Research Laboratory Report No. 151
Co0y No. 13
0 CENTRE
IMPERIAL TOBACCO LIMITED
RESEARCH LABORATORY
MONTREAL
USE OF THE NITROMETI HAN E FRACTION INDEX (NMFI) AS AN
INDICATOR OF-BIOLOGICAL ACTIVITY Of 5 KE
RESTRICTED
Authors: M.P. Scherbak and T.A. Smith
Project No.: T-7092
Issued By: Dr. T. A. Smith Date issued: April 12, 1977.
DISTRIBUTION:
Copy"No. 1. Library 15. Dr. R. A. Sanford
2. Mr. R.N. Gibb 16. Dr. R. A. Sanford
.3. Mr. R. S. Wade 17. Mr. R. G. Nicholls
4. Mr. S.M. Candlish 18. Mr. R. G. Nicholls
S. Dr. T. A. Smith 19. Dr. M. W. Bi I imori a
6. W. M.P. Scherbak 20. Dr. P.J. Dunn
7. Dr. S.J. Green 21. Mr. A. F. EL Naggar
S. Mr. R.N. Sottorf 22. Mr. E. R. Freieslehen
9.' Dr. F. Seehofer 23. Ms. J. Johnson
10. Dr. W.B. Fordyce 24. Mr. R. L. Rice
11. Dr. D. G. Felton 25. Mr. A. Schaffer
12. Dr. D. G. Felton 26. Mr. E. P. Gage
13" Dr. C. 1. Ayres v' 27. Mr. W.A. Gray
14. Dr. I.W. Hughes 28. 'Mr. A. I. Kali
29. Mr. W. J. Ross
30. Mr. N.D. Maclennan
31. Mr. C. J. Brown cm
BATCo document for Province of British Columbia 19 April 1999

USE OF THE NITROMETRANE FRACTION INDEX (NWI) AS AN
INDICATOR OF BIOLOGICAL ACTIVITY OF SMOKE
SUMMARY
The Nitromethane Fraction Index (NNFI) has been established In
this laboratory using the method developed by BAT (Hamburg) and wdi-
fled by GR A DC (Southampton). It is a relatively rapid and inexpensive
test which can give a useful indication of relative biological activity,
as assessed by mouse skin painting test. The test is to be kept confi-
dential within the BAT group.
It is recommended that the NWI test be applied to samples in
future projects where there is a possibility of a change In the biolo-
gical activity of the smoke.
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BATCo document for Province of British Columbia 19 April 1999