C··~ YSi T,; Ph32535 ·,. ;"; p~env·~ac~ ~. r ssii 4~11·. IKk;caP ·1~ -'~;t ~I~1:1 MPERIAt TOBhCCO CO, OF CINNU PO.1. .:J i· ~: ~s RESEMCH LbBORATORY :%: HRFReAI s: ::· .4c·~/v,'~''i~' SEMI ANltVAI REWRT ·~:f JUU- I)ECleR 1966 L~;·! ~.·Z; :W: ·C~~ a r I:; :J ·· '~ '4' ·~! * ': ·C~ ':`.·:a" =·~:~ ~-~;,? i ~ s ts 3·9 u ~ i5 vl 1 a o Fu ru u ru Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 H b D,E, 1IIIRARY D·i\,T, Co.ttd~ , SOVT~W~PTON Jb~mal I ( ~T. Cc~ Ca~ad a~ Ze~ . dob . ~eh~ ak~l Issue: hepOTI ~. 1ec Rbb Datb Rlcsived~ Cnp~ ~3, dr c.'o~ Hdader Yage JInitials Date ttL#. I--t~~ n3li r ~I RE; ·I c~bic ~hP'J)- -D zli~ ··- ~ · illk RG~t. 4~J 3, oa ~g ·~;e N i-, I I a Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 mPuNo, .,.13~,,~. IHPERIhl, TOBACCO COHpAIIY OF C~NADI ZrHtfeD BESEARFr( UBOIAO[LY FX)N1RFhl f~: 2fl"~i957 i ··- wa rmolt aipIo j Jull·$e~n~~a, 1966 ·.·ev.'r~* '*ri eolq~led by: Hieo R, B, Smith Approved by: Dr, V, C~ RuncBlw ISSD BY: Ht, R, S, Wade ~IQ Isstn: Ibreh 1, 1967 DISTRI1IBrON: Copy No, I, libnrp 16, Dr, s, J, Green 2, libtsrl i?, Dr, s, J, encn j, Hr, d, e, laporta 18, Dr, s, J. cretn 4, Wt, B, S, Lde 19, Dr, I, J, Gnen 51 Dr, V, C, Iluweklu 20, Dr, S, J, Gnm 6, Eb., S, W, Clndl~J 21, Or, I, II, etiffith ?, Ih, D, 1, Schroeder 22, Dr, J, #, Adn 8, Dr, c, u, Ioovell 23, Ilr, II, I, Iteie~lebsn 9, Hr, C, i, Coodsle 216, Ht, M, Ioherbek 10, nr, R, t, lier · 25, Hr, A, 1, B1 Lgalr 11, t, I, J, Stit~inp 26, IIisep,Ieavy 12, Or, S, J, Ctwn n, Hr, n, s, Petoi,r Dt,S,J,Gtesn 28, la8,ll,lmith ·O 14, Dr, S, J, Crcm ~, Enginaeting DepartMnl O 15, Dr, S, j, Creen ~ Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 pREPACE This report represents s aun~aa~izdion of work undertaken within the Research laboratory duri~ the period July 1 · Decenbet 31, 1966, The projects covered are coded as in the past, with a few Idd~· tiou to those profects listed in the work progranee issued on August Ist, 1966, ~cwevsr, the recent consolidation of the Btseoeh I~bor~rotg's staff and facilities under one roof has permitted certain organizational changes to be rmde, with a vim to separating, to u Pelt a degree as possible, work of a routine or auni·Poudae service vrure fros that ~are truly de· finable u research, Concurrently, it has bsen possible to reviaw the l~bora· tory's progralame to effect revision and rearrangewnt in certain areas, and to introduce~~ Tew new areas of research, Aa a eonsepuenee, the present report is the ksl which will use the arrangement of prolects originally introduced in Novamba 16, 1964, luture guutarll reports will follou the arrsngement of the revised work hogt~a issued on Harch i, 1961, ~utthe~oo~~, it is proposed to eliminate f~0P future 1uarter19 reports much of the reporting of routine work, including that involved in certain product design projects, fines this is in mat cases of do~l~~ic interest only and has already ham reported upon fully ebsmhere, ii what has, in praotice, becotpe a proliferation of paper of doubtful mh~,Sucb work will, in future, only be presented by title, in order to ~pi~piZC Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 C 10CO Cc~Ltal Anal~tel Rcse~rsh and Sctv~rr C20CO Tobacco vJluAtion e~ooa Tohncco anbSaoktRereRreh 0 1000 ~iltrJtfon l!aaaarch C50CO Phvour Xeeearsh r,9CSC Rcduet Pes~rt~ Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 C 1MX) Ccncnl In3lvtLcnl Rlsearth and Service