TD7 Group Research & Development Centre, c I,-, ZIE D British-American Tobacco Co. Ltd., SOUTHAMPTON. sRm/cAL/46E 29th November 1978. EXAMINATION OF AQUEOUS EXTRACTS OF TOBACCO ASS A SOURCE OF TOBACCO FLAVOUR MATERIALS (Report No: RD.1636 Restricted) SUMMARY In order to develop processes to enhance and control the tobacco flavour of low tar cigarettes, it is necessary to acquire a much clearer understanding regarding which of the natural constituents of tobacco are important for the tobacco character of smoke. This report describes work to investigate whether it is possible to generate a crude concentrate of tobacco flavour constituents by aqueous extraction of tobacco. Aqueous extracts of high flavour grades of flue-cured, Burley and Oriental tobaccos were obtained and evaluated for their flavour potential on low tar flue- cured and U.S. blended products, by a subjective assessment procedure which involved the use of a questionnaire. In order to gain some measure of the flavour differences that the smokers were able to perceive, this evaluation procedure was initially used to obtain a series of relative flavour assessments of Merit and VICEROY EXTRA MILD. The results of these assessments showed that the smokers did indeed find a relatively higher level of tobacco flavour in Merit (confidence level >95Z) which appeared to -be mainly associated with the Merit tobacco cz:) blend. However.. in general the 9mokers were unable to find any increase C.0 cr% L...4 1 ~0 BATCo document for Province of BritiSh Columbia 23 April 1999 -2- in the tobacco flavour of either a flue-cured or a U.S. blended cigarette, as a result of treating the cigarettes with up to 5% of each of the extracts. This loading-is approximately equivalent to the addition of flavour components from an extra 250 mg of tobacco (or a 33% increase in the level of potential flavour components), assuming that all the flavour components had been removed from the tobacco by aqueous extraction. It was concluded that aqueous extraction of tobacco does not offer a useful preliminary method for isolating constituents that are important for tobacco flavour. It therefore would seem unlikely that the isolation of a tobacco flavour fraction from waste tobacco, for use on low tar cigarettes, can be achieved by an aqueous extraction procedure. C__ CIL C71 BATCo document for Province of BritiSh Columbia 23 April 1999