au
th ur s
during the last 10 'years in chronic inhalation
akperinunts with
cigarette smoke carried out on CF- and on Snell's
mice, using
two different model systems. 1 -
1. Chronic exposure or qrouva of CF mice to
contiruous
aged whole cigarette smoke in a smoke filled
chamber.
2. Chronic exposure of individual Snellts mice in
rontainers
to pufra of -fr*esh whole cigarette smoke or ite
gas phase,
alt,3rnating witi, rrosh air, imitating as closely
as possible the
human habit or cigarette smoking.
Chronic exposure of cr mice to aged whole
cigaretto smukr.,
I
did not show a stati sLiC311Y sign if icant
increase or pul.-norizziry
tumors, when compared with cant rals, in mice at
19-2P months (,f
bus. However, a soriewhat, earlirzr appearanco of
pulmonaLy tumozs,
was noted in the experimental gruups.
Chronic axposure of male and fFimale Snell's mice
to puffs or
fresh wholu cig-irette smake or to the gas phase
resulted in a
significant incrs~jse in frequency of mice with
pulmuniry tum(irs,
especiully.of adenocarcincincs, when comparud with
co-itrols, where-
by the effect was more pronouncod after exposure
to the gas ph-se
than brter exposure to whole smoke. Comparison of
frequencies
between males and females ruv-,i.L- a greater
increase of adeno-
carcinomas in males than in females, while
fainales showud a great~er
increase of adanomns th.;n the males, after
inhalation of ciqzirutte
smuku. While the data suggcot that pulmonary
carcinogenE33is in
Snell's mice is enhanced after inholation,of whole
fresh cigazette
smoke or its gas phase, the differences in
frequency betweer. tu-nc,r
in males and females support the. concept that
horman-il faLtors
are alqo involved.
In cr Imine, pulmonary tumors were observed at
ages less titan.
12 months, while in Snallta micu, pulmonary tumors
were observed
in controls onlv efLar In months or agog nnd in
exposed mico after
15 Months of zg~. Histologically the pulmonary
tumors were adenomas,
and adendcarcinomas. No ca-.u of bronchugenic
spidermuid luita cance-
was found either in CF or Snell's controls, or in
cr l ar Snel L's.
mice exposed to chroniA inhalation of ciger.ette
$M'ake.
L
Cecile Lauchten'borger, Ph.D. Rudoli
Louchtenberger, M. D*i
Head, Uspeftment or CytuchemL&tr-, Head,
Depirtment of Cytoch3mittry*
BATCo document for Province of British Columbia 4
November 1999