E 1Ulj Smoke ~nJlsJi~ Lnu31 I considerable number of revisions have been ~de to the Smoke ~h~lysi~ Lnuel for the purpose of updating older methods end bringing certain laboratory procedures and lnaintenante schedules into line with requirements for the new laboratory, h new written procedure for de preparation of s~noklnq cqdp· nent end the determination of smoke tar and nlcotloe,uaing the reduced anmple siti (C U111 has slso been prepared, New menuels In shortly to be isaued to nraca of the Analytical Service and SmPke Research groups, (RIS) c lol? ~u~5~m~.s~n~~,~!m~e~. In his studies on the rnhalation of Smoke II) Dr, Nishet attrmt\lol to determine the organic aeReent of inhaled and exhaled amoke hy gas chromatoganhifal means, An evaluation and elaboration of thr above idea resulted in the proooJal to determine by gas ehromnro- graphy the RY, wilter end qlJrws phase of a single puff or of the total smoke volume of a cigarette, To accomplish the shove Roils It is oroposed to emnlov the sllee- live abilitv of a combined r,e, and flame iaizat~on detecting system to differentiate between water and the teal organic phase within partial volumes of a puff, This is explained as follows: The fnaetlvttP of the flame ionftltion detector towards water is ~lvLnqc~uSL~ used to *nure a) the total aannle phpnr, and h) the gsseous phase (after passing Lhrourh the Cambridge filter) an alternste oatdal vnl~aaes of a puff, The determination af water in whole, and TPH · free amoke is obtained with the T,C, detector on alternate pnttini volumes of the same puff in the ehnvl' manner, In turn, then, the relative neak area of a single phssc, 1~ eomnared with I standard area, indicates the concentration of this phase within a auff,or the total puff volume of I ci~arette,ns desired, (E4F) Rlf: aisbrt, Pr, M,A,, Studies on the Inhalltiofi of Smoke, l,T, Co, Interim Report, September I, 1t66, c la~l cln~sln~c~g~e~ A two-way vented slide valve haaad On the valve develoytd hY env Ilepart No, Ri~393·R) hsa been evaluated, Ilnuever, such a svatem suffers from the diaedvantage of not resembling tile hunnn smoking action, i,a,, the movement of the ciearette prior to and after PuffinR, At Present I tree smoking laehinr! is in the develoament stage vhieh, hopefully, will simulate the nndFn of a cigarette during human smoking, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 -2· 'Ihe principle of the frae smoking machine is as follows; An artificial aellth 1.9 moved to and from its seat by solenoid Retlon, RF seat sVRtem ]s based on the SuaRe-lok principle and will be constructed from Teflon to assure A proper seal, he autn- valve will be electrically activated and timed with the action of the solenoid and the lain body of the smokinl machina~ rt is honed, later, to Install m electrical valve, (silaitar to a valve produced by ~sJoadcil~al Dirlsfon of UorthinRton) which possesses the ability to r e P r a dues established prf i nro[i Irs, eit)ler directlv or indirectly, by teas of ttansducets~ (ERF~ C 1102 nle_F~lecC d Bhacco C~dieioni~nR and ~thad of Ctin(lin?, I~pecifts 1·11111 on the Sugar C4ntent of Tobacco During the period lo question a sudden decrcnee 011 noted (apprsxt~atelg Zfi sugar) in rile reducing segar levels of All hredll involved lu our monthly "Srall I~larkct Scheme" and yet tile control tobacco (l~eck;) that is incorporated into all experimental runs remained at its