Dr. R.R. Baker W.D.E. Irwin
WDEVBTM/ 12 June 1989
Project DAY - Other None Interaction
With reference to your note of 7 June, I shall make the following preliminary comments
with regard to your numbered items, in reverse order.
6. David Matkin is writing up the proteinase work which could include a description
of the scaled-up technique.
5. This raises a number of points which David Matkin may be in a better position
to answer.
(i) Presumably mixed enzymes could remove more protein if the two
enzymes attacked different kinds of protein structure.
(ii) Do we want to remove all the protein?
(iii) Is everything which analyses as protein nitrogen actually protein
nitrogen?
(iv) Protein may be bound with other molecular systems rendering it
protected.
4. Can I assume that methanol pretreatment is used to remove flavour etc. for
subsequent add-back after the insoluble portion is treated with enzymes and
resulting amino-acids extracted? If so, fine, but this is more at the application
end of the work. which we are not considering at present. I suppose that once
the protein is converted toamino acids it may be possible to use some separation
technique similar in principle to that used by the Germans for polyphenols, i.e.
somethingwhichwouldsolectivelyromoveaminoacids. However. the Germans
probably removed a lot more than the claimed polyphenols.
J-1-6
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3. The extracts have not bow analysed. but presumably contain amino acids plus
other substances taken into solution by side effects of the treatment.
2. Use a carbon filter to reduce but not got rid of some aldehydes, but efficiency
for acetWdehyde is low relative to acrolein, and other higher boilers.
I . In ITL 132, 5 different sheet types were examined including Gerlach. Of the 5
samples, Gerlach had the highest surface area and the least ability to oxidise
ascorbate. It also had the least ability to reduce dic I
don't know where Mat leaves us. Formaldehyde and acetaidehyde results were
not high relative to the other sheets which would be a sign of a more oxidised
smoke. I also note other differences, e.g. very low phenol concentration in the
Gerlach condensate. There are many other possible explanations, both at the
tobacco and smoke chemistry level, without resorting to surface area and other
physical effects.
W. Q. E. Irwin
cc: Dr. D.A. Matkin
Dr. E.D. Massey
Dr. R.L Prowse
4b.
C)
N)
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