Disc-jtsicns or, Canadian Bi-cnlooical ---ocrarr-le
neld a,
- , :-:nerial Tobacco Lt'..,Jul. 14, 1982
Present: Cir. P. J. Dunn
Mr. P.S. Wade I.T.L.
Dr. 11. H. E i I i -qori a
Dr. T.A. Smi th
Dr. R.E. Thornton. B.A.T.
1. On account of the s-iccess of the collaborative proararrie at McGill it
was recommended that the research activitv w-.'-.- McGill continue alone
witr sclecil'ic proje--ts wnich comolement the C.-': biolooical orocramme.
7he present proura.-re should consist of the following main areas of
rese!rcn.
a the ambient smoke programme, already un:e@wa.v. and the
subject of c:,llabc-ation with Dr. EcW:non of McGill
University. This 41z funded bv C.-.V..C.
ti a procra=ie cf worp, ainted primarily at -.aentifying compounds
in smoke resoonsib;e for mutaaenic activitj*but also intended
to suoDly some information on tne rance of activity in
different products/differeqt ways of smo-Ancp% This to be an
I.T.L. study.
c', the develiona-ent o-' a micronucleus test. '_* be used in
COnjLnCt4on 6-th 'm-.;nq tissue. To be fu-:ed by C.T.r.C..
alre---dv subri:'ted and acreed.
d any necessary work to ccmolete the AHR t@jdies in preanant
animals.
In aevelopinc tne ne. a-A-!s of wori@ (tl and (c it wits aareed that the
mutaoenicitv studies should take first priorit.v-in terms of effort
allocation.
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;t wPS !:-eej at the 19RI Research Conference that there was a need for
'ncrease: understanding of the effect of product chanqes on biolooical
acltivit'.,, and of the related chEmnical comDosition/bioloqical activity
in'erfa;e.
A.tho,,.;:- Vese areas have been studied extensively over the years,
limited progress has been made. However. the utility of the Ames.
test in or-oducing a rapid assessment of mutaaenic activitv makes this
the most promising approach towards meeting the objectives listed above.
4. S:ecif-:!tiY tobacco smoke condensates Would be subjected to lim-itec
fractionition procedures and the biological activity of eardn fraction,
ano of tne wnoie smoke determined by the Ames test. The smoke condensates
w d :-c:-a-ed as follows:
a 'ro- different tobacco tyDes
4rcrn different smoking reqimes
sidestream smoke.
an: _noss4L'-1v from different products (desior oara-_-_2-s cnancet,
E.@ of the matrix of results it should be possible tc de:errine
weather r3t there are any consistent activity/fraction Datte-ns.
Suzosecuentl van attenot would be made to characterize biolonicallv
active fractions'. Hicwever, this work would, of necessity, have to be
ca-rie: :j: at Southamoton'since the necessary analytical tectnicues are
a-.="atis there-.
i7-:'iei a commitment from G; & DC towards this project. a'EtIOUC-n
ana@.-:@-_a` work would probablY not commence until mid 19.22-. Drozzatly
t-=- LIM evoe--.4se available within the biological sciencesi=qrgupwould
be used tT SeDarate comoonents of active fractions, with a vie-. tc
ana:vs,.s -..' C"Clopriate analytical procedures such as FTIR, II'S e,:.
7. Su:n a v.:uld ca-aliment the deproteinization prograC=e-_-?-::,-'sed
wl,@--'.n t-s o-ouc- whic@ would be carried out by GR DC.-
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8 Althouoh success in icantify-ina active components cannot be guaranteed
bv this ap;roach, it is felt that the chances are considerably nreater
than for the fractionation procedures of the 1960/70's (skin paintinc
techniques and inferior analytical chemistry). The duration of skin
painting (1-2 years) prevented effective response to "blind alleys".
9. It would be desirable to contact the grOUD'S mutagenicity consultant,
Prof. B.A. Bridges. for any information on the fractionation of motor
ca- exhaust condensate, an area in which he is known to be active, and
which may be relevant, in that nitrogen containing compounds of hiph
activity are re.lutedly cresen- in such condensates. The project also
im.--lies close collaDoration between Montreal and Southamoton and on-
site discussions be'weer the Darticipatinq scientists will be required.
In any event Dr. Bilircria would carry out a literature review before
s-.artinq work, since tnere a-e some published studies in this area.
IC. The fact tnat sides:rz-a7 smo'-e apoears to have similar activity to
mainstream smoke (but a verv d4fferent chemical composition) is alone
sufficient to merit fu@ther work on sidestream smoke. which will also
be studied at.Southam:ton-alt-ouah fractionation procedures are not
envisaged.
11. The possibility o-' re--:-vinQ mutagenic comnounds will depend on their
identitv- For exairDle. it is not easy to control or remove the
aromatic polycyclic hydrocarbons in smoke. If, however, mutaqenic
activity were associated wit!i N- containing comoounds the chances of
removal/control are s@,-ewhat creater.
July 16, 196".
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BATCo document for Province of Brit!Sh Columbia 19 April 1999