normal level, c]bviously, it appeared that the problem was vleeiilted with some aspect of tile procedure in wllicb be control tobacco pas not involved, The grinding of the cut tobacco and preliminary over·aibht drying at 6S'C were ehe only operations that did not apply to the control tobacco since a Il~a~le of it was taken routinely from abulk samDle and its wEar ectent yea Ixprelacd on a ve~ vcitht blsL, Coineldentllly, Cite low sugar values were noted at ,Ipproxlmneall the same time that a small IJilay mill was brought into use for grinding small nanpl.es such as market brands, pursuing tile logical r.aurse, an expsrfment was conducted to Cgm· pare the suber content of fractions 01 the pound material pmduced from the raglar and miniature Wiley milli, In addittln to this basic consideration, the effect of grinding of tobacco at substantially different moisture levels (nlr drying versus conditioning at 602, n,H, and 70"P) was incorporsterl into the design, liicotine Analyses were also made on all samples as a matter of curiosity, Some interesting observations emanated from this work but unfortunately it did not resolve the basic problem of the low sugar levels, Although the particle siaa distribution was influenced to BocPe extent by the moisture content of the ~oh~leeo at the time ~~ giading, Ihe'vc4hlcd IverPKe nlretine M.redueing sugar le~ele indientcd that the condition of the tobscc~l prior to grinding Has probably not a significant factor, gather wide variation was faald both in nicotine and reducing sugar r.oneents of the varlou fraeeilla daalt wfdl (retalned by 35, 48 and ba laesh and tllrouRh 60 meall screens), Consistent (i,e, observed for both the air dried and EDndttiOnp.d aamples) gtd probably aignificnnt differences were found between tile weighted reducing sugar averages for the te~lrar and miniature mill Jan~rcs, nw samples pound on the regular Illill O Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·3· were found to cat3in,on a I:aleulntl~ bsala,u, 11 IM,ru rlll~et· than colttnioed By ~hose ground on the linl~~llre, n~e "~nitl:!If?cC" ap?cn~ed to be In the rtlllle clirdctla~ a~ exnljin the sud(lca eLlall~! in oycxerfa~l ag~r Icvell hut: rcpresentrd lees than bal.~ III the estimated r,2ncnl reduction, An attcnpE I,, confirm the c~[aet aT the ~rindinJ dav~el on the suHlr level by an~lyz~n~ colnnn~tc! sa~lns of two eplrJ of tobacco (~luc·el·ed ~~nd ~aECn ?~Te cnl,aP·l!i ~Lf.ch bad been ground on EL~~ cnvel:uclve I~ayJ, prclvpd el, be ncy,ative, Iluse sampler will be ren?dred pril,r to JretlinS a final eoncbn~on relative to the eguj?sl.cnee of the tw~~ mllln, A r'ull re?nte on this sturly will ha ?raduc~d in the near ~IIEllre, I II ;1 The Jlllil·ulty ve~ finally erser!J to tJle preliminary ~lcyl.l niaac of ~hll prsveClurc, ibpatcatll, I:ontr~ry In I!1Ir Lllt;i:ll I'l~:ll!fV·ll: i.('r", sugar loslel werr· ra~ulein~ Ial wet oi3)lt dryL~ at GiFC, ~II cxploalttn las anI been found but thl: altllatloe lel rc!netltcd bv rcverrlaS bl ana!ylie p~ alr·dried r~aple~ and tCf. a~l!.iatLil be a slo~stun correl:tion to the data, (BJS) C 1161 Determination of N02 in Smoke The aynthesia of ~·l~droxy·cl·m~ehgl toumarin from tesorctnol and ethyl jceto~accCatc, using polypholplloric acid u the eondenalne agent, has been lucceeafully eqltrsd, Similarly, 7·hgdroxy·I,8·dLChII coumuin LI been synthesized alng t-neehll tesorcinol, A Ifncat calibration curve