i MP. rog KAt A
Imperial Tobacco Limited/Limit6e
RESEARCH AND DEVELOPMENT DIVISION
MONTREAL
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THIS REPORT MUST NOT BE COPIED OR SHOWN TO UNAUTHORIZED PERSONS.
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BATCo document for Province of British Columbia 14 April 1999

COPY NO.
IMMM TOBA= LTD.
RZSZARCS S DZV=PKERT DInSION
14mav=
PROG= XCMT
JMM 1988 DZC@- INS
DISTRIMMM:
Dr. P.J. Dun COPY NO. I
Dr. S.R. Massey 2
Kr. A.L. Heard 3
Dr. R. Binng 4, S
Kr. L. Reynolds 6, 7
Kr. G. XcGr"or 8, 9
Dr. R. Rittershaus
Dr. 1. Kauxrh
Dr. C.J.P. de Siqueira 12
LibrarZ 13, 14, 15, 16, 17.
4@w
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NM ARM 01
PROD= FS-IWjk= a DEVELOPKW
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BATCo document for Province of British Columbia 14 April 1999

2
RECMW TYPE : P
SIM TYPE : S
SECURITY CM1
7WING BODY
0RGWZ=ON ITL CANADA
aRm HUNZER
WCAL PROJECT NMMER(S) : T-3204
PROW'ECT TITLE : SATURN
PERSON RZSPWS1= : SMITH, T.A.
U-MIRT : 1988
PROZECT DESCRIPTION : To acquire an understanding and Wir-41tion,
from a consumer perspective, of an a---ay of
product taste, sensory and physical
characteristics that would appeal to and
create preference within various seg=ants of
the smoking population.
SCOPE : GROUP RMEVANT
DZM : AMM/DXV=PMEMT
FUNCTION : MAMTZNG
OBJECTIVE : SU]3J-EC-.IVE
MUSTER : PRODUCT RES/DEV
DATE REVIZY WRITTIME4 : January 1989
REVIEW TITLE : SATURN
REVIEW TE= : Work to date has concent=atod an the
achievement of a rang* of cigarette s=ke
tastes/aroma which were, in most cases,
overtly different fr-cm --hs normal co-rservative
taste of Canadian ciqn-roczes. They were used
as signals for amelioration of smoke
i--ritat!-on/aftertasta, or for their own
benefits. Broad taste categories desired were
berry (fruit), smoky, maple, vanilla (pipe
tobaccos), and non-Virginia blended, to be
achlov*d using flavcm--s and/or tobac=s. The
work relied heavily on suggestions from
colleagues in the BAT Group. Flavourz and
levels were screened internally, and promising
candidates were manufac-t4red into samples for
testing in a series of focus groups of
consumers, supported by concept boards to
explore ways of coommicatinq their
advantages. Insights from theme groups
suggested that some improvements would be
welcome in mouth cleanliness while inhaling,
little or = aftertaste, a reduction in the
level of environmental tobacco smake (even
some modified arm&), and some changes Li
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BATCo document for Province of British Columbia 14 April 1999

3
taste, provided they are subtle. Spearmint,
elderberry, passionfrait and strawbarrr hav*
shown son promise. Bowever it will be
difficult to persuade soWcars to even try
flavoured products, unless the products also
bring significant bansfits to the smokan *-q-
health, social, cost (mall&r trial packs).
LN
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BATCo document for Province of British Columbia 14 April 1999

4
RECORD TYPE : P
SUB TYPE : S
SECURITY CODE
rmnro Dovy
ORCMIZLTION ITL CANADA
GROUP NUMBER
LOCAL PROJECT NMWER(S) : T-5350-3
p.qWEC2 Tr= : HAMSTER
PERSON RESPONST372 LESLOND, P. Ir.
EFIORT 19M
PROJECT DESCRIPT=N To explore the off acts of I=rporatimq steam
treated Stan (WS) into the blends of our
cigarette products.
SCOPE GROUP RELEVANT
DEPTS APPLIED/DEVEMPMENT
XUNCTTON PRMUCTION
OBJECTIVE COST
CLUSTER PR=Cr RES/Dzv
DATE RLInIff WR--IMM : January 1969
REVIEW TZTLZ : HRMSM
RIMIEW TM : Development work has been delayed by the need
for father process dwelop=mt studies under
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- 5 -
NM AM 02
EXPLORATORY PR=CT MUM=
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.-M
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BATCo document for Province of British Columbia 14 April 1999

It= TYPE :p
SUB TYPE :3
SECLYRITT CODE
FUND= BODY
ORGANIZATION :ITL CANADA
GROUP NUMBER :501
LOCAL PROJF= NU)SM(S) :T-7099;02
PROJZCT TITLE :Evaluation of Reduced Sidestrom and
Self-Extinguishing Cigarettes.
PERSON RESPONSIBLE :MC3RnZ,C., PORTERX
V@ :1988
PROJX= DESCRIPTION The aim of this project is to provide the
ccmpany with viable methods of reducing
sidestream from C@qazqtvss, and possibly
designing cigarettes with self-extinguishing
properties. To achieve this, now papers from
suppliers will be evaluated as they becoma
available and c!qarettes designed with lower
filler weights will be made. Methods of
alleviating some of the problems with the
deliveries and subject-Iva characteristics of
the mainstream smcka which cam owa_- with
these designs will- be investiqated.
SCOPE :GROUP
DEM : APPLIED
FUNCTION :Xk;1MTnfG
OBJECTIVE :LZVELS; SOCIAL
I=XSTER 3IDZST?YM/AMBIENT
DATE PIVIEW WRITTEN January 1989
REVIEW TITLE Evaluation of Reduced Sldestr@ and
Self-Extinquishinq Cigarettes.
REVIEN TM= The previous progress report (June 1968) noted
that the next stop in thJS project Wan to act
an the results of the Focus Groups (Juno 1988)
and to concentrate an improving the -instream
smoking quality of the low sidest--*=
prototypes. Samples were manufactured in
September 1980, using a now blend
incorporating 20% DZET/63'k 1-4natI55% WM-A.
Two maAuf actu-rinq densities were specified,
19.6 and 21.6 cg/cc in order to ccmpa--* the
effect of density reduction on smoking
quality. Fuzther=ore, 3 different low
sidestram papers, all from Ecusta and
containing 35% KqO, were also tested:
1) previous papers (TOD03793) used to make
samples Sl-NS4.
2) non-el*ctzostatically perforated paper
(TOD03794).
3) now paper sill-Ission froin Xcusta
(=04016).
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BATCo document for Province of British Columbia 14 April 1999

7
The Subjective assessment by the I=, Expert
Panel Lae-cated that the newest paper from
E=sta (TCD 04016) produced a cleaner, better
tast-4nq smoke with no after or non-tobacco
tastes. No differences in the smoke quality
which may have been attributable to
differences in density or to the use of
non-&.P. paper were noted. ALL samples wore
similar in mechanics and stx-angth. Strength
was still perceived to be too low for the tar
delivery (9 mg). Based on these results. it
was apparent that TOD 04026 was the best
candidate for further development. However,
analytical analysis of these products
indicated that TOD 04016 had an unacceptably
high burn rate which resulted in cigarettes
with very Lew puff counts (z - 7.5 for 64 mm
length delivering 9.1 mg of tar) and high
sidestroam generation rates (X - 1.74 mg/min,
35-40% reduction). These results confirm the
f -':dinqs of BAT UK 6 E who have also tested
this paper.
Conversations with Icusta allowed us to
procure a modified version of TM 04016 with
an improved burn rate. This paper (TW 04298)
was received in November 1 988. samples were
made using TOD 04298 and a now recipe at two
densities, 23.8 and 21.6 cq/cc and two
ventilation levels, 0 and 30%. The now recipe
was a currout market recipe (Players Special
Bland) containing:
56.5% lamina/3.3% P-M/18% W-3114.6% light air
cured/3. 1% oriental but only 4.5% OMOD
The objective was to boost the atrenqth and
taste level of the mainstream smoke. Pron the
Subjective testing, the best sample was the
30% ventilated cigarette manufactured at 21.6
cq/cc and delivering 11.6 mg of tar and 1.00
mg of nicotine. Its taste quality was
perceived to be similar to du Haurier Light
King Size but with slightly more irritation
and impact. However, although this sample was
perceived to be the most acceptable. it was
also perceived to have an American taste which
is unacceptable for the Canadian market. The
sidestrom reduction with these samples was
dozarmined to be 40-SO%.
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BATCo document for Province of British Columbia 14 April 1999

