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DETERP41NATION OF MENTHOL
IN MAINSTREAM SMOKE
REPORT NO, T. 36 JUNE 1971
AUTHORS: T. 1. WILSON
C. G. BUTLER
R. J. GRIFFITHS
ISSUED BY: R. G. Nicholls JOB NO.: 9155
DISTRIBUTION:
Mr. G. A. Preston/Mr. P. M. Denton copy No. 1
Mr. N. P. Emery 2
Sir Charles Ellis, F. R. S. 3
Dr. S. J. Green (3) 4,5,6
Dr D. G. Felton 7
Dr. 1. W. Hughes a
Dr. R. A. Sanford (2) 9,10
Mr. R. S. wade (2) 11,12
Herrn H. E. Sottorf (2) 13,14
Mr. J. E. Sismey 15
Mr. S. K. Keshava 16
Research & Development Department Library (2) 17,18
COPY NO.:
BATCO document for Province of British Columbia 19 April 1999

SUMMARY
A method for the quantitative determination of menthol
in cigarette smoke and on filters has been developed. In this
method twenty cigarettes are smoked onto a Cambridge filter
which is then extracted with a hexane or carbon disulphide
solution containing a known amount of internal standard. Gas
chromatographic analysis is used to measure the concentration
of menthol in the solution. Using this method a coefficient
of variation of 4.5% was obtained.
The utility, reproducibility and limitations of the
method have been examined by application to both laboratory
and production samples.
lop-,
The method was applied to determining the amount of
menthol delivered per puff. menthol level was found to
increase with puff number. During the first three puffs the
level increased rapidly and thereafter continued to increase
but at a reduced rate. Many smokers claim they experience a
decrease in menthol level during smoking, but it seems
probable from these results that this effect is physiological.
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BATCO document for Province of British Columbia 19 April 1999