AN OUTLINE OF FUTURE STUDIES
(A) In-house studies
(1) Tobacco smoke condensates would be subjected to limited fractionation
procedures and the biological activity of each fraction and of the whole
smoke determined by the Ames test. The condensates would also be separated
by thin-layer chromatography and mutagenicity detected directly on the
plates. The smoke condensates would be prepared as follows:
(i) from different tobacco types.
(U) from different smoking regimes.
(iii) from sidestream smoke.
(iv) different products (design parameters changed).
(v) and possibly, from different modes of smoking (es. cigars,
cigarettes and pipe smoking).
(2) Effect of casings and flavourings on mutagenicity of smoke condensate
would be studied to provide useful information for dialogues with
the Department of Health, Canada.
(3) Mutagenicity of cigarettes made from chemically and microbiologically
modified tobaccos would be studied in order to identify conditions
leading to lowering of mutagenic activity.
(4) Quantitative comparisons between ITL products and corresponding
opposition brands, as well as products from other countries would
also be made.
(B) C. T. M. C. funded studies at McGill University
(1) Work will continue on the investigation of the effect of smoke
exposure on perinatal animals. Tbese studies are aimed at seeing
whether exposure to cigarette smoke results in smoke components
reaching the pups, and if so, whether via the placenta or breast
milk. AHH will be used as the biochemical indicator.
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(2) Work on the nature of the mixed function oxidases induced by
tobacco smoke and involved in the conversion of promutagens to
mutagenic intermediates will be explored further. In this connec-
tion it will also be determined if tobacco smoke exposure changes
levels of amine oxidase in rodent tissues.
(3) A programme of work on exposure of animals to ambiant smoke has been
initiated. This programme will also use AHH as the indicator.
(4) Work to develop a micronucleus test is anticipated. The object
is to obtain a short-term site specific test for tobacco smoke.
September 15, 1982.
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BATCo document for Province of British Columbia 15 April 1999