During these tests samples war* also made in
72 M lengths Using Rcusta, paper and Om
sample was manufactured In the 84 = length
using a paper fron Ximbarly-Clark. Both the
72 = cigarettes and those cigarettes made
with the Xtuborly-Clark paper were
subjectively unacceptable. The cigarettes
made with X/C paper did not have reduced
sidestreast: a 3% reduction to a 15% increase
relative to the brand avers".
,The next stop in this project will be to
rework the blend containing 65% lminn/20%
DMT/15% NTS to incorporate some oriental
tobacco to boost the -4notram taste level
and strength while zaintaining a sid*st--*=
reduction of approximately 60%. Samples will
be made sometim in rWxmary.
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BATCo document for Province of British Columbia 14 April 1999

RECORD M-13 : P
SUB TYPE : S
SICURITT CODE
FUMING BODY
ORGANIZATION : ITL CANADA
GROUP NMMM : 5713
LOCAL PROJECT NMEM(S) : T-7711
PROJZCT T1=2 : Cigarettes Designed for Low Nutagenic Activity
PERSON REMNSIME : PORTER, A.; B=MORIA, M.
ErrCRT : i9as
PROJECT DESCRXPTION : Various opinions for the design of low
mutagenicity cigarettes will be considered and
prototype products made. Am well as testing
for Ames activity, consideration will be given
to the smoking qualities of the product and
the smoke deliveries.
SCOPE : GROUP
DEPTH : FUNDAMENTAL
FM4CTIOH : GENERAL
QBJzcrm : REGULATORY
CWST-rR : BIOLOGY
DATE REV-= WRITTMEM : Januav 1989
REVIEW TX"-I-rz : Cigarettes Designed for Low Matagenic
Activity.
REVWI( TMr. : Turther exper'ments have been carried cut
using the John Payne Tar Predictor (JPTP) as a
means of generating condensate for Ames
testing. The effect of tobacco packing
density in the combustion tube was
investigated using I g samples of Senior
Service tobac=. With increasing packing
density, condensate weight decreases and
specific mutaqenic activity increases as
follows:
Density Condensate wt. Suec.Matagwacity
(q/cc) (mg)
0.157' 253 0.52
0.225 219 0.78
0.393 198 OAS
The effect of various salts added to tobacco
on condensate activity mu also investigated
with the JPTP. Solutions of sodium nitrate,
sodium c-hlorate, sodium chloride, potassium
chlorlds magnesium chloride, potassium
carbonate and silver oxide (slurry) were
sprayed onto Senior Service tobacco to achleve
a 5% (w/w) level of addition after
conditioning. Condensates were generated from
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BATCo document for Province of British Columbia 14 April 1999

1 q samples of treated tobacco. Compared with
a water treated control, the =idLzinq agents,
sodium nitrate and sodium chlorate, reduced
specific Ames activity by about 30%.
Potassium and magnesium chlorides reduced
specific activity by about 15-20% while sodim
chloride and silver oxide caused no
significant change in activity. Potassium
carbonate, which is thought to inhibit
combustion, in=mased activity by about M.
Cellulose, pectin and wood chips have been
c=d:ust*d in the JPTP. They yielded
condensate weights of 387, 353 and 620 mq/q
material, respectively For these
nort-nitroqwmx materials the" was no
indication of Ames activity of the condensate
for levels of up to 400 4q/plate.
Various amino acids have been injected into
cigarettes and their effects an condensate
activity examined. The I-amino acids:
proLins, Al An Ine, lyzine-ECII, qlycine,
cistaine and -tryptophan, were dissolved in
water and individually injected Law du
Yauziar K. S. cigarettes. At addition levels
of 1 mg/cigarette none of these amino acids
had a significant effect an condensate
mutagerucity. Lysiae SCI, glycine and
throonine were separately in, acr ad at a level
of 16 mg/cigarette (1.9% w1w) and 60-70%
increases in the mutagenic activities of the
condensate were noted. This increase in
activity was unaffected by heating the
cigarettes at 1000C for 5 min. prior to
lighting them.
It has been well established that 6o protein
nitrogen content of tobacco is highly
correlated with its Ames =tagenic activity.
A few years ago a study at B.A.T., Southampton
showed that Stroptomyces protease treatment of
tobacco resulted in red=ed mutagenic
activity. We have employed the am* prot"se
(Type XXV) and shown that protein removal La
associated with reduced mataqenic activity,
and that buffer extraction does not result in
protein removal or reduction in =utagenicity.
We have also employed another Streptamyces
preparation (Typo XXI) which has so far shown
the highest removal of protein if treatment is
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BATCo document for Province of British Columbia 14 April 1999

carried out in a buffered suspension of
tobacco (70% removal). Mmewar, when tobacco
in extracted at 70% moLsture and at the pH of
tobacco, the Bacillus protease (Type VII) and
the fungal proteases (= and XX"I) have been
found superior to the Straptomyces preparation
(TVe ZZI). ?ha success in those bit's'
attoqpts has been encouraging and we plan to
screen many more commercially available
Proteases. We.also PI= to use other
pclymarasas, such as pectinase, in conjunction
with the protea"s, to see if this enhances
protein removal. Solvent extracted tobaccon
are also being treated with enzymes to
overcome the problem of removal of papt4des;
and amino acids, which also are precursors of
mutagenic compounds.
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BATCo -document for Province of British Columbia 14 April 1999

12 -
RECORD TYPE : P
gm TYPE : S
SECURITY CODE
FUNDING DM
ORGANIZATION ITL CANADA
GROUP NUMBER
LOCAL PROJECT MMMER(S) T-6431;S-9-5-4
PROJWr TITLE Tobacco Fractioraticr
PERSON RESPONSTETZ DE GRMPRE,Y.
EFFORT 1988
PROJECT DESCRIPTION The objective of this project is to
investigate the fmmibi]4ty of reducing the
biological activity of tobacco smoke by
tobacco solvent extraction. Tobacco samples
will be seVentially wr--actod by solvents of
increasing polarity in order to race tobacco
components which may possibly be precursors of
undesired products in cigarette smoke.
We are also exploring various lab-scale
approaches for formulating the treated tobacco
into a shoot. Tobacco flavour extracts may be
added back to the reconstituted shoot.
SCOPE GROUP
DEPTH rUNDAME!V=
FUNC=ON GENERAL
OBJECTIVE REGULIXTORY
CLUSTER BIOLCGY
DATE RZVI3K WRZTTEX : January 1989
REVIEW TITLE : Tobacco Fractionation
MIEN TEXT : We are presently exploring the feasibility of
reducing the biological activity of cigarette
smoke by solvent extraction of the tobacco.
This treatment involves the serial sexhlot
extraction of a tobacco sample by the
following sequence of solvents: petroleum
other (35-60-C), acetone, dry ethanol and
cold water followed by an hydrolysis of the
tobacco residue in 0.8% aqueous KOH. The
tobacco used for this work Is a flue- cur od
tobacco lamina from the upper aid-stalk
position ground to pass a 20 Mash screen.
After each extraction stop the weights of the
extracted materials (or dried solukklos) are
obtained after evaporation of the solvent.
The weight losses as percentages of the
initial tobacco weight are calculated on a dry
weight basis as follows:
PQ
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BATCo document for Province of British Columbia 14 April 1999