of ~blorbme~l~ of nitrate nitrogen, obeying Beer's law from 8·35 its, haa been obtained with f·hydtpxy· 4, tl·dimiethyl colasarin, Ihis ia hued on a procedure developed by taby b ~tton (Natllra, ~, p, 299, 1966), for the determination of nitrate nitrogen in aoila, At preeent, the method ia being adapted for the determination of the NO2 content of tobacco Imota becauae of the indicated telstion· tip between it andlbiter Although a calibration curve hued on NOZ is ja~ to be completed, tentative pyrol)sea of micro tobacco sunples have indicated that 7·hldroxy~,B·dfnrth~1 coumarin clp be employed to determine, apectrometricaily, the Hq content of wh, (PR) C 1213 bill Eb~a~uts Anaylll4fP~I C_ut_rob_accoa k put of the fine cut eobaccoe atudy a Idtbl of tobacco I~aprel were adyzcd for ~o~s~ure content by the hip blhod, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 -4· 81 r~lPlel are tlul~tcd b~le: 1133 lbiatUre CoPIIIt in t Pirl~ Ll~t Secold Week t 8~1111 11,5 14,9 player's 13.1 13,6 JPOlrlR 15,9 14,6 EKport lb,3 14,9 " Each teaule rcptsacnts an ~vsrlla of 15 ~~lc~, e 1232 ~lll~~~et h ~lie~l tlltl Thno distinct s~plcn of IC1 Vile obtained in shirt ond Ihnddrd fPn~ Bt shreddoll fon pal cat, lad a portion of this mAtlrial van I~nufa~tuta~ into )1OX PCI Elpnn~te~, Phtsiell c~llr~ts atl Ildl on the 18lct fon, the out to;m, ·~ the cf~~nttl fon, 1" comperieon rith thl louer Iatriolfc Sheet alnltb rtcri~l, dr higher t,S.I, nuterial vee: thinner, had a hi~hnt dms~rt, hlnhsr bt,~till loed, ·II I~dlr colour lr the 3heet tea; lelr lillinX, darker ealaorl and ion lipll in the cut ion; and leao finneea ·1 poorer end etahilltv la tile ci~lnttl lol~ Be ohlfoUl e·elP~iol ual drls that the hilhlt 1,J,S, Il~ltill wit of nlntfnlv poor gualfty, 'lhe alPjt~ of thi~ itcsti(~lfa van reported on huaoet 12, 1166 C Ijll The Effect of dbl~!e on bt and licolfll lleav[ta lo Irplirat vee designed to d~tla~ll the ctfell, if aolr, on the precllion and reprodooihility of tar end nieot~n6 reaulta if the eilaretee eanole lirl vee reduced to eix c~pr~ttl~liub· Ianple fron the pFl~lat eleven si~rltt~~, ClibPDF - v~~fastio.soni ·5· Dur(ng the drvalopnmt at tbe method it Wae sound not to he practical to reduce the site of the smoke trapping system, or to use acetone se I means of keeping the Washing volume iou, Uhef the tar and nlFotina results Were statistically Rnnlvlrd~ the yields from the aix·cigiltctte eamplea Were found to be slightly lowt (l,e, 0,3 aQI tar) and the v~tMnrnlapl~ variations to be slightly targer than those in the Iln~n·clgtatte sample, The diffsrences in smoke yfelda between the tvo nethoda Were not conaidered Ierge enough to matter When Weighed against the advantage of saving a mir.iansa of one hour/sample in smoking tint. In Ildditlon, the empirical nature of the hkcd tar Index as a measure of the Whole ~aDke oonetituents suggested the verv little Would be lost In adopting a nev let of nljtrs for routine and experimental cigarettes, As a result, the method uaa adapted for routine E1Ratctre smoklng,(RRS) C 1312 ~t~,Sealldardfr~,tion Exercise · Smoke hnill~l~e~o~ll~ Ihe I,T, G,, labnrtclry tea recently pnrtfci?aterl in tie first of a series of e::ereLen devoted to ~taolicdlza~fsn of smoke Bn31ySl9 methpdologyl nle average rssults obtained from 5 independent ~nking tun by rhs five calllbon~in~ lahca~ar~cs, England, Cennany, Auseratta, U,S,L and Caandsbcna~cd by tile