25 th Juns, 1956
Pr. Wrx- & SMOKE M-P PWXRAWR
of I-
The present prograsome of the looks Analysis Orcup
consists
1) Physical measurements of Cigarette and Smoke
Tmoperatures
2) quantitative Analysis of neutral, non-volatile
(hTdrocarboz)
fraction for 3s4A*nzpyrsn*
This progre-9 may be ectended in two voyst
a) Qualitative Au&17sis of wake directed towards
discovering
the nature and structure of components in make.
b) Quantitative Analysis of smoks, for gremps of
mobstancess
e.g. acids, IVVI.4A& arts.
We may appraise thoose, two paths separately.
- - - - - - - - - -
This is of value in discovering other compounds
present in
maoks the ortractures of which are hithorto
unknown. It W be applied
to each of the fundamental snake fractions -
acidic, basic or neutral.
The acidic fraction may be expected to consist of
organic acidas mostly
of the paraffinic series, e.g. awtic, propionics
butyric acids otc., and
of phenolic empow3da, derived from the pyrolysis
of the polyphenols and
ta=izs prosent in tbe loaf. The b"ic fraction vill
consist largely
of alkaloids and their dogradation products, i&ile
the neutral fracUon
will be forned from hydrocarbons, -=-a, sorters,
aldehydes, ketones,
sterols (if they survive pyrolysis), torpenes sts.
The character of the saoks from a cigarette vill
be fc-r-med by
the Interaction of those Mrs~ fractions vith the
&mummy orgaus of
Usto and small and idth the pbpiologiml response
of the body. Thus'
the 11-rah-ess of a smoke may be related to the
presence of certain &aids
or of alddWd", especially formaldshydo and
srotonaldsb7dej Us aromatic
Nature my be related to certain tezpamoU
substances, sorters, ketones
and mame 4rdrosarbo"I the physiological. response
of the body will
largely be determined by alkaloids, **J9.
nicotine, &gL-nioctins and their
degradation products and by saide andror snmbnia.
This qualitative ---Iys4s my be astried out in two
main ways.
i) IV-T-tq-" Or"VRA12AMAX On -Iuxim& Or other
absorbents.
This uIll be monsuhat similar to that already
adopt" for the assaW of
bmugTrens# dKeept that other solvent systooss, idU
be employed md fractions,
other than that sontainim bp will be examined.
bufficiont material
Idu Re" to be isolated in a pure torn tor
ni-c-11mis and for
spectrometric aclaination. The VV aboorption
spectrum (daterglaod is
tht VVIAVW win tell a good deal aboat oonjugated
systems, e.g. arommUs
by ft-l-w . Wra, red absorption idll tell us more
about nom-ocnjv4;LW
substane", 0, g' sterols, ofters, tarponse OU.
U) I--- Fl- -a QW-to.2-m-kv *.A N,~Sr
Ph-e-tca-r--kv - VC bjj
the advantag" that it requires vary small asounts
of substances, it wq be
used on volatile or slightly volatile substances,
e.g. b.ps up to )0000,
0-
BATCo document for Province of British Columbia 2
November 1999


and that it in rou_-Jay quantitative in response.
A disadvant4ge is
that if sufficient, aatorial in required for
quantitative sticroanalysis
or spectral cm-ination. of -k- was, then a large
solma oust be used
and some trapping mysten dievised.
Paper chromatograpbr is of value for studying
non-volat4le
rabstano" which any be identified by charagUristia
solour roaations
carried out on the paper. At first night, it would
appear to have
little application toths neutral fraotic% but a47
be of value in
det*oting the premme, of -11-1-40a and degradation
products.
Aw,'unp-t row,'-" for this type of work om&U+,o of
the usual
laboratory glass apparatus for %--A""g the
stibstanoses, chroastographia
oolums *to. A Uvispek should be available for UV
absorption spotra
and w7 eclorizetric work. An infra, red
sp9atromieter would be, of
inostizable value for &W of the classes of
compomdo 34kal to be
ancomntered. VPQ apparatuses are available
oomerciallyp but the
general oonosns~"
, of opb4c, at the resectsympoaim was that it was
still best to 44* one's am apparatus. Far tbis, an
xq)lM~r and
recording potAnticaster are nooessar7. The
detecting oalls om be
constructed easily if they are of the litharouster
tnw.
b) qn-titatiVg A,,.Iv-m4M
This follows the lima of mob that has been. done
before by
other workers. The smaks asy be classified into
gross oompoutntip,
**go acids, bases and andlysom carried out for
Total T"Ile Aoidso
Total Volatile Bases, Total Nitrogen, eto. The
value of this Is
doubtful uAlese it is related to a syvtonatic
progranne of leaf analysis
carried out simultaneously.
It should be possible to apply VPC analysis to the
acidic and
basic fractiom in a quantitative mannar and so
combine the quantitative
and qualitative aspects on theme fractions. The
neutral fraction of
smoke is likely to contain so types of sompounds
as to ask* TPC
of little u" for son* time as a quantitative tool
for this work.
It would appaw that at present wgt advanes ean be
saft met
quickly an ths Qualitative Analysis of moke. M
analysis in a nev
Ual whiek is adaptable to either qualitative or
qumtitatiye anLIpis
of mm*kej, bat it %M take time to perfect the
apparatus required, and
the toohniqme to be aupyrW.
It in wMeated that initially Wrk should be on the
qualit&t-4ve
analysis of sacke using conventional
o1nomategrapbr and that slzultanscusI7
a start be a&& with ecustructing a VPC apparatus
vhiah can be later
Owlayed eitker for qualitative or qaactitatiTo
Procedures.
Wentuallyl, It in envisaged that all analytical
procedures "n
be tied in with a programm of leaf analysis by
grades mWor count;ft"
but this It definitely & long-ters viev.
DGF/VC
CZ>
BATCO document for Province of BritiSh Columbia 2
November 1999