13 -
Waight Loss of Initial
solvent Tobacco Weicht
Petroleum Ether (P) 4.3
Acetone (A) 8.2
Ethanol (E) 24.4
Cold Water (W) 26.9
2-SUIXORLIAM _LK)_ - -ii .2
Tobacco Residue (R) 15.0
The John-Payne pyrolysis/c=dmistion system is
being used to burn both the extracted
materials and the tabacce -residues in air at
69M. The resulting smoke condensate is
tested fer zutaqenicity using the Am"
procedure.
41h
I. a total nitrogen content of each sample was
measured by the Kjeldahl mathod. It was found
that tI
he mutaqenic precursors are mainly in
the water and the IMR so' lubla parts of the
leaf. Compared to the unt-reated tobacco, the
tobacco residues produce condensates with
activities that increases from the Petroleum
other extraction to the water extraction.
This is probably due to concentratizq the
potential mutaqenic: precursors on t!he tobacco
residue. When the tobacco residue is
hydrolyzed, the Ames activity and the total
nitrogen content (%) decrease markedly due to
the solubilization of tobacco prot*4-%
fragments and -Irm acids into the solvent.
I've polyphonals, chloroqemic acid (M) and
T
rutL,% (R) in both the tobacco residues and the
extracted materials, were analysed by liquid
chromatography. These two polypbancls we"
efficiently removed from the tobacco residuax
by ethanol and water.
The John Payne pyrolysis/ccmbustion system was
also used to obtain tars for phone-I analysis.
These phanols (mcno and dihydrozy benzene
derivatives were recovered by noam
distillation and dater-'-ad by a cclorimstric
method. A good correlation was found between
phenols ir smoke and the at,-- of the weights of
chlorcqocic acid and ratin in the tobacco
residues (r - 0.96). Full details of the
extraction work will be given in a later
report.
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BATCo document for Province of British Columbia 14 April 1999

14 -
In order to transform the tobacco residue into
a cigarette filler, we are also investigating
various ways of producing small quantities of
a reconstituted tobacco shiest. V.* first
trials wero made using finely ground untreated
tobacco (tobacco particle size < 53 u) and
the Gerlach process.
In this process the tobacco is slurried in a
4:1 sixtars of mothylens chloride and zathanol
and various additives are used: mthyl
cellulose (16%), buiylone glycol (12%) and
ort-hochosphcric acid (O.S14).
Cigarettes were handmade, at two different
weights (0. 85 q and 0. 95 q) from sheetts
all prepared by this process.
1% a Ames mutagenic activities of the smoke
condensates of these two Gerlach cigarettes
wort compared to cigarettes made f--= an
aqueous sheet process (PM) and to the Players
check cigarette.
The specific activities of the two Gerlach
cigarettes were 45% lower than the Players
check While the PCL ctgarette had a slightly
higher activity than the Players check (8%).
The next stop is to prepare Gerlach sheets
from extracted tobacco and to evaluate the
Ames mutagenic activity of their cigarette
smoke condensate.
Other extraction schemes will also be
investigated in order to reduce the levels of
proteins an tobacco.
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BATCo document for Province of British Columbia 14 April 1999

RECORD TYPE P
so TM- S
SECURITY C=z
FUNDING DM
ORGANIZATION ITL CANADA
GROUP RUNSER 426
LOCAL PROJECT NMMER(S) : T-0111
PROJECT T= : Non Routine Analytical Service to Support
R A 0 Projects and for General Troubleshooting
PERSON RESPONSIBLE : POULIN, P. ; DMONT, J.
MORT : 1988
PROJECT DESCRIPTION Ketbods are set up and analyses are performed
to most the requests made by different
services. The spec-4tic ongoing activities
are: Analytical monitoring for Purchasing and
Technical Services, investigation of consumer
complaints, measurement of humectant levels in
tobacco and fine cuts, asses zme@ nt of the
uniformity of flavour application for Pilot
and product'-an plant, and mon-routine
exploratory work for Product Development.
SCOPE LOCAL
DEPTH SUPPORT
F
1UNCTION GENERAL
OBJECT-M SUBJECTIVE
CLUSTER METSOD D9V
DATE UEVIXW WRr-"T= January 1989
REVIEW T= Non-Routine Analytical Projects to Support R&D
Projects and for General Troubleshooting.
REEME14 TM Most of the requests require non-rcutine
analytical methods, which involve literature
searches, method development and multiple
instrumental techniques.
Since the last review (June l9ea) the
following services have been rendered.
1. Spearmint Levels Analysis:
Thirty-five. (35) samples (3 productions in the
last 6 months) and I flavour solution were
chocked to ensure that the appropriate levels
of speazzint were present. Monitoring
spearmint lov*U Ls likely to continue for
another year at the rate of one production run
every 6-8 weeks.
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BATCo document for Province of British Columbia 14 April 1999

- 16 -
2. Monitoring of Humectant Levels in_
Cyacsition Brands:
Humectant levels are monitored 3 to 4 times per
year. SI-ice the last review, 18 samples of
fine cut tobacco$ 6 ITL samples and 12
opposition, have been analysed for humectant
levels. In the last 6 months there has been no
inc=ease in the levels of glycerol and/or
propylene glycol in these Products.
3. WU= Control of Menthol Crntals for
Guali:h Plant:
Eight (a) random. samples of menthol crystals
were analysed in July 1988 by GC to detect
trace Impurities. Guelph plant receives now
sh#ments of menthol 2 or 3 times a year.
4. Consumer Comclaint Investigation:
To date 22 complaints have required chemical
analysis to investigate reported off-taste,
oily spots and/or the presence of grease in the
tcbacco. Add4itionally, there have been some
problems associated with solvent contamination,
and inappropriate menthol levels an a
Particular brand of mentholated cigarettes.
S. Levels of Plasticizers :in Cigarette
Filters.
The praceft-re for detarmininq triacatin levels
using the Miran instrmant mu reviewed in the
fall of 1988 in order to "mass the flexibility
of transferring the instrument to Guelph
Plant. At this time an alternative method was
developed to determine triac*tin by GC. In
light of the limitations found by the plants in
torms of qualified personnel and laboratory
equipment, it was decided that the Hiran
instrument would rise in R S 0. Analyzes of
filter rods will continue to be done in R & D
an a semi-routine basis, 3 or 4 times per year.
6. DetermLiation of Saccharin in Filter Tins:
This work is continuing as results indicate
that the changes in the manufacturing process
affected by the supplier La January 1968 do not
satisfy the original IM specifications for
saccharin. To date 7 samples have been
analysed.
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BATCo document for Province of British Columbia 14 April 1999

17 -
RECDRD TYPE : P
SM TY-OR : S
SECURITY CODE
TMING BODY
ORCUMMION rTL CAMA
GROUP NMMER
LOCAL PROJECT NUMBER(S) : T-905002
PROJECT Tr= : Detarminatl= of Environmental Contaminants in
PlAmt Working Area and lbrte-n-l atmosphere
PERSON RESPONSIBLI : POULIN, P. ; DMONT,J.
EFF(M : 1988
PROJECT DESCRIPTION : The concentration of environmental components
such as menthol and nicotine mey be
periodically checked to ensure that-levels in
plant atmomircheres met safety guidelines.
Monitoring of external atmosphere which may be
associated with complaints, i.e. tab Co
odou.r, may also be assessed periodically.
SCOPE : LOCAL
MM : SUPPORT
FUNCTION : PRODUCT`=
as"-ECTIVE : REGULATORY
CLUSTER : FABRIC@CICN
DART REV M WRITTEN : January 1989
PZ7j--JW Tr= : Determination of Environmental Contaminants in
Plant workirm Areas and External Atmosphere$.'
REV TM : Your analyzes of menthol in air have been done
to support the development of a mm method Ecr
the determination of menthol. These analyzes
will Continue, as time permits, until the
method is fully developed. Future work will
-at likely be sporadic and serve way as
periodic checks of the external consultant's
results.
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RECORD TYPE P
Sun TYPE S
SECURITY CODE
FUNDING BODY
ORGANIZATION I'M C LV M
GROUP NMMER : 417
LOCAL PROJZC.' NMM1R(S) : T-6967;S-9-5-2;02
PROJECT TITLE : Sidestream Smoke and -Irritation
PERSON RESPONSIBLE : DUYAW,J.; POULIN I P.
EFFORT : 1988
PROJECT DESCRIP'TION : This project will investigate the, irritant
propert-las. of sidastream cigarette zmake. The
czanical composition of the -km (vapour
phase and particulate matter) will be studied,
with special attention to known irritants.
This will be carried =t in conjunczion with
Subjective evaluation of sidestream irritation
in order to deteridne which components of
sidestrem smoke are mainly responsible for
irritation with a view to reducing their
concentration.
SCOPE GROUP
DEPTH FUNVOENTAL
FUNCTION PU=C AFFAIRS
OWECTIVE SOCIAL
CLUSTER SIDESTIREAM/MBIENT
DATE REVIEW VRIT=K : Januar7 1989
REVIEW TITLZ : Sidestram Smoke and Irritation.
REVIEW TM A simple exposure system has been set up. It
is derived from the cubicle technique
developed by BATUXX and will be used to assess
the subjective irritation of reduced
sidestrem -products. Preliminary experiments
are planned to boqim in the 2nd quarter.
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BATCo document for Province of British Columbia 14 April 1999