numbers 1 to 5 respectively) With the plain·cnd go cigarettes auppllcd by eray are eabur~tcd below: llaborating lebaroeoty Average T,P,N. 1 44,2 2,98 2 40,3 2.71 3 82,4 2,16 4 40,0 2,32 5 42,8 2,81 It uauld appear that refinements mag he fieccJsar7 In these basic methods before progressing to more cqhx methods, (838) C 1324 Oetersination of &tain S~o~ by 1118 Pl~otowtm Research laboratory Report go, 92 has been issued · Oetober 3, 1966, (11816119) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 __ -b· 0 1121 Determination of rolvevelle Iromatle Ilvdtnclrhan~ ,,.,. ,~ .·~·r~···-·~l ·-···cl-· The Ftnccdl~re for the 010 determination of henx(a)pyrenr! In cigarette smoke has been developed according to ~l~e wlhod described in Crev's R b 0 laboratory Report Jo, Rn jll-R, hn strenpt to onit the TtC step wRI not Jueecnsful since etc analysis shoved peaks derived, probably, from the Rcctylated paper, The work is being continued to adaPt the 01.0 method for henz(a)yyrene determination and other polycylic aromatic hydro- carbons In cigarette smoke, (LIERF) C 1328 Oelcrmination of N02 uib diphcnylplcr)rlhydmtyl (OPPII) - '-~"""""~''" '~ I~ having noticed the rapid decolourisarion of DPPII by nitrogen dioxide, the possibility of using OPPII as a means of estimating N02 vas investigated, DPPH has a high solar extinctinR Coefficient at 515 Ipu and reacts rapidly with N02 st room Icnpetature, Since the absorotion of the reagent is proportional to its concentration over a wide range, it was felt that this could provide a means of estimating a wide range of N02 eonecnltedonl, A curve was prepared by dissolving a number of concentrations of NOZ in a benzene solution of DPPH and observing the resulting absorptions, it was found however, that the response of the reagent to increasing 1101 concentrations was sluggish, This suggested that only a fraction of the n02 reacted directly with DPPH to cause loss in colour, while a Iutdler fraction of the NOl'caetal uith Lhe degndcd ~RH, Ihis hypothesis vps epnffmrll by an attempted isolation of the products of the reaction, Fnly small amounts of DPPH.II were isolated from the complex mixture of products, (HEH) C 1333 DetLninstion of Acrolcfn in CiRancle Smoke Research laboratory Report No, 90 has been inaued, (wS) 0 1b13 Hiscellaneoua Analysis for Hutectants Attempts have been made to develop a method for the direct gas ehromatoi(raphfe determination of tobacco htuectant polyok In tobacco extracts, Several ettraetmta auch as water, alcohol and amwe were employed for the extraction of the lolyoL and it was hoped to determine the polyola directly by gas chromatolraphhcfat means on an aliluot of the extract, However, it was found that this Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ____ ·7· is not possible fot several reasons, Polyol recoveries tron water, Pwthnol and ethanol extracts (no otber alcohols were tried) have been vary poor ad vuLd frnn 509751, It vaa obaarved that the polyot recovery from a given tobacco sample vl~h oarfolu ai~cme contents varied vary drastically when the ~bove axttaetants ware usad, B r.ne~lal Ero~ a~ al~aacu*,r dx~un arrrt3 (II:I vlv. reapectivel.y) eeene to be satisfactory for IoDR PO1JoIs With the exception of gipetin, very strong caEalptio effects were a· countered with glycertn, noat likely ranltlny tron lcetone nnJI or other tobacco constituents, When a glyeerin·bcating extract wnn