""Wst WNT1,14T OF CIGMmms (tAfg.120 ctti&aKa trs) R.,I.c,t fw "J"i.t. ."Id be 4jjC11,j W G. 1'. L., (lo"'I IJO II.Rti.Ud-, A-C, M- T.W .J- meh-m. 02130, USA Mh MI,- nu- 1. RMHUNCrS A 24-0 1-2 29 %-11, J. A. tbid, p. 4 2 7 24-5 4-; 17-7 1 3. J. S,MJ14 NUC.11, A. 1. Y. N,h. D-. 196% M, 287. I U'S 4-0 4-4 4U..., M., Q.-I. V. AU1 Lv-. 1962.02,737. 2 28-0 4-2 42 15.8 11: Pfi-i: F., 6--. F. F. .4,,h, -1. 111A. 105,0. 1, 6SI. 3 20.0 6. A.d-... A. F., J,.. J. A.. Jkkm, P.. F,,,Pk,,, A. G. , 1 2 27-0 19-0 .0 2-9 .,,; 1'":1, 1'!-; !"n, T. .1. 1).W., it., I.M. A. .- P, -d. 1. V.164. 11". It. ... "it). A. It. ail- 1, 1460, 9. Mithell, K. S., Vin-a. T. N.. Alter, G. F. .7, Am. md. Ast. 0 23 4.2 27 1961. 1118. 12. 231 4 :2 15-4 'C', II-W... J. A.. Anckr... A. J,., W. L.. A. G. A- X- -p. A,. 19W. 03, 78 2.6 2-4 H. A.. 21.0 3-1 15.0 1961; 14, 236. 3 3.0 12. S.1utin.t. V. IL dwfyl. Chtm. 1933, X% 493. P 1 23-0. _ ] ASSOCIATION BETWEEN ELEVATED 16-0 M4. 217 (10016) 3-6 (M%) 3.3(15%) ~8 9!0 HEPATIC AVATER-SOLUBLE PROTEIN. ... 1 b The vatzI sinount af c3dnium cont.in&J in the ciomtoa sinnke WH S AND CURONIC 130UND CADMIUM LE *L dc,[.d kom -,h *..d Wk IM "11.11MM by ..bU-aCd.9 1b. pron tg- for ash plus Idgin (rWU ttw Uttaft ~t%,ML~r the BRONCHMS ANWOR UMPHYSEMA Gbows P. IA%Yis supmatards were gargtiy boiled down to 15 mi., blevched Ddparip"ent of Afaritria Afedica and uldvemry with 5 W. of 50 volume % hydrogen peroxide, and sign- 1. ow aisd Siobitill GencrX Hospital GlaWow MI of Glar merzd for 10 rulnuta, Afmr cooling, the acid solution , g wa partially bauts:411"d with -3 N sodium. hydroxide Huatt Lyu. . usinq 41tvauglAgliae as &a indieftw and tiuAdaq to thm Afm &0j-[yqwW*j~%v, Mds.PO~dikrt UfWtfd, U-0d the colora changed from pink to yellow, Tho final volume MILLER STEPMN tru then adjustO to 25 ml. with distilled vrster. 'Mis proem was repeated for the fUtm and the a*- Dep.,pnomt of Mweria AWca wd 77iagnapautk4, firom 18 imokcd c1gamims. Uiia"ty ofolaew -Me cadmium copper and 4m - levels * CAdinium *as estimated by Salmortn's mlorimetrk Sumprign-Y in cw=trat u2 ter-soluble protein inenhiWo" A memm of rqsrodut~ was obtained -by Y ruxulan 4%,cr Spectaw" exEr"" PT r mu WX' pulora"S cadwatiprts en, whote cizarcttes, asb. =d.. were estin-Ated Eumcts front-patiem dying with filten obtained ftm vgorc., then one -pacii: of mch brand . of vinfixVU.'i vi*nt~m 4 chrorde bimichitis midAgr ettaphysema wre fouhd to h"e.ainews cadminm WvA greatto then dwege -7 U table -sbows tM imt-iom con Ica of -Whow It -0-0=~ . imes that 1bund in the Icest (Ps, u1 WMok4 ciprMa, Ast4 mVJ ffitm after Smoking Of ilk at- WWe cimWite -T.Az-.tW1ogh=J Tak-of 5:gdmitm~- 1~ bmnact- dsvx - 'ttow one pack