RECMW TYPE P
SUB TYPE S
SECURITY CODE
FUNDING BODY
O*GAKrzA=OV M. CANAr-A
GROUP NUNBER, :415
LOCAL PROJECT UMCIER(S) :T-5549;.4-3-2-3;02
PROJECT TI= :Development of Chromatographic Methods
PERSON RESPOUSnU :D13MNT,.;.
EFFORT 1988
PR== DESCRIPTION MC methods will be set up to amllyse
d4flareirt classes of compounds such as
sweeteners, carbohydrates, bmwtants,
casings, ions. The objective is to respond
more efficiently to analytical problems by
developing methods for man-volatile or heat
sensitive ccqpcmds and finding tize and cost
saving alta=atives to already ccist-Ing wet
chemistry methods.
SCOPE :GROUP
DEPTS :APPLIZD/DEVELOPMENT
FUNCT:ON :GLVDAL
OBJEC".7VE :NON
CLUSTER :NETFCD 3XV
DM REVIEW WRITTEN :January 1969
RLV=- V TITLE :Development of Gas and EL4.qh Pressure Liquid
C=cmatography Methods.
REVIXE V TM[T :Due to the requirements of Projeft Day, there
is a need to develop precise and efficient
methods for analysis of cartain ccmponents
such as : PAR, phanols. and mitagems in smoke
and proteins and polyphenols in tobacco.
These components will be quantified either by
GC or HPLC.
Ph*nols in Tobacco:
Routine analysis of polyphenols in tobacco is
easily done by HFLC. Different tobacco
fractions obtained by solvent extraction were
analyzed for chlarocianic acid, scopolatin and
rut!z levels.
The peak purity of the different polyphtnals
in the sample were confirmed with the UV
spectra obtained using a phatodiode array
detector.
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CD
BATCo document for Province of British Columbia 14 April 1999

- 2C
PAR in S=ke:
HPLC with flucrimetric detection is a very
efficient and ac=rats method for determining
polycyclic arc=ric hydrocarbons in C=Wlax
mixtures like tobacco smoke.
A Howletz.-Packa-rd fluorimetric detector (HP
1046A) has been purchased. Individual PM's
wore quantified with this detector of tor
separation of a C-18 analytical column.
Since smake contains thousands of components,
clean-up procedure must be poeformad before
qualitative and/or quantitative analysis of
the individual PAH IS can be perforjmd-. One
approach is to first isolate the PAR by semi-
preparative 57-C. which requires about 60 min.
per sample. Another approach uses
so lid-]-' C,L-.-; ad e3r--action, i.e. Sep-Pak C-18.
This method requires only 10 min. per *=WI*.
Preliminary results for the % recoveries of
the 7 PAR's analys" with the Sep-Pak
procedure are as follows: fluorww (98 + 1),
phenanthrene (94 1), anthracene (91 * 2),
fluoranthene (96 1), pyrene (102 + 3T,
h*rizo (a) a:.t@=az an* (81 t 1), benzo(i)pyrrene
(70 + 1).
Future week plants include the following:
- final -aptim4zation of the clean-up
proceduzo.
- evaluation of the cartridge capacity.
- validation of the method with NBS standard
and Cart @416 lad cigarettes.
- evaluation and/or identification of 9 other
PAS I s.
- ascertaining the peak purity with different
detectors.
BATCo document for Province of British Columbia 14 April 1999

21 -
RECORD TYPE. : P
SUB TYPE : S
SECURITY CODE
FUNDING BODY
ORGANIZATION ITL CANADA
GROUP Nunn
LOCAL PROJECT NMCM(3) T-7084;04
PROJECT TITLE Fractionation of Cigarette Smoke by HPLC and
Identification of the Different Ccqxmndx in
Smoke Fractions
PERSON RESPONSIBLE DUMOST,J.
-,PORT
23- 1988
PROJECT. DESCRIPTION Fractions obtained by a EPLC fractionation
procedure will be analysed to identify the
different compounds or class of compounds in
each on*.
SCOPE GROUP
DEPTH FUNDAMENTAL
FUNCTION PUBLIC AFFAIRS
OBJECTIVE REGULATORY
CLUSTER BICLM
DATE REM W WRITTEN January 1989
REVIEW TITLE : Fractionation of Cigarette Smoke by HPLC and
Identification of the DIfferant Compounds in
Smoke Fractions.
REVIEW TELT : The condensate of Gaulolse cigarettes wax
fractionated by HPLC, as previously described
(1), into 6 different ft-actions: 71, F2. F3,
FYI, FF2 and M .
These fractions were analysed with an BP
GC/MM cystsm, courtesy of a a W , and appear
to be composed of:
rl : mostly saturated hydrocarbons, branched
or straight chain (from C,., to C33).
r2 : phenol and its alkrl derivatives, long
chain non-armatic alcohols (saturated
or not saturated).
r3 : son axa-arents, such as quinoline,
phanylpyridins, and oxygenated compounds
e. g. casibration dial.
C)
NJ
BATCo document for Province of British Columbia 14 April 1999

- 22
M: alkaloids such as nicotine, nora.4-cotine,
mv mine, anal-ins and their faml
derivatives.
FF2: mostly nitrcgen-containinq coq3ounds,
such as 2-pyrolidinous, acetam-4ft, 2-
propenamids, nicotine, 3-pyridinol.
M: carboxylic, acids, hydrwqacids and
diacids.'
The chmical constituents of these fractions
we" also identified by GC/NS at UT
(UK A 9). This work confirms the results
found at S S W. 2he most active fract-ion, an
indicated by the Ames test was fraction M.
A report an the fractionate= procedure and
the Ames test will be issued in the ftturs.
BATCo document for Province of British Columbia 14 April 1999

- 23 -
NM AREA 03
smoxniG BEMLVZ=
(-,I
i
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BATCo document for Province of British Columbia 14 April 1999

24
RECORD TITE : P
SUB TYPE : S
SECURITY CODE
F=xNG SOOT
ORGANIZATION : ITL CANADA
GROUP NUMER : 441
LOCAL PROJECT NUMBER(S) : T-8079-1;S-11-15;03
PR=CT TrME : Program for Upgrading the Recording and
Duplicating System for Human Smoking
PERSON RESPONSTELE : BISSCNNF= M.-
EMRT : 1988
PROJECT DESCRIPTION : Ongoing activity to upgrade the recording and
duplicating system for monitoring human
smoking behaviour. The aim Is to wu=* that
all aspects of human smoking behaviour are
accurately and properly monitored.
SCOPE : GRMP
DEPTH : SUPPORT
rMxInON : GMIERAL
OBJECTrVE : SUBjEC."IVE
CLUSTER : SMMMNG BEHAVIOUR
DATE REVIEW MTTEH : January 1989
MICK TITLE : Program for Upgrading the Recording and
Duplicating System for Human Smok--g.
REVIEW MIT : Mis is an ongoing activity to upgrade the
recording and duplicating system for
monitoring human smoking behaviour.
The rebuilding of the Smoke Duplicator with
four ports has been completed.
Validation and tests with smoking machine
profiles and smokers' profiles have been
conducted to determine the reliability,
variability and precision of the results.
The now Smoke Duplicator mm in qperation at
the and of September 1988.
k report will be issued by the and of May 89.
PQ
BATCo document for Province of British Columbia 14 April 1999

25
RECOM TYPE P
SUB ME S
SECOR M- CODE
FMING SMI
ORGANIZATION ITL CAN=
GRM 4MMER 427
LO= VROJZCT NUMBER(S) :T-8079-2;S-11-15;03
PROJECT` Tr= :Review of Alternative Methods for Duplicst4-n
PERS" FIRS"SIBLE :BISSONNETTE, M.
KrIVOT z1988
PROJECT DESCRIMON :T.1-8 aim of this study is to assess altarutivo
methods to duplication of recorded profiles
for measuring mouth level smokA constit4ents.
This wi" be an ongoing activity.
Cc r. of a prototype device to measure
TP.4 by M reflectance is completed. Initially
or-* typs of cigarette will be tested to assess
the linearity of the device's response, the
reproducibil-Iti of the results and any
methodological constraints such as amount of
material required for analysis. If the
technique przves to be accurate and reliable
under standard conditions for a range of
pr--&cts, it will be assessed under human
mckinq conditions.
S=Pl-z MOUP
DUTH -zUNTJQVLNTAL
M, =ICK womwToG, PUBLIC ATFAMS
aBJECTIVE SUBZ-Z.=Y-Vz-
CLUSTER SIA USAVICUR
DAV- REVIEW WRITTEN January 1989
REVIEW TITLE Review of Alternative Methods for Duplication.
PZ7-"-,w TEXT Due to other priorities, thin project lure been
postponed until Jamm.ry 19e9. A letter will
be sent to R.R. Johnson of B S W. USA and/or
to BAT-3outhamptan for their expertise on that
matter.
Recommendations will be written for Jully 1989
about the continuation of the projects in terms
of accuracy, reliability and accuracy all
results, ease of use and cost effectiveness.
CD
BATCo document for Province of British Columbia 14 April 1999

- 26 -
RECORD TM :P
SUB TYPE :S
$EC== CODE
Franc BODY
ORGANIZATION :ITL CANADA
GROUP NUMBER :428
WM PROJECT NUNMM(S) :T-8077-17;S-11-15;03
PROJECT TITLE :The investigation of the Effect of Nicotine
rnhnnewwnt an Human Smoking Behaviour
PERSON RESPONSIZ-7 :BISSONNE= M.
EFFORT :1988
PROJECT DESCRIPTION :The aim of this study is to assess the
changes, if any, occurring in human smoking
behaviour as a result of cigarettes with
enhanced levels of nicotine. Results may
indicate whether and how smokers are
compensating for the nicotine delivery of a
cigarette.
SCOPE :GROUP -
DIM :FUNDAMENTAL
FUNCTION :MAMMTING; PMMIC AFFAIRS
OBJECTIVE :SUBJZCTTVE;LZVELS
CLUSTER :smoxnta amvion
DATE REVIEW WRITTEN :January 1989
REVIEW TITXZ The Investigation of the Effect of Nicotine
Enhancement on Muman S=king Behaviour.
REVIEW TMIT So work was carried out on this project during
the last 6 months. Duplication on a puff by
puff basis and total cigarette basis of a
representative sample of the recorded profiles
for each subject and cigarette will be
initiated in may 1989 and should be completed
by September 19189.
M)
CD
un
BATCo document for Province of British Columbia 14 April 1999

- 27 -
RECORD TYPE P
SUB TYPE
SECURITY CODE
FUND= B=
ORGANIZATION ZTL CANADA
GROUP NUMBER, "O
LOCAL PROJECT NMOD(S) : T--8077-18;S-11-15;03
PROJECT TITLE : Chemical Characterization of Whole Smoke as
Generated Under Humen Smoking Conditions
PERSON PZSPONS121-Z : 31SSONKZTTZ,X.
ZrFORT : 1988
PROJECT DISCRIXTON : To compare the net=* of hi-an generated smoke
condensate to smoke condensate generated undqr
standard conditions in order to identify
possible areas of similarity or dissimilarity
which may relate to subjectively perceived
atr---41-utes such as irritation. The
feasibility of using 0/14P analysis as a
procedure for ccuparison will be assessed
following a pilot study. consideration will
also be given to developing an artificial
mouth to simulate the humid condiltions within
a smoker.
SCOPE : GROUP
DEPTH : FUNDAMENTAL
FUNCTION : MAM TING; PUBLIC ArFAIRS
OBJECTIVE : SUBJ'1:-C--M
CLUSTER : SHMMMS BMMV70UR
DATE REVIEW WRrrM : January 1989
REVIEW TITLE : Chemical Characterization of Whole Smoke as
Generated Under Human Smoking Conditions.
REVIEW TEXT : This project has been postponed to September
1989 due to some changes in project priority
of the work program.
The literat-are review concerning C/NP analysis
and studies an human generated whole smoke
will be continued during 2989. A report
should be issued for October 1909. Depending
on the reccamondations of the report and an
the results of Project T-scai-3, consideration
will be given to whether the O/WP method is
still valuable for the continuation of the
project.
job
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BATCo document for Province of British Columbia 14 April 1999

28
RECORD TYPE P
SUB TYPE S
SECU= Cmm
FUND= awi
ORGANI=ON ITL CAN=A
GROUP NUMBER 425
Lo= PROJECT NUMBER(S) : T-BC77-20;S-11-15;03
PRCJ-T= Tr= : Investiqation of the Smoker-Product
Tntarac=4on Model
PERM RESPONSIBLE : MC3R=E,C.
V7GRT : 1988
PROM= mscRIpTzoN The obj*czive of this project is to continue
to investigate smoker-product interactions by
identifying and assessing the relative
importance of the different elesumnts involved
in smokirq. Them* elements include subjective
characteristics, objective behaviour and
product delivery. To a this objective,
information and results from: other projects
will be used as a basis for statistical
analysis. In this way, smoker-product
interactions may be studied under a variety of
ci-cumstances.
In addition, Project T-am-7 lure been
incorporated into this project. Project
T-8082-7 describes a project which examines
the sensory properties of a migaratts as it is
smoked. The duplicator will be used to smake
cigarettes to varying lengths following an
Individual's own profile. The sensory
properties at *act stagis will bo compared.
SCOPE GROUP
DOM PUNDAMERML
FUNCTION MRRIMTING; PUBLIC A7FAn. S
OBJECTIVE SUBJ-&CT'-V--
CLUSTER smoxnfG RMHAVIOUR
DATE REVIEW WRITTEN : January 1989
REVMV TITLE : Zovestigation of the Smokax-Praftca.
Interaction Model.
RZVMX TEXT : Due to changes in the priorities of the
Product Research Group, it has been decided to
delete this Project from the work program.
Any work in this area, will be ccn&=ted as
part of specific projects with the Human
Suicking Behaviour Group.
L#4
C)
BATCo document for Province of British Columbia 14 April 1999

- 29
RECORD TYPV- :P
SUB TYPE :S
SECURITY CODE
FUNDING BGDY
CPAAMI=CN :ITL CANADA
GROUP NMMM :429
LOCAL PRO=CT MOWS) T-8080-1;S-11-15;03
PROJECT TITLE Determination of the Human objective Responses
to Modified or Novel Product Designs
PZRSW ZCSPQNSI= B1SSQtQW-1T-0,X.
EFIM :-1988
PROJECT DESCAIPTION This will be an ongoing project. The work
will provide infor=tian regarding potential
advantages to the smoker asxociat*d with naval
or modified producer designs. Results my help
to determino designs which are most acceptable
to the smoker and which also fullfil-I design
criteria eq. reduced exposure to CO, or offer
enhanced sensory characteristics. This
project will also include investigations of
conventional designs which appear to have
sensory properties that are different. relative
to other products of compax-als dmLi7ariem.
SCOPE :GROUP
DEPTH :FUNDAMENTAL
FUW."TON :MARX---TING
OBJECTIVE :SUBJ-ECTIVE
CZMTER. :PRODUCT US/=
DATE RZV32V WRr."M :January 1989
REVIEW TITLZ :Determination of the Human objective Responses
to Modified or Novel Product Designs.
RZV13X TE= :This project is designed to respond to
requests from Product Development and
Exploratory Product Research. A now study was
started in July 2988. The -4n objective was
to test the hypothesis that extrame
tar/nicotine ratio cigarettes result in less
tar absorption by the smoker.
Smokers have been recorded while smoking
cigarettes with tar/nicotine ratios ranging
from 6 to 52. Duplication of the profiles
will be initiated in Ja=ary 1"9 and sbould
be issued at the end of --r 189.
j@b
BATCo document for Province of British Columbia 14 April 1999