chroutogmpbad, A new procedure for axtud~a it beins tried with sueeenl, It ~canpt~all the use of acstona, foilowedby hcnnene to remwe waxes, ad the altimat~ evaporation of aertonel AT1 sanples were Ehro~natogtlphcd on 1 1.35 m a 0,006 m SS colwPa containing 2~ Carbouax 20 M and 10Z poiyphmyl ether (5 BLfi) on ~klopart P, (ERP) 0 1626 Dctcrmiaa~ion of _Henlholln~Carrool of lixed hchncs A carton wee prepaid by aItarnating Case (n~nthol~~ed cigarettes) and do hurict (ooo~enthoLeed cigaettes), The packages were wrapped in cellophane end eaaIed, After ons week of storage in a drying oahinet, B fatten of du huritr cigarettes was cx· ~mined for presence of mthol on ciI(arettes, cellophane, card· board and foil, The ~oalpos for aaCbol were negative, (HS) C 1826 Elentl~~tin~L~~Yels_9 Cana_dian ~tl!olo~cd grands with respect to tha periodic rapneat for smnthol malgla by the ~hrketing Depatnent,fonr Canadian brands of cigarettes (dne I,T, Co, ad three opposition brands) were analysed for their levsls of nenehoratoon( A tabulation of the results, comparing i,T, k,,'s representative brand to the opposition brands has been presented In a emnorandnn to 14rkating Department dated September 21, 1966, (ERP) 0 1426 haehol AnaInsis of CkaFeBca Coded 12 and t6 Cigaettes coded 12 ad 16 were aa~ncd Tot their ~nLnehar levels by the CIC Retbod, (~SIERF) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·8· c 1121 r,e,B.,e, colllboDtiv~ Stu~n.a~blhol Rteglnltl~g Three mentholated eigeretta tob~ceo filler ~~qle~ were received and Illllyzed for their ~an~hol content according to specified nrthoC, ka~rePuE~nrahie ~hod ~ln~imlcl tbnthor is quantitatively determined in ciguette filler by das chromatography of an ethanol extract, ba aoetbof iblcmll Itlpdard is used to Ilnizo crtort cased by foJtru· mental variations, Ethanol extracts of cigarette filler aamples i, 2 and 3 were chromatDgtaphed en 15' x Llb steinleol steel cot~, eoneaiain~ 10X silicons oil D,e, · 500 on ChrOlOlPlb u ~ecotd~aff to specified conditions, The menthol eoPetntneion was calculated via the ratio factor of the unknown menthol peak to the internal atanderd unetholpealt. All aaPples were analyzed In quadruplicate, Coiori~eerie ~th9d Frinc~p~l ~nthot is separated from tobacco by distillation and determined colotifri#lly after reaction with O~dimcchylan~ino· benzaldehyde, Dlatilleta diquoca of tob~cco,~np~es i, 2 and 3 were reacted vith pdinrthylunin~brpr~ld,hyda as specified, ~he pcremlage of menthol was determined by measuring the absorbance at 550 ~ lad eq~t~lw againat a standard calibration curve, All ~~lv were aaalytld in quadruplicate, Rclurtl: Ihr blloviny! is I cqari~on of the aPlqe ineer· laboratory results of fifteen participants with out ~v~nbe reeultsl Sample 1 Sample 2 S~le 3 C,C, G,C, tolar~m, Imperial Tobacco Company ltd, 0,274 0,325 0,311 0, lIZ 1 0,151 In~cr·labot~cor]r participants ,210 0,260 0,325 0,132 0,150 10,163 (E3rl\lcl Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 -9· C 1128 Development Pf~ St~ll Method Lot d~e Routine l~nthol Po~e~n~n~tion on Foil Zhs Colot~Pcer~e (reaction of menthol with e·dtm~chyla~niao· benzaldahyde) end Gas Ghtpleategraphic methoas were found to b( suitable and both were intat·eq~tod, The mC method seens to be better and is reco~acfidad for routine menthol detarminalfon on foil, A ~Pmon~dl has been ~JIIULd~ A new ktogtaph gas Ehrwtognph, model A 90·P3, equipped