is compared with others of the ame remams uncerWa. At With cadirgiain h undetectable Wafi,4 Takh*-thiii 111110,0aMidCtatiOU, the culmium jlk hutml~ t*1K.V1 joi~-44 ' , q meml of diffb"= bmid packs. does-w vary ly ammtdwes beiiiuii Inta U_ c, x6aU excredon gtadvW _~ anliniin i 6T mnoked d~kMres is rak&ply coamnt =4 refwf , . -'s"S 15-7201,'4r of 6c v4smolwd.dprettep L=Cnt_ 4-1 rtiv_ of t6h, is stotid in the Jim.` The xautcm of. a6 diiifatkiilg ai~r&wtiirmfil -ra~st. of the vaghniwn fn:dPr;0r_ tobacw.pvsses .0M- ' water contairging cii4mittro7n;tain the iwag,ltl she nv*r lie - 'b Cr t'Diripau lo vd1h the o U- 9A-,, in h 4~rg~. Thii Is and khArge ir~ emoums dl I A to the remirpropon an --thm ow the tetrgperawm at the igniw out or a dietary laraW cso*d the botiftVoint of va unt" dmi The fOxic'-df-Um Of w," in 01671Q.0 Timirdmimion-imd -the i4baffption havc b W du j0 . 14110 VA 10--20 cip : "of the avivirittm, coatrjued in .dci"unqnt 6f amc'.brbnch itis; p1muguorgit4, _ &T#Qft~ bY ad'MvidiM strifthi& more fli, ift 20 eqiXA2Uv M z = w" 7 iibotantW iwtixii in the 2~6uviuhztton of tWS'L- 111'riarlut "keO. ~_ftnupbr abmirl-by. ft chrodic'-, Ck4ne4t- In ow "body. .1maUtdoft of chron! m~aut tu IM ft&ai: the- N4tkw#l. I few luitkutt v;W. 302058346 BATCo document for Province of BritiSh Columbia 19 April 1999 -2 - V -i " 7 I 1331 We have investiratcd the relation between po~,t_ CADMIUM COPPER MON nim irm diann,ises, and ilkc codiniwn, c4Dplwr, and iron cnnic:w -jfwntcr-solut~c protchi t:xuacts prepared frani a ram, ma wries of.d.,Ii -adaver livcrs. 1800- Wt,-.I. and Methods 1600- Appiovimntrly 50 p- of liver was obtained at nccrepsy ?D- 1400- Ohm 4-6 h ... o fdc,.,tl, from sixty adult p.ticntx whobad died J-ri . -ti"y t I1nth.)Cg;,L% %,cxc aA4d to 01,4- or-ik. --r!,-. ff 1!..:z .;_- L... 10un.e ad.11t vwr- n1 orl- "anlinationa. "r.-SpcCriva of the cause of death. 1000. of"0, %azi,ple ww usually stnricd n the day of 6c nceropsy. If this was not pmsible. the whole 4 boo liver mmplc was storcd in a &cp freeze (-20'C) utail a time con~cnicnt tot prccuAing. k : I-reparvoi- of sapk. c A 4 p~. cntratc.J cxir-.4t of the water-soluble protein 20, 406 finct o=, frce of cdlular debris) was prqmrcd fium citch Ii- ming lk~xnogcinstion and di.lyis teehniq-* wm tfamd at -ZVC pd se t to 200- 10 n l ba L ,err-I In tchcs of tm for estimation of trace-metal conicni. a- bochaftwal Method, A s Total protein content was eadirAted by a biuret pro- 13totribWlen of cadgah~ and L-n (jag. P~ W V~cto) to Uvvr -dure 0 and h=noglubin was determitned by Lewis' rx-as from simy paticam dying with (13) and withwt (A) method.'' Cadmium, voWa, and Iran were measured by mantion or h .. 