30
RZOMM TYPE : P
SUB TYPE : S
SECURM CODE
rmna B=
ORGANIZATION M CANADA
GROUP NMfBER 426
LOCAL PROJECT NMMZR(S) T-8081-2;S-11-15;03
PROJECT TITLE Investigation of the Effect of Imagery an
Smok-4rq Behaviour
PERSON RESPONSTRrx BISSONNEM,M.
Z?MRT 1988
PROJECT DESCRIMON The aim of this study is to assess if an
interaction between smokinq behaviour and park
name and design exists which may be
conz--ibutinq to changes in subjective
evaluation. initially a =all scale internal
study will be Initiated using the MMTA. panel
and identical projects printed with various
names and placed in alternative packs. If
war-rar.ted. this project could be extended to
Include a larger number of external
.a.-tic-4pants to deternine if the addition of
imagery results in common switching trends
between acceptable and unacceptable brands.
This project is scheduled to begin in late
1988.
SCMME : GRCUP
DEPTH : FUNDAMENTAL
FUNCTION MUM."M
OBJXCTM SUBJECTIVE
CLUSTER SMCXnM BEHAVIOUR
DATE REMN WRITIM January 1989
REVIEW TT-= Investigation on the Effect of Imagerr an
Smoking Behaviour.
REVIEW TM No work was carried out on this project during
the last 6 months due to changes in the work
Program priorities. However, this project is
tentatively scheduled to begin in January
19a9.
C)
BATCo document for Province of British Columbia 14 April 1999

-402371110
110.
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0
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- 32
RECORD TYPE : P
SUB TYPE : S
SECURITY CODE
PUNDMC BODY
ORGANIZATION : ZTL CANACA
GROUP NUMBER : 443
LOCAL PROJECT NUMBER(S) : T-7710,04
PROJECT TrTLZ : Biochemical Effects of Tobacco Smoke Exposure
in Experi-onto' Animals
PERSON RESPONSIBLE : BiLn4ORIA,X.H.
MORT : 1988
PRO= DZSCR=CN : These studies will investigate so= of the
mechanisms that protect against or Once the
effect of insult dus to mainstream or ambient
tobacco =cka. It will involve measuring
glutathione, ascorbate and other protective
chemicals ard enrimes in tissues of animals
exposed to chemicals or tobacco smoke.
SCOPE : GROUP
DEPTH : FUNDAMMAL
FUNCTION : PUBLIC A:--- AMMS
OBJECTIVE : REGULATORY
CLUSTER : BIOLOGY
DATE RXV-Z-N WWI= : January 1989
REVIEW TITLE : Biochemical Effects of Tobacco Smoke Exposure
in Experi=ental Animals.
REMOF Tw. : 1) GSH Ceplotion and Resvnthosis in small
The effects of tobacco smoke an the tissue
levels of the antUXjAMtS, vitamin C and
glutathiore (GSH) in the rat and guinea pig
have been invest-4qated. Results indicate that
both the -rat and guinea pig are well protected
against depletion of GSH by tobacco smok*.
Acute exposure of these two species resulted
in only a slight decrease in the level of GSH
in tissues, followed by a rapid recovery in
6-24 hours. Based an evidence in the
literature that the hamster is particularly
sensitive to tobacco smoke, this species was
also exposed to mainstream cigarette amok*.
The results showed that the hamster is also
rolativoly ref ractile to the effects of
tobacco s=ka in terms of GSH depletion. The
effect of sidestrom smoke on hamster tissue
GSH will be examined in the near future. It
4bb
BATCo document for Province of British Columbia 14 April 1999

- 33 -
Ls also planned to study the effects of acute
and subchronic smoke exposure on diethyl
maleate or buthionine sulphox"mine depleted
animals. such studies are importart in
understanding the mechanism of tobcco smoke
toxicity.
2) "Passive" Smokinq Stu &I an
Recent epidemiological studies have generated
considerable concern about environmentall
-- ----- tobac= smoke and its toxicological
potentiall. Consequently, as part of our
McGill toxicological research proqx%sm,
methods have been developed to investigate
'passive' s=kiN.
Zn preliminary studies smoke was generated
using 1, 2, 3, 5 and 10 smouldering
cigarettes, the smoke being diluted unifo=-'y
In a small prototype chamfcor (an loan from
Bloreseazch Laboratories). Levels of TPH in
samples of this smoke drawn by the
B.A.T.-Mason inhalation machine were measured
spoctrophotcmetrically. Rats wore exposed in
the chamber to 50 and 100 puffs drawn from the
mocks generated by smouldering I or 2
cigarettes. No significant ABE induction was
observed in lung and kidney when, rats wore
exposed to 50 and 100 puffs of a 1:5 dilution
of ambient smoke generated fras 1 cigarette.
However, the" was significant AHH induction
in both theme tissues when the rats were
exposed to 100 puffs of a 1:5 dilution of
smoke generated from 2 cigarettes.
Based on these results, a larger (1 cubic
meter) environmental chamber was designed and
Constructed in the workshop of the
Pharmacology Department of McGill University.
Levels of TPH caMarablo to the pilot studies
have been generated. The next stop will be to
confirm the initial rat ABE induction data
before going an to a more detail-ad study of
passive smoke.
C)
FIQ
LW
NJ
BATCo document for Province of British Columbia 14 April 1999

- 34 -
RECORD TM p
SUB WE
SECURITY CODE
FMING BODY
ORGANIZATION TTL CAN =
GROUP NUMBER 444
MM PROJECT NU)MXR(S) : T-7708;04
PROJECT Tr= : Biological Effects of Tobacco Smoke and
Tobacco Ex,--acts in Short Term Tests
PERSON RtSPOtTSMEX : BnMIORZAA.H.
W@ . 1988
M-= monvics Ba&.orial tests will be employed to study the
mutaienicity of make condensates from IM and
opposition brands, as well as now market
antics, to ensure that ITL products rank
favourably in a comparative study. By
dettrzining the mutagenicity of smoks
condensates and fractions from different
cigarettes smoked under different conditions,
the aim is to Identify theme parameters which
affect cutaqenicity. The effect of additives
on condensate mutagenicity will also be
stood as will smokeless tobacco products.
SCOPE GRGUP
DEPTH AP-F-=/DEVMOPHMHT
FUNCTION PU3LIC ATFAMRS
OBJECTIVE REGMPMRY
=STIZR BIOLCGY
DAM REVIEW WRrr= : Ja.-.uary 1989
REVIEW TrMZ : In the last 6 months of 1988 the following
three a;;Zts of this project were dealt with:
1) Mutacenicity of Cigarettes Containing
T-Mgical Fruit Canino
Cigarette samples containing fruit extracts,
made for Project SATURN, have been, tested for
mutagimic activity. The results indicate that
trepical. fruit casings do not influence the
mutagsaicity of the condensate. Consideration
may be given to exploring differently prepared
fruit and vegetable extracts for their effect
an Cigarette woke condensate mutaqenic-Ity.
2) Scre*n-4iq of Antioxidents and other
Chemicals for Their Ability to Reduce
Smoke Condensate mutagenictty
The protective effects of sAtioxidants in vivo
has Prompted us to study the effect of
ex;osure an levels of these Chemicals in -1-1
anizals
C)
L#4
BATCo document for Province of British Columbia 14 April 1999