with an O·lmv, 5 inch Leeds and kehrup recorder as set up and Standardized according to the manufacturer's ~psctfleation, Be in~~r~wot at purehssed for the Technical Service Department for the determination of menthol on cigarette foils, ke~rd~ngly, eolu~ and menthol standards were prepsrod and the instrument vat ~tlad~~dized for menthol determinations, Tl~e repraduciblli~p of the instrument wes established with 99'1 confidence for lesrml runs of standardel (ERP) C 1433 Miscellsneous Analvli4 for_preaer?retiyes a gee FhTOYeOBtPphiC method for the determination of preeer· vatives is in the development Irate, (ERF) e Islt Miscellaneous ~q~t~S?o~t~ Tests h sinRre Pair-Coapstison SmokifiR Test #s conducted by a 15 memher, siolle-e~parettl Panel between Export (Plain) and Flayer's Ytld, The results Indicated that Flayer's had more taste andcu note irritating (903 lever of confidence), A series of four psir-(:omnarison Smoking Tests were conducted by 14 members of the sinqle-efeetette smoking Fanej between three dulYillltiCt samples with different filter tows, fhe twR did not seem La have 13nv effect on taste pualft? or overall yrl[crence, the 3.3112,nm lov sppLated ~D dcllv~r i~te ~allte than the other two tovR considered, (C~I~) ~t~9~1~e~4S~ Nfne nair-compaofson smoking tests were conducted,on a stnde- efnaro~te kola,betra~fi two K-035 levels and one t·362 level of frllvourinl! on Old Chlrm Fine Gut Tobaecol made into eieorrtles, Tuo flaowrtnff procedures were used, The results indicated no difference between flavoutinr Procedure (fRbl vs fBCtOtY)I nle use Of g'13f 81 I RuhltltUte for K-]ht was considered beneficial, the hlRhet level beffie Ftefetred, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 I· · __ _ ·10· e·a, Soooto ~(~II lac ooit-oorootloon tr~t~ tla eldlctod r " lintll-eirmltl bolio botvltn cilltl~rc~ laofooturod ta rlqet'~ Pina Cut labscco deh CI at I ad ttostld in old at nov dt~a~, Tho rccultl vm at ccnclncivc dul cnnl(;st th~l W is rmnorinr to 1, ad thlt tho old drior it laytior to Ibe IIv, 11 I1I Imle Scrlcs: llcroutcl Rtbol ~ac p~ir·ea~~rll ~ti~t IltP vr~l conduetcO rm ~he oookooo-balo eilcatte )·o1 blhr C·lo ad b flm~und m~hal I~~~, lhc lccnltl indiectll intronc dillika ~or Lbe rclll~r I·tlpl~tl· (e14 cnl I ~nhnla Ilr be r~~plnil~·tlaprl( mrbol laph, e art ealluoll Cilll Sl~k bltl LII Rclvrdoto rl to Ploa~ A sinplc onfr-eDenlliaon tmt on thc a~~ll·t~*r buis rc- ~~lcd on nr~ll ptofctolloo fat to Ploana (912 1I~1 of eontidmc3, lhc rcsnlts km lccn rCPOrtld to thl ~rktlinq li~i~ir, n!rtP~tite 11 tl ProCeto I canbinltion Ilrcrl, ad Psir-onopcrison tilt vll cldleetl on 1 I~I1I eillr bnlil, I cicnlIicanr (1~X l~ml oI eonfidcnec) diffareace #I dctletld bJ ebr doo·ttio tlat, 11 r~ir-caP~riloa dcec lcvcclcl thl I~for difilnnc~ u dnc ol hcrshncls, tl Plodurta bahl 1I11 bmb, thofn va no tleilic·r )n8aael, Pc rcllltl ·rt rtrortrd to the l~etiP~ Ifrioia, e all ~~ccllroll ypl Idlcea s~bl tllta e 291 Slal, gltill hi PLI~.C~I~I· snnkinl tilt III eallctllb rbe tntcrnni li~( yell belrla dV X-53 ad ligoid 1-372 tl~~P~n~ ~~ticltial to lornst 1 Strsn pipe tnbceco, No cillniIinnnt di!tcrcnecs sa e Irol ~r& t ~3 Sonl~ lolldlt( IrLI of Inlcrtuptiooc in LLa rsguisr lad81 rallt~ of tllo tob~eeo ha du ptoll2llont citDltel brrdl eollaetod Prdr eooosd bt nethodologl ad beolPll~l~t pro~l~·, tho b~etlol of aollooI hoo tooootll boon I1LiP~ead, In nddition to