AW1383 `IHPERIR TOBAe[YJ'LIHI~EO RESIAAM LPgOR4Tr)RY- ~IP5RtRIR1 PHES Hlln\eENrelfY OF W\INSTRWI AN6316ESIREAn SEIOKE CDWDEN3Am Reselrch Lahorato_ry Report Bo. 165 Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 P C· ii. Fr [; C, L!BI1Afi:' D.dS. CO, ITO,, SOU~HAMr'TOIJ I 11610( &d ~u ··t ~~lli~ IKP~IP1 TOR4CLo khlcr'E~l) liel/1i NE. T·7X)( --ra / r+ I,I1, lolr " flhu, h6t~q~e~v: ci4 0~ Jdlllas;t;eny 11~I\ Sib~S;PPU~ II YIU WIHI tO 10U111 RiH)Ri 13 1110:lltn READER, rll.\sE msrtr aBiA mmr,u, AITIII 1.I~LoCe/ IO11~G~la~" ~n~4v· m IHI ItXI OllCYL1110(1 bnCb 0111( IN#IIIINO PUEK fiKU~ Wnln It a LLDID. 111 jOlg kn jO f A1Bf"l$jb ,, ~~la7*a ik I-I 3~ r ~ ~p~ Y "i" l~~ny P~ilrilly CS/i 1A Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 Research Laboratory Report No, 165 Copy No. _II IMPERIAL TOBACCO LIMITEO RESEARCH LABORATORY MONTREAL AMES MUTAGENICITY OF MAINSTREAM ANOSTDESfREAM SMOKE CONDENSATES Project No, f·7708 Author: M,H, Bilimoria Issued By: R,S, Wade OateIssued: Hay13,19R1 DISTRIBUTION: Copy No, i, Library 1A, Dr. P,J, Dunn 2. Mr. R.M. Gibb 19. Mr, M,P, Scherbak 3. Mr,R,S,Wade 20, Or,G.W.Boswali · 4. Mr, S.M, Candlish 21, Mr, L, louchard 5, Or, T,A, Smith 22, Mr, H, Roubicek 6, Mr, E, Rittershaus 23, Miss R,R,Smith 7, Mr, F, Seehofer 24, Mr, B,J, Stirlinll 9, Or, C,J,P, Oe Siqueira 25, Mr, R,L. Rice 9, Or, L,C,F, Slackrnn 26. Mr. A, Schaffer 10, Mr, A.L~ Heard 27, Mr, E,P, Gage ii. Mr. A,L, Heard J 28. Mr. P, ieblcnd 12, Or, I,U, Hughes 29, Mr, W, Tennyson 13, Or. R,A, Sanford 30, Mr, Y,J. Ross id, Or, R,d, Sanford 31, Mr, C,J, Brown 15. Mr. R,G, Nicholls · 32. Mr, C, Warren 16~ Mr, 4,G, Nichdlls 17. Or, n,H, Bilimoria Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 SU~IY Fouf lots of ~instrcam and sidestream smoke condensates were "nrbd a e~C1C nihr Pads Int rh( I·I n·imd ~~cl cig~nttcs Jrted on a prototype fnhalaeian ryrtrm laing dcveldncd at B;A.I. South~np~~ lhiS JyStQ W~1 LC Illd by ~· to rtudy Pe ~dP~ol affect of sidastrnaln IDte an slncli rodcots, ~e 5aninicl of mafnstream and ridsbc· I.~ bdautn, lg~~(8 cthanoi, were tested for mu~aganicl~ a~p elmelll $~1II~· n$l 8~8116 for the detoctlon 01'~~a ad panic oareiooocnr, bb condensates *R dnlr in ·~Cde activity, ~~l~ls 18 is tnl in ~n of carcinogenicfg is not known, This is thc ~~t rcwrt of ~utao~ie rMd$ in lidestrean ~~I *d hwna t3 ~ rcpcatcd by a * another L~n~a C~nnd in the ·8c~r r~~~ of tobacco I·h, ClibPDF - v~~fastio.soni riJno0uc;I0N Recent increase in interest by the medical profession in sidestream snnke stems from the publicaticn of Yhite and Froeb (1) indicating small airways dysfunction in nan·sakerr cimnically exposed to tobacco sroke. To this may now be added resorts by Trichopoulos et al. (2) and Hirayas (3) which show that non·sailing Hives of heavy smokers have a higher risk of developing lung cancer and that this increase is dose-related. On the other hand, a study by Garfinkei (1) did not show an increased risk of cancer among non·smoking women married m smoking husbands. However, there reports indicate a need far a thorough examination of the biological activity of sidestnam smoke, and induced us to undertake a coparative study of mainstream and sidestream smoke condensates in the Rmes test. 3.·d.T. Sauthampton is in the process of developing an inhalation system for exposing small rodents to rideltrearn smoke, While this is still in an experimental stage, it can be used to collect mainstream and sidestrean snake condensates on Cambridge filters from the same cigarettes. Conreouentlv, last July, during a visit to 8.·d.i. SPuthamoton, I brought back samples of these two types of condensates to determine if they showed differences in the Ames mutalenicity test. The results obtained are presented in this reoart. Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 METHODS RESULTS AND DISCUSSION Four lots of mainstream and sidestream smoke condensates were collected fron~ the lalne flue·eured tobacco c~lantter tnoked an~rowtype inhalation system being designed for exposing small rodents to sidestream smoke, at 8.-A,T. Southampton. The condensates on the lads were extracted with 95f; ethanol and tested for rutagenicity by the standard procedure followed in this laboratory (5,6) using Salmonelia tl,phimurius TA·98 7~ulvided by Ass (7), The mutagenicities were calculated by (i) regression analysis of log transfond data where mutagenicities are given by interceots, (ii) regression analysis of untransfonned data in which slopes indicate mutal]enicities and (iii) converting each dose point to a specific activity (number of revertants per ug of condensate), An analysis of variance and the Student's t·test were used to detemine if there was any difference in mutagenic activity be~Jeen the ~iJo condensates (8,9). From the results presented in Figure 1 and Table i, it will be seen that all three calculation procedures enable the same conclusion to be drawn, viz,, that mainstream and sidestream smoke condensata from flue-cured tobacco cigarettes are similar in tents of Ames nutaclenicity, Whether this is true in terms of animal careinogenicity is unknown at the present time. It should be pointed out that the condensates on Cambridge pads had been stored for about three months at 4oC befon testing. It remains to be seen if fresh condensates Hill show any difference. Although this is the first clear dem3nstration of ;nutagenic activity in sidestream smoke condensate, on account of the limited nature of this study, more work would be necessary before concluding that mainstream and sidestwam smoke condensates are indeed similar in nutagenicitp by the Ames test. Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 REFERENCES i, 3,R. White and H.F. Froeb, 19ew EnPI. J, Med,, 302 [19801 120, 2~ 0~ Trichopoulos, A. taiandidi, L. SDarros and a, MaClahon, Int. J, Cancer, 27 (1981) 1 ~ 3, T, Hirayrm, Br, Med, J,, 282 (19RT) 183~ 4. L. ~rfinkel, Su$itted for publication in the J, Hatl. Cancer Inst. 5. n.H. Bilimoria, fmperial Tobacco Ltd,, Researd Lab~ra$y 9epcrt No.lh2, 1981, 6, Idem, Irperial Tobac:o Itd~, ~ero dated 18 Oct, 1978, 7, B~N. Rmes, J. ~cCann and E. Yllnasaki, Plutation Res,, 31 ji975) 307. 8. ;!.A. Brownlee, Stat's;ical ihe~~ and R:hod~la9y in Science and Engineering, J, Wiley and Sons, inc,, New York, 1960~ g. R.G.O, Steel and J.9. Torrie, Principles and Procedunr of Statistics, McGraw H111 Book Co., New York, 1960, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 FIGURE I- AMESMUTAGENICITY OF MAINSTREAM AND SIDESTREAM SMOKE CONDENSATES b3 I, LL O CI) X 2 5b0 ill w d U1 O d t u Z I dl x MAIN STREAM (1 oSIDESIR~M 4 C 3 6 too 200 sao 400 500 SndKE CONDENSATE-~G PER PLATE h G P3 L G N CCI Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 -5· a (E a Ic pt OnPI c r "5 E' C *I U"clLULn tl B PI U~T 5 PI 1 C ~r E a Pa ~ COICU C " r ·r R O Lm PI C 5 N Om IO\Oa r ,Y I)I~PI N "E 0l00C05 I VIEZ~LCU ' " )II I ~ u E rC m ;PI C r 55 (5 U) E ;O P m N ur r I V O 2, ko Inr 5n 1~ Eii L OE Y I E ~n lp I u Im 0 C In n m lp ~L cn 5 Le e: d LPI U 00 0 IUU c E o ? c L CI c ic~ ~· ci ICU PII in : O '* ' N c luE 5 O\ O CI O iLO h 01 E IEl~ O VI ~n I~ a jiu e e ptvl in 3 :6 i e O~ u B c, *i% i a o u c n 5 ra \5 N U PI e ID pt LI LO ,s i 0 r O : U'e Ln r r U ~O n E 5 c a u u .r r c L 5 Eu O eE o PI In r um 5 5 ip o *d d o Pl in O no i; U U"LI Jin 05 UPI 00 OC LO u j 3 5 cu u a a I PI PI clPl rJ W L L Y C Y U FP n, I in in r: 3 e l5in ~ 3 e r o ru os o\ ro Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 G rU h O 00 0\ k C: I i I; PDF -!::!!::!!::!.f3 StlC.i: 0111