M-f-,
17 __.
of tho pT at i on f I
MoctinS,. nxi April 20 1972 of t, stil,.ivct
to Avoid the cillost.1tic. of
Effects of tol.)Iccb smok,2.
The background of. the work concerning the
tGxicity of tcbacco
smoke has bf-en partly to define toxic
-~ubstati4e:~, in the smoke and
partly to study some biological functicris
influenccd by c):poroire
to tobacco smo*,;.e-. These biological functions
',,Zvc bc?n:
1. Ciliary, naoLverant ir. tho trachea h6tit in
vivo Cnd ia vitro. It 1Y.-I.-t
been shovit that the muount of tobacco cach puf-:
and the different chemical1v dcfin2d substanccs in
t,,.c sm~.>',~c havc
a clear dose response relationship to the effect
on the ciliary iuovQ!-..
IT. TI)e studicr,--of the reaction of free litnj~
cells to tobacco SM01,C
exposure show that after a sl;ort term exposure
the number will dPcr1N":;-_,!.
-1 f, bovi-o-ri 0 Will be continued the nunb I
iv..~,Ase.
cr wil
111. The pulnio.a. --clear.Ince h. C
a ry a s also been stuJied, partly as_ a b a't,:!
r i n-'
clearancc,, partly a5 I parLicul.at.e c1corance.
Both functiors %.%_-re
~'Ocrillstd after exposure -to toba6co =Oke.
A re --a0r:'1,.,b1e
after 'to rvh~lcco Au wi--11 III;
k4 Bike epi ibel i wa -and' wrif)~-r of mit tos
is.
302058919
BATCO document for Province of British Columbia 19
April 1999


Fff,rr of rct!--o wit,, addif i,,,-
Thesc abovc wc:)
~Eioticd 1-,,ur biological NncLioils have bven
ur-d ~L
,
LCSL Lho effect Of so.-.)c addiLivus to tljt!
tobacCb, partly oxf-la:-litic
cirrarr , nrid pai -1), phenN 1-iirJiyl-oxatliazol
MM) . Those subs ances
haVe I-P" f-f-4t!tCd rr~~ C-Ily tit additive to
tobacco but also given
intraperitor-:cally and jn~--rornlly.
1. Dotli oxolimine citratc and MO shuw in
anticiliostatip rffcct
when admini
stered in 311 tIv-- above vicut ioned ways so that
the an
inals
.
.
were ablc 4a smoke a statistically si-gnificni,t
more numbers vf ci.-arettas
after tzearment with vxolamine citrate or PNO
titan without.
M-11- Doth conetrninj, macrophages and leukocytes
il- was possible to-
show a lejF reactior vith smo!~.e frond tobacco
containing about 1.52 FMO
than fror4,to"al S:ankc after a fou*r weeks
exposure to four c.-Carettes a
day.
Ad 111. Concerning the effect-on pulmonary
clearancd if was Shown tjat
l
both radioactive And viable coli bacrei
ia. disappeared a littl.e,,quicker
after exprisure to' tobacco srpohc vitIl 1"No than
without MO.
Ad IV. AfLer six weeks exposure to tobacco smoke
with No a 5 addttive
j
the following results could be Shw'n-
a-) The incrqAu;-aL tl:~-, nu~alwr -C
:-,-b1-ct-nz~'.Ti wAi, tt,'u I t i e E sthan aitur
00
exposure to tobacco SMGke-:WW,0Ut PHO, h) so
was-the thickness of the
epithc) it1::1. C) 1he numbcr t?f,1:1itt',ai!i
Lhtl
Sr"p was,~cxposed Lo tobacco with YN,0 as r-OM 1)
ar a Wth-
-ve
302058920
BATCo document for Province of British Columbia 19
April 1999


3.
In further evaluating the protectivc- cf1ects of
MMO guinei pifis
have been exposed t;' heavy co;acvntratL~t, of
acrolein for about 5 ninutes.
Thereafter Lhe AAimml-bm,,e bne-n -:rcnt-re
frcqu-,citly,
perorally-tip to 80 hou-,;& after L~,c end of Lhc
exposuru. After that zi----
the ani~iials were sacrified and t"-chca, ~rvnchi
anl lUll,,S W,:~rL hi.S~C-
Pathologically examined. There w3s in mort c-asL'S
a statistically &:L-nificai:
A
dif,forence in the degree of infl.-i-mration in
Cie tissue indicating a
stron,3 protecti-ve errL-et Of F-30.
302058921
BATCo document for Province of British Columbia 19
April 1999