qllt~ll eL cIt* wlt·l II dr 4 ~~lc~ lics· tino J nduci~ rule! cnnllocs hffe bccn cqle~ld on tlr In~lne eollse~ld dltkl tht monthJ of Jooo to IAld~r t~el~l~l, h ClibPDF - v~~fastio.soni ·II· h IMlmry ol the first tao months of sampling is provided bell~; where tile t$ulated values Ire averages of single analyses plr· formed on each of three packages selected st r~nLln from a earton of each brand colrectad weekly during the month (I,e, the figure represents the average of 12 independent analyses), Sampling Playca IBclvedct~ "F' 'J XIS, i Mild S, LSI P, February 1,Sb 1,64 1157 1,50 ~eh 1,61 1,71 1.67 1,53 April 1,58 1166 1,63 1.51 1,58 1,73 1,67 1155 June 1,51 l,br 1,59 1112 July 1,46 · r,B 1,114 August 1,SO 1166 1162 1154 Saptsmlar 1151 1163 1,66 1,64 October 1150 1173 1,11 1153 i, 2, 1 1,62 a ReduciFg Suga February 20,5 20.0 21,3 2~~1 ~~ch 20,0 19,8 21,0 20,1 ~11 19,6 19,6 2011 19,9 ~y 20.2 20,2 21,2 19,9 June 2113 1919 2118 21.3 July 10,3 21,0 21.6 liugust 2114 2010 2214 2013 Saetember 1J,b 18,9 20,8 1919 October 2017 lf15 19,6 1919 ,3 15 19,1 It should be noted that the erroneous sugar d~t~ which were reported for the 14y samples in the previous quarterly report, have been replaced in the above alfllspary by more valid figures, Tna Min observations that can be made from the survey data are (1) the tendency of the Canadian manufacturers maJor brands to converge from the standpoint of nieatisa 6 reduting sugar lawla, (2) the unexpectedly low nonrh·co·lantbvat~otion In the data prior to the November sampling ~ciod, it ia'also noteworthy that the Playcr'~ brand (~oalL~s~l~ loua,r in 1961) L baina chlllenged by Bathoan'l a tha luuasZ nLoth brand moag Ebil ~joi group. Ihrao complete cycles (i,e, nine months of as~l~Pk) of all brands involved in the "l·hjot Market 9cheme" have noHbeen completed, Again in this case repeat analyses of the May samples have provinerl Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 __ r ·tZ· more valid data than that reportad In the previous gulrtdy report, Sines tha data collected from thll scheme !a reported regularly by the Puality Control #ftion of the Laboratory, it will nor be rapeated herein, The Ilmant cbaarvetion that 1,T, Co, brandl are nov Iplch art colnparable to the opposition In leaf compoaitlon' (I,e, nicotine end reducing suaar) then they were in recent years hi also evolved tron thil mon complete but hw extensive stvcy, · (IJS) C 1601 Iloutiu tobacco and Sa~o~e AnlYlu Tuaaeytbne oppoaition and fifteen I,T, Co, end eubaidiery bnrd~ pn Imlytld Ior ter end nleath IOnl(at in lnoke, (IaS) C t'f12 RiahCt~lpo ncetate tow Colorimetric tar and nicotine efficiency drtlnimtionl were made on a aample of unplaaticiaed 3,2 dder ecetpte roda wnu· bftutad by an experimental procael, Thia proceea L reputed to Increase the number of ctfnpa, and t8cnby increllse the efficiency, vttbout any increeee in the rod preeaure drop, Thew ptelirhry raaulte did not indicate any difference in efficiency from our regular 3,2 denier pleaticited tow, (19) 0 l'llj 3·,3 DenierlOillmelt Cetlulon Acetate T~ fin eampiee of teIlurcse uctale rode wera aeleaeed for thck filtration efficiency for tar, three of thaee ampleee, frcn Chearll, h~~ s. ellghtly hf$hr dmirrlfiLnenl (i,a, j,j), sPd lover total denier (4~ · k~,000) tbn the pcewte currently in u# byi,T, Co, (3,2 dlf Iltj,ooo t,d,), n, enull improvement In crimp number did not shou any cn~nccd filtