BENCH MARKING PROCEDURES FOR APPUCATION OF GENOTO)aCrTY ASSAYS PROJECT 93.11.103 Objective - To apply standard protocols with 4 in vitro genotoxicity assays to representative cigarettes in order to establish: a. variability of response b. reproducibility of response c. effective operational control and use of the data set. The cigarettes Included in the follovving tables are candidates for inclusion in the above project. A range of commercial cigarettes have been proposed on the basis of the maximum variation that may be encountered in products requiring examination BAT brands have been selected on the basis of knowing the design features incorporated into these products. The optimum number of products that may be examined is currently considered to be 10. Consequently, 10 products (shaded) representing the maximum variation have been highlighted for inclusion. However, part of the project Is to determine if 10 is the maximum that may be examined and consideration if this may be increased. All stocks necessary to complete the study will be purchased together. Those required for the repeat study, 6 months after completion of the first. Ml be deep frozen. It is not the purpose of this study to examine variation In biological responses to smoke condensate as a result of any changes in materials available to produce a particular product at any one time. A limited series of non BAT brands are also listed for consideration of inclusion If required, though a lack of detailed design knowledge would not permit as great variation to be examined as with the BAT brands. As part of the study, a range of chemical analyses of compounds considered of appropriate by outside bodies will be carried out including. Particulate delivery Carbon monoxide pH of tar Nitrogen oxides Nicotine Hydrogen cyanide Phenols Volatile hydrocarbons Catechols Aldehydes Tobacco specific Nlrosamines C) C:) co 00 co BATCo document for Province of British Columbia 15 April 1999 BAT Brands Virginia Modified Virginia us- Style F 15mg .cured 78 Fife: d US - style Stif6 "I-1.11 9 % carbon filter 3'ik 8% amm'On' let recon. M L G 9MG! 11mg 'i GGI 10-13mg -vefifilk 625%,'@'-@@ Wnfiritlo"M:33% 50 marytand cur F i 6 FILf6,66idd, 60% tobacc 1 6 % 2 Ex 6% St 19% con. 3 E H - @M 3.9mg high mod. Firecured bran V6htilatlo@ 66.5% Ventilation 28% tom Kenya.,, ,Ofdi'dure&7'A:'@' Flue cured 20% 78% Firecured 'Stimn*, `,,it@2% Case Burley 29% tobacco. Oriental 15% Exp fc 16% Stem 20% flue cured product w amarelin.ho tobacco. 14 mg 14%dark air cured tobacco CD -MR. -sh. co co co BATCo document for Province of British Columbia 15 April 1999 BAT Brands Virginia Modified Virginia us- style B&H General Venezu Trad Belmon suave. Micro Flue cured 78 US--sty m' % carbon I Reco iA 8% arhm n recon. B&H SF'Sudan-, B&H/SEFK.,, Mary Long V y Lights 1 1 mu, 1 13ma 0- Venbladon i 50 c4id Flue 50 --tobacco. rc M. lid B&H Ultimate B&H Ultra M Nyota 3.9mg high mod. Firecur6d bridd-,@' 3mg Ventilation 28% 'from Kenya.,: Ventil Flue cured 20% 78% Firecured Flue cur., Case Burley 29% -tobacco. v NM Oriental 15% p fc 16% Ex Stern 20% Hollywood - Brazil'.. - flue cured -product with amarelinho tobacco. Boule Nationale 14 mg 14%dark air. cured tobacco 4b. C) CD CD OD 03 C7% BATCo document for Province of British Columbia 15 April 1999 NON BAT BRANDS Vlmlnia Modified Virginia US-Style DunhiU P.Stuyvesant Marlboro 15-16 mg Holland I Belgium 12-15mg 13 mg Rothmans Lights merit 9-10mg 8mg Embassy P.Stuyvesant Merit Ultra Ughts I 4-6mg 1 Extra mild 4mg AM C) co co co BATCo document for Province of British Columbia 15 April I