35
Results from in vitro studies indicate that
many anti=idants -reduce the mutaqeaicity of
amok* condensate. Recent studies have shown
that catechol, resorcin-ol and phytic acid are
not mutaqonic in the Salmonella system, but
r*d=a the =taqenicity of woke condensate,
whereas MUM and quarcatin while reducing the
mitaqanicity of condensate are mataqenic in
themselves (loss than additive). Ca schin and
uric ac:Ld did not have any effect on smoke
ntaqwdc!.ty nor were they mutaqenic. Such
studies might reveal an antilazidant with
potential for reducing -ths mutagenicity of
cigarette smoke condensate.
Of the chamicals tested in a standard
screening procedure and found to reduce the
mutagwdcity of smoke condensate, two, sodium
solonite and sugenal were applied to
flue-curod tobacco cigarettes to investigate
whether condensates of reduced biological
activity were produced. Our study shows that
application of either selenium or augenol to
tobacco does not reduce the mataqwLic activity
of the smoke condensate. Thus, the earlier
reduction of smoke condensate muzagenicity
observed in the standard screeninq Procedure
was most probably &-a to the affect of these
chemicals an the oxidative enzymes in the
liver 39 activation system. Whether animals
treated with these chemicals will produ-ce
liver extracts with a reduced capacity. to
activate smoke condensate will be investigated
in the near future.
3) MutagenicLty of sidastroom Smoke
condensates
Considerable interest exists in reducing the
outaqenicity of sidestrom smoke as well as
its visibility. An earlier study car:ri*d cut
using a single cigarette type (plain-*ad,
flue-cared) showed that both mainstream and
sidestrom *make condensates were similar in
Ames activity. This Study was extended to
include a Low tar c===4j&l brand
BATCo document for Province of British Columbia 14 April 1999

- 36
(NodaIllon), a plain-end (Chock 2a) and an
=qmwimental. low sidestrom cigarette (S-3).
The remits obtained ohm that the sidestroom
condensates frou all three cigarettes were
significantly lower than the corresponding
-Inotromis condensates. More studies Shculd
be undertaken.
4@-
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BATCo document for Province of British Columbia 14 April 1999

- 37 -
Wm ARE& as
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BATCo document for Province of British Columbia 14 April 1999

38
RECORD TYPE : P
SUB TYPE : S
SEXRM CODE
FUNDING BODY
ORGANIZATION : ITL CANADA
GROUP NUMBER : 2429
LOCAL PROJECT NMOU(S) : T-0580
PROJECT Tr= : Utilization of Biotechnology in Tobacco
Improvement
PERSON RESPONSIRTZ DUPLESSIS,G.
EFFORT 1988
PROJFcT DIScRTPTx= The objective is to develop now lines of
tobaccos with IWroved characteristics
(nicotine, resistance, chemistry) through
cellular hybridization and call culture.
The work will cover the use and
evaluation of a number of nicotiona Soo.
in hybrids with
N. tabacum. The programme is carried out
in collaboration with the Plant Research
Centre and the Delhi Research Station of
Agriculture Canada.
SCOPE : GROUP
DEPTH : FUHDANE!f=:APPL Prim
F UNICT
ZION : LEAF
OBWIECTM : AGROTICHHOLOGY
MUSTER : IZU
DATE REVIEN WRITTEN : January 1980
RZV=W TITLE : Utilization of Biotechnology in Tobacco
Imp=vement.
REMY TEXT
Incorporation of antibiotic resistance into a
wide rang* of genotypes is progressing well.
Modifications to the initiall fusion protocol
and the selection protocols have bow made.
The initial approach appeared to allow too
many non-hybrid escapes past the selection
stage. The modified higher antibiotic
concentration selection system appears to be
successful. Recovered material frm the
fusion/seltction process exhibit distinct
morphological differences. Co rif an
whether these are true hybrids shcmild be
concluded in early Sul==.
C)
N.3
LA4
'-4
--4
BATCo document for Province of British Columbia 14 April 1999

- 39
RECORD TYPE : P
SUE TYPE : S
SECURITY Cam
FUNDING 3=
ORGANIZATION : ITL CANADA
GROUP NUMMER : 414
LOCAL PROJECT NMOM(S) : 7-0575;L-23-1-2-1;05
PROJECT TITLE : Evaluation of Flue-Curod Tobacco Pesticides
PERSON RISPOMIM : DUP-IMSIS'G.
EFFORT : 1988
PROJECT DESCRIPTION : The objective is to ensure that Pesticide
usage does not affect unfavourably the quality
of Canadian grown tobacco. Samples of tobacco
from field t--W2 with candidate Pesticides
are provided to the manufacturers by the Delhi
Research Station. Samples are made into
cigarettes and subjective mck* quality is
assessed. Results of subjective tests and
agronomic data are reviewed by a joint
ccmmittee. The Canadian Pesticides Division
now -require that subjective tests be conducted
prior to licensing. Pesticides considered
are: herbicides, fungicides, insecticides,
mematicides, and plant growth regulators.
This is an caving activity.
SCOPE : GROUP
DEPTH : APPILIED/DEVELOP.MM
FUNCTION : UA7
OBJECTIVE : AGROTECHNOWGII; REGULATORY
CLUSTER : MAY
DATE REVIEW WRITTEN : January 19ag
x WM T I"TLZ : Evaluation of Flue-Cured Tobacco Pesticides.
REV=K TZXT : Evaluation of the LC formulation of
trichlarfon (Dylox) In procs*ding. Studies by
Chonq gt Si demonstrated that over 95% of
remainirg residues war* el iminxted during
curing. The Pesticides Division (AV. Canada)
still has not ruled an the registration of
set!xwldin (herbicide), nor the arthene (label
modification), and bactcqwine (Bacillus Gyp.)
insecticides.
The now proposed U.S. pesticide tolerances
have been issued by the U.S. Federal Trade
Commission. A review of available data
demonstrated that all compounds currently
registered for use an tobacco, when used
acccrdinq to the label, will leave residues,
Lf any, that are well within the proposed
tolerances.
4bb
co
BATCo document for Province of British Columbia 14 April 1999

40 -
RECORD TYPE P
SUB TYPE S
SECURITY CODE
FUNDL4G BODY
ORGANZZRTION ZTL CXQU
GROUP NUMBER
LOCAL PROJECT UMMM(S) :T-0576;05
PROJECT TZTLE :Development of Canadian Tobacco Varieties
PERSON RESPONSTATE DUPLESSIS,G.
E PPRT 1988
PROJE= DESCRIPTION The alm of I-hi- work La to enable the
production of those varieties which bast met
ITL requirements and which are beat su!-ted to
Canadian growing conditions. Brooding work is
carried out by Agriculture Canada. rz
collaboration with the CTKC, InT is Lrmlved
in testinq/evaluation of strains and in
recommending licensing.
SCOPE la=
DEPTH APPLIED
Ml CTION LEW
OaZZIC=r. AGR0V-C8NCLCG11
CLUSTER LERF
DATE REV133W WRITTEN January 1989
AM= T= Development of Canadian Tobacco Varieties
REVIEW TM So mm strains were rscc@ndsd for licensing
in 1969. Evaluation of somatic strains are
still in progress. Strain 84AT38 will again
be part. of the current evaluation as this
strain may matars/flower three to four days
earlier than current variation.
There was an L-icrease in 1958 in the growing
of U.S. developed variation that produce a
predominance of lawn styles of tobacco, as a
result of market situations in Canada in 1987.
The demand for such styles having dropped
significantly in 1968 and the efforts by
Agriculture Canada in conjunction with cosipany
representatives were such an to reverse the
trend.
CD
r%J
Q-4
%-0
BATCo document for Province of British Columbia 14 April 1999

- 41 -
RECORD TYPE :P
SUB TYPE :S
SECURITY CODE
FUNDING BODY
ORGANZZATION :ZTL CANADA
GROUP Nmon :410
LOCAL PROJECT NUMBER(S) :T-0577;'-23-1-2-5;05
PROJECT T= :Maritime Tobacco Research Programme
PERSON RESPONSIBLE :DUPLESSIS,G.
EFFORT :1988
PROJECT DESCRXPTION :The aim of this work is to improve the
useabilifty and quality of tobacco grown in the
Canadian maritime region. R G D efforts
consist primarily of evaluating the best
selections from the variety trials.
Subjective sake quality., Leaf chemistry, leaf
physical quallity and agronemic characteristics
are reviewed. The work is funded by the CTKC
and Agriculture Canada with some input from
grower associations.
SCOPEr :GROUP
DEPTH :APPLIED/DEVELC2.4ENT
FUNCTION :LEAF
OSJZCTZVZ :AGROTZCMiOLCGT
CLUSTER :LEAF
DATE REVIEW WRITTEN :January 1989
REVIEW Tr= :Maritime Tobacco Research Programme.
REVIEW TEXT :This joint goverment/industry prigz -P had
been under fire for the last three years.
Agriculture Canada made an about-face decision
and has agreed to maintain its participation
in the proqra=* for the foreseeable future.
A now series of black rcat rot resistant Lines
(IM-series) developed at Delhi has entered the
Programme. No strain has yet been found to
replace the low nicotine Islangold in Prince
Edward Island/New Brunswick.
C:)
BATCo document for Province of British Columbia 14 April 1999