1. broachiti. -41.r -pky-.. atomic-absorption, speeftophowmetry wing a Perkin- Mince 303 instrument v6kh . sidglo-slat burner and if, elements assayed, expressed as parts per rmllkm (p.p.m.) for Cal; in 1% absceptionw43 0-037 Or ftiltilidiii, 613 f1ar copp", and scrtyle" ftamc~ S Itivity of the apparatus fer the AracA 9-16 fox iron.- TW et:vam WiT douted 1110 W" TAJ3).L~~- Z==hrMk uAr it t *"T-?A6am tvivw..a jwmwm saline solution before they were put In Lhespcu0ph-t- 7 iy4m TM 60 SANgkim mciwp= meter. AR results vvere exprasscl as pg. per g. noo-luemeiglobin-- N. pmteln. All estimations wcre &ac Without prior Itmwitifte iftt~d Ch-&- b~. ~. WW..jA#_ 12- post. wortem diagnosis. FindbWS u.,,hy- . . . . V I N-1---y ro_._Q1_ _ 1. .. I Nectopzies were done hy staff of the poduAagy depart- VW-y 1,bc-1o1t1 _ .- I -mmt, Siobbill Cm;ezal Hospitni. Glasgow. The posr- (a pai-dr 10 rnonern diagisoso rar each patient was obMinad by rafer- el", to the firAl n6rainy rcpart. The cause of dkwh in some Instances was astritnued to a singk, patholo4i-l Cntiq. at b in jmmcases twa or rnori: distinct 1,904agical -A 'Ilie final liver extrisas ~(Mtajncmd on, evelev fiLl g. L of, protein per-IM vil. frxxiic &k4i.T-33) of- whkh O-W g. -.7'f SAA, twfnoglobin SON" that o-20't. hoeirw timinto of cadonfuto An*d glabla coataiDs undetWaW%W X.-F. fWa. (-te PY__ THe Nruarnin.tion'Otihe liver C=3CUk '11th I'Xyik* I .2 Wra amun ts of jiserAD&Wtu VierermV, was not .2 favor coxiii-Ibuting to the kvch of the traco Scritem JL Cal" fousid in the ej"r", fthwtj .baatarn TalAc I shows t1w jiversity of the diaposh jecarded , V-1kii-M. Uf,;,#_` far,tbed.*~Wkiits. Thc III diag"es-"CUoupod .4 u -five broad cqtc&qrica,_Thg dbaauaew~widtfit "Ch em? had inore-thanone4l"Irs Wid thl a iongle eittcioty (Cg, the cornt*qtim of ..0"IMStry-mm thromtUs and arhiro"fosis, both wn. catc, - fir, qory v)~ Ohem had a 0offibination.of. of -.UMt-WA in. pwasiw ft",-hm IMA .0' diawwes ffm move than a &&Vry (~*.A firond-. tht 1.11 a its. "W., V.bqa a- 0 C cat -a, -Acchknt., W. prwumonia. eixnj4i,=qinV a ctron 302058347 BATCo document for Province of British Columbia 19 April 1999 77 T A 0-ftit, -N0 IR-?' LL"I'L- (lg. M a. mto'.914; IN WATER-501.11111.3 LIVER-PROTILIN IN'tRACTS FROM PA'I'IbN" WIT11 1.11t.1.1 A -f-MIVTI~M IIIAti-IN 01' CJIRUNIC --a"T's E."tys'.A h-a. t-ha~- 'h Cd-.- 38 F76 9100 7-2 10 01-W hmuchiti, wUh emphyi-i 1.2 39-8 C 7 'W~ 11.5 1-4 7 W-4 'b , j.. 310 14.i 1'632, 114301 Pd. ~y t 38 110,6 1.7 37 105 15-9 36 '231 01 h. (H- V) 40 W-5 1-7 $0 101 15-1 38 226 St 20 141) -0-9 rib 223 0 A 60112 152 TAALI ITI--munim, COPPFit, AND IROW JJVUS (jAg. jw-~ g, PROT.,W) IN WAIMR-SOLU11LE LIV-PROTEIN EXIRACrS 1A.