4.0 2 3 7 1 1 2 1
CL
.40
E
so-
Jo-
c
E
0
0

43
RECORD TYPE : P
SUB TYPE : S
SECURITY CODE
rUMING BODY
ORGANIZATION : ITL CANADA
GROUP Nmmn : 450
14CAL PROJECT NMMER(S) : T-0"5;06
PROJEC! TrME : Redryinq Process Study GLT Aylmer
PEWS RESPONSIZ-7 : HIRTLE.K.
EFFORT : 1988
FROjECIT' DE50=10N : This is a joint p=jW. between IM Canada and
BAT (UXU) R & D. To= flue cared grades from
the 2984 crop were each processed th the
Aylmer redrier via 9 different drying
conditions. Sauples will be taken for
subsequent chmical. physical and subjective
smoking tests before and after drying (2
years). Cigarette sm*les will be made from
each sm*le for assessment by both the ITL and
BAT (UXr.Z) R G D laboratories. Tobacco
samples from each treatment will be provided
to each laboratory for physical and chemical
analysis.
SC0PW- : GROUP
D-70= : APIPLIWDEVEMPMMIT
FUNCTIOff : LEAP
OBJECTIVE : SUBJECTIVE
MUSTER : PROCESS RES/DKV
DATE REVIEN WRITTEN : January 1989
REVIEW Tr= : Redryinq Process Stzdr GLT Aylmer.
PEVVIEW TM : Smoking by cur E3qaet Panel has not indicated
any strong Preference for any of the nine
drying conditions. Color measurements to be
made by BAT (UK A E) R A 0.
M.)
N)
BATCo document for Province of British Columbia 14 April 1999

44
RECORD TYPEZ : P
SUB TYPE : S
S-ILCMITTY CrM
TwOna s=
ORGANIZATICN ITL CANADA
GROUP NUMBER 456
LOCAL PROJECT NUMBER(S) : T-5350-1;L-13,06
PROJECT TITLE : Stem Process Optimization - STS Process
PERSON RESPONSTW-7 HIRTIS, K.
WFM 190a
PROJECT DESCRIPTION To study B.A.T. Gez=yls STS (Steam@ Treated
Stem) expansion process for stem.
To evaluate STS unit in an TM plant.
To investigate the effect of all stem process
parameters in order to red=* filling value
variations and to in=sase drying capacity.
To provide now process specifications for all
plants if quality or cost improvement can be
made.
SCOPE : LOCAL
DEPTH : APPLIED/DEVELOPMENT
FMFCTION : PRODUCTION
OBJECTIVE : PHYSICAL
CAMM : PROCESS RT-S/DLrV
DATE REVINEW VP-V."-" : January 1989
REVIEW TITLE : Stem Process Optimization - STS Process.
REEVIEN : Evaluation has been delayed by equipment and
production difficulties. Test results to date
indicate that:
- Dryer input tobacco moistures , between 35
and 4S% N.C., slightly affect filling value
but not Particle size distribution.
- Tobacco shrinkage is less at 45% tobacco
input moisture than at 35%.
- Cut width did not appear to affect filling
value but did affect partial* size.
- Tobacco filling value at 150-180 cpi was
higher than at 90 cpi.
St= product, physically equivalent to our
current WTS I mm produced by reducing stem
flow, larger holes, plate and no air
injection.
other samples are planned for senscry
assessment end produt design.
BATCo document for Province of British Columbia 14 April 1999

45
RECORD TYPE : P
gm TYPE : S
SECURITY CODE
FUNDMG 3ODY
ORGWZATION : 17L CANADA
GROW NUNBER : 448
La= PROJECT NMMER(S) : T-5452-4;OG
PROMCT TITLE : The Addition of GLT 14-11- Strips to Cat
Tobacco for Subsequent Expansion by the DIET
Process
PERSON RESPONSIBLE : RnUrl=, C.
2770M 1988
PROJECT DESCRIPTION DIET tobacco pro&ced from 90% flue-c=od
strips did not show any physical, chemical and
subjective differences as compared to DIM
Produced f-m 100% flue-cured strips. It is
planned to test DIET tobacco containing 15%
and 20% small strips.
SCOPE LOCIL
DIM APPLnM/DEVELOPMENT
FUNCTXCN LM
OBJ-&C-&.,V-v COST
CLUSTER PRODUCT RES/DE7
DATE RVrM WRITTEN : Jacuary 1989
REM 9 TITLE : Addition of GLT small Strips to Cut Tobacco
for Subsequent Expansion by the DIET Process
MIEN TEM : RBE have been incorporating 10% of small
strips (through 5 meoh, over 8 mash) in DIET
recipes since the beginning of January. These
now recipes will be closely monitored in
prodaction, before any further increase is
made.
PO
BATCo document for Province of British Columbia 14 April 1999

RECORD TYPE
So TYPE
SECURITY CODE
FUNDING BODY
ORGANIZATION
GROUP NMCM
:P
:S
ITL CANADA
448
LOCAL PRO= NMMER(S) : T-5352-1
PROJECT TITLE GLT Strips Redrying
PEISON RESPONS= HxtTLI, 1.
ErFORT 2988
PROJF= DESMEPTION To datexmine the impact an tobacco temperature
of incxv"inq throughput br 50%, who*
maintaining pad depth and-pcocess, air grain
leading.
SCOPE LOCAL
DEPTH : AFPL=/DEV=PMMT
PMC210N : PRODUCTION
CBJECT11M : COST
CLUSTER : PROCESS RIS/DEV
V= REVIEW MMTTEN : January 1989
REV= TIMZ : MT Strips RedrTinq
RE973EN TM : Increased throughput is being si-lated in the
present redryer in azter to dateridne the
temperature reach" by tobac:co.
U4
Ln
BATCo document for Province of British Columbia 14 April 1999

47
Wm Am 07
TECMUQL SCMCES
L
I
L LA
BATCo document for Province of British Columbia 14 April 1999

4a -
RECOID TYPE : P
SUB TYPE : S
SECURITY Cam
rMING BODY
ORGANIZATION : ZTL CANAZA
GROUP NMMZR : 24SS
LOCAL PROJECT HMMERCS) : T-56T7
PROJECT TrTLE : Resiftal Solvants/Taint-Odour Threshold Level
Determination
PERSON RXSPOKSMM1 : DUMN, R.
zFFQRT : i9sa
PROJECT DISCUMOM : The threshold level of r'es4dual solvents in
boboard at which cigarettes be=ms tainted
are to be determined. The G.C. Ana I ysix will
be used to determine the level of residual
'solvents' (alcohols, 'eftem, resins.
plasticizers In she /slidas/blanks after
the printL,iq operation.
The Ezport Smokinq Panel will subjectively
evaluate cigarettes e3qoesd to various levels
of the solvents for taint-odour. the project.
is carried out in collaboration with
suppliers.
SCOPE : GRCUP
DEM : AP?r-r--n
FUNCTION : MARKETING
OWICTIVI : SUBJECTIVE
CLUSTER : CMIOSENSORY
DATE REVIEN WRITTEN :anuary 19S9
REVIEW Residual Solvents/Ta-tnt-odour Threshold Level
Determination
T47M 1 r 1, only Limited data Ls available to date as the
supplier involved with the project is under a
heavy workload an a result of implementation
of C-51 packaging modifications.
Preliminary results have bam obtained an
cigarettes otposed to tolueno, wthancl, ethyl
acetate and N-propyl acetate. Results suw*st
that cigarettes exposed to concentrations in
e=esa of 250 pym toluene or 400 an N-propyl
acetate W 25 ciqwettes in 500 aL jars my
impart. undesirable or modified tastes to the
c1garettes.
Work an this project is not wqxated to resume
=1-11 November 189.
Q-4
--4
BATCo document for Province of British Columbia 14 April 1999