-M, PAVEIM WITH AND Wlnl= A POST-MORTEM DIAGNOSIS OF CtRONIC URO?irJIITIS ANI)jOk UIPTIV"MA IILLA7LD TO OTIKR NI=ROPSY 1"INDINGS Pat.. kb 0-1, b-h4b W'.' ~hy- -ph- J C.'WA_ .1 C-Pp. Nu. hiftni-M. N..'M..1'b,. N.Im-I.S.- 1141 132-9 16-4 7123 "141 36 1,4 12-1 2:7 1 W 11 1:7 12M 1 6 101 1 144,2 11 .1 "7 1" 5 97 24 t 170 53 Aw. kdcctiw (U N-0-04 OV) 420-2 8- 11 I'M4418 249 14 :1. 2-314 31 3 141 33 1 " ;'.j91136 U4 .. 2 340 2 - 91 9 3 W 28 9 351 164 2 c'aUt'a'gotIcs uand M). TUM pauena caftseque4tly The high levek of liver 4miliniubi cbtdd be the loM- *0 Wcoun%M Inoft thft mx,& t" k-bilt Of e6t..V9, ve ifiSke. ofte Twdsible zoni~e of increased intake aMcting lorge sectiorn aftbe po;"l Trace-incul levels found In the tiva extracts from Ia- padeems with nccmpiy mention of chronic bronchitis don is aft pollutiom Air-pollution varies considerably amVur cnIftiem -A-A compared with those values for fmm one eirea to another,"' being most pronounced in patients without axntkm of these diseucs. heAvily industrialiied me=-- The 4atients. in our- serics tived on Clydcside, atiom Or. theth In Glasrdw or level in the chrouic-bronchi tis and/or emphysema group Its suburbs: amne auy have woirkcd with cadnibm or Ia OVCr thmc~ tlauv tLU ruod in th, rcouiirio&i. No haw lived 4k)wi3wkJ frorn factoric froriv which such diftral9b on*rgcd for copper or inat. Wilcoxon 'cadodum fumes might escaln into tbr atnioWhem -tw&-sample, rank-sim, teats JI were carried out an the occupatidnkI -histories wem. not aviii"Ie, the -s tor cach-elavirm-studied coInpining the Tomierits - - valux Inds"ce of stidi famrs on the vride ranre of with chtonlo~ *nchitit,aud~= cm!?hyscMA with thc, jai solvbk,protchi-bound vadovin.livels found ~flie: LAL For cadmiusn., the approxiniat. nerawl &-ime -live, extriacts cannot be flulty evaluated. Another wn.4-ft (An elm r, vahte - intake, Or -potemmW aode= of cuftniurn ecting a stedW bivaks down Ut sucIt emenw valacs, but is cei 61nTy Mr' inv I s -wh,ble es Ication wa conn"d to rmlcr - . "!.) !trast, tl~ kRi 04 04M 1 fA it twq~t~" -In oq~ - in. U..n 1i`vcr-:A siailnr_ I ..64 with - cownutty Q~MTe&iq'~ br the norma cmupuhon for toig hepatic cadmitm 1evcIs wouto be 0-29 rt9wtc&Jn the total cadmatimi content. 'Hutuan Iuna~ Table tit showi a comparbon ofthe levels of the three-, - m-sumbli: CWAbU :tRWI-bur f U= 4"W$ WWALLthe swific disc= ftwiodck 14M -'eadinium.341 Rhw* vadmforn-6 it b miia mq~ .f vuo , , s in at ents dying Of chronic- bronchitis havc. jiw= vdtbzid %vitlioutchronic brouchitis andW emphysenil kelmric colmium levcAs than tbose j"ents without - to both octupul"t ivid snukii - --with refct~ 119 sutIrmcritioh. -his~~t Yield Valwble WWWWWd lafurmation.' The liva- Is otte ot ft lWy *qMj w4kh. st", Wtriluw~ coppaf~ and imn, ht qWedable Rniotmm'. V-P-4 4M. HoViml. Mr. J. A. G. Vi&r. Dhta P-hitj L.I., rj We bwc4*vuC*x4he auaaA zadn"m x4wp;,X~of - W~ i ~d ~ id h ~ t-xAor cm o tj v Iffistic ftons V*ms w v po I rutatio. of IcW. andlw cniL*y-;, it- Rjq. t. *(mM ba WJnwd W Q~ V. J~.:. substarAWY higlier-thin the mewl lcva-fqufkd in. cxtracti, fmm othcr t1kAM_ koww.~ 302058348 BATCo document for Province of British Columbia 19 April 1999 11-111ECT OF BLOODAIANK STOUAGE ON LEXCOCIA"M FUNGrION ]HIFFREY MCCULLOUGII* SAIYORA ). HFVr~'?? EDMOND J. YUNIS PAUL G. QUIL J)cp,,rw,.,Nt. of Lb,-,aooKr Medc,m and Universily of Afi".'wra f I'lie Imcterividal Tnivity of pol,,-mor- (P.M.N.S) arld the ability of lymphocytes to rcpond to phyv~+.,e- maeglutinin (p.n.A.) were sitidied in blood drawn under stanslard bl,iod-hank conditions into acid- citratc-clextrose anticoagulant tuid Feared at 4'C. 'I here wit-t no-loss of bactcridlLul aLLiv4y during the first 24 hours of storage, and bactcridU activity was not completely lost unffl aiter 4 days, of stnrage. 1-,ym- phorytes lost the ability Lo rovowi CA Ft days of storage although inorphologicilly rjouw lymphocytcs were present in blocdstorcd for 21 clays. Irradiation with up to 6000 rwirgens reduccd. but .+d not climinatc. the lyalphocyw response to P.Ff.A. and did not affect the bactericidal cnpacity of the i%mx.s. Thest results sogren that ffinctionally wive P.m.N.s are present ia bank-blood during the first few days of storage or may be separated and stored for periods considembly kaga than prcviou%ly indicated. The presence of morphologically normal IyfnPhocytel and fv!~ponslvcMs w P.".. stimulatiou indicate that any bank-blood %ithin. the 21--day shelf-life inay be the source of a potential graft- versus-host reaction when tm-rfujed to paticilEs with impnired immung: responses. Irradiation of the been conrAdercd 11-tr fianc-ional polynimphorgurlear Jruucyles burvive in bank-blood fur only a kw hours " mid Jyn,pl-(.Vl'% lor lmtg~r.' flu.- pital inputiunt,, nigh ticitocricies Of ifnIfILM010riC.31 computelice ible to congmitAl vause" fleopla41, untincoplastic ificrapy, or traraunusupprcasion experi- efw-_ severe fuLterial COMPIlCaLiUns and Are sus- ccp6ble to graft- versus-hc~i rcActious; litirre precise knowladge of the m-evivol -- f"rrtinnnt '"",L- i.- of great Jnip6naucc. We: luve invc:~tigwcd the WcicricidRI cupacity of pulymorpho Ll,.r letic,- 17 cyws during storage and dcterniinM the functional capacity and ritirviral of lymphocytes in Unk-bluod during stontge and following irrndintion. Methods Sourm and Sroro;;z rf Blood w.1 Sparwimi of Ltwerytes "n dr-wz. Lud,:, bloW-bank cnndi- lions 4 into plastic bags (Fenw-A JA-51i) containag 67-5 mi.. acid-citrate- dr;u rase (N.J.H. formula A) (A.cj,.-A.) antimagulant. 'The units of blood wcw clatkied into 31 D 60 wl. uliquots usuig a I-enwal phismi rxfr2mr and stored in 150 rul. p1mic bags (F.-nwaJ TA-3) in a blood- bank refrigerator at 4'C:. When each aliquot -s go b~ tcucJ, the blond was Withdrawn through the exit portal, mixed with 3 mi. of &,'~ clinical dtxtran (Cutw Labon- tories, Berkeley, California) and incubated fr 45 to 60 minutes at 37~C. Thc erytivocyi" sedimented and the plusnia containing leucocyres, plateWs. eikl a ** ertthro- cylvi, was withduwa iagd. a skWw tcreiv4op covIturc- tube, The lawocyte-rich - plasou was ccritrifluged at 10 W rp.m. for 10 minutes (Sorrall modd GLC-1). The plaiana wat discir(Ic-if tind the huwqtcs WCr-L.WA&hLd_.. with 5 -ml, of 0-85'.'~ saline solution. blood appatently -d-Ovs not pro= aw;- tMa bizard, The method a Al"ec 6 as ANodified by Quit tt jil.' - was uwd for evehuming P.A0. tnetrrk4ftl car-u:q"_ 71'Q 'nin stirvival and functional caDacky of erythrog:3U3 mediml comrstcO essentially in incubating 5 x ](0 P.&M4.3 , platelets, and plosma fwADn in bank-blood have bccn with 2it equal number of Staph3iacmwtv mtretu strAin 5W_ A ' extensivcly studied. but rt; y littk it known about it, the IuLphase of growth. ftmh h4zm human S"m ' ' - . the suivi-val of funidonal leucocl,-ics. stored un&r waq nsed in all eW_6rn,:nt% as a izaadard w rw u ot opuinim a Vle phasoc tic fflLxtu _standards blood-bank Conditions. It -has gorc::~Zy m Were y mm atcd with erkwver- end rotation, at 10 r.pm- in sterile plimic tubes (12 % 75 .. redcou plastic Aspwabl;).' to W."=:m3ax4nu*~ 'C~tacl - - -- bawcera bacter" -- 41cirrmined immediately zifter'bactcrIa weft addeld to the* phaLoL5 iic mixture &rd dwing iac'ml*tion.' by r=ovinR - J , TIMA. -L IL -MtW Skdim In hiefficki- zto. I MGJ~ -4hQuots fiun P.bt; M 'Wilts a cz-byktcd . pUnnum loop.. Conuols mrWswd of tubes with WneriA t-k _3b1wi,.ACV.A1 , Taz~ www ' .7 3, It"wrt of I.C-A'p. Cjw4mktct It -n rMakukk Da 14"nttgaa wul opivw4i withem bacteria, and vubcs itk ubowd with- . out autation to dctcttn:ne erxtnceLt!2tarbactcAcidar-.i,-ti%-i;*. 4. kL A., Haksw~ J. 1, t-v~v~ J, Q'Iijimt" :W mi"u"s, -W Agdotis.- Auld .:1 40 t'"&"X' cit dUA. A,e& N,4 U&h 5 V. A 10. 224k, 23,2" L'.Pl."' P. H., fflxrr.~_ H. CW. Mid. Iftt$, tfk% Tl~_ reswr" of, ljimphw) tes so stimulation by phy o- t Y. Mn#PL Afed. 19117 %it A"lV4udnW oat . 9. W14vv, L. AM~A: A,~k i~d. H4A, tM 11 27 Torpncy and Mueller.' Fo119Wi;*.Sq0t" . , PlaWILU, lcaoo4zil Were wmhv4 -anct Wfth ftuiW~ Ah 12. AULgag w -W. J Lw*rb r . . 11 . Lxwnm n0 44.P, 1. 4N.. Rd. 2M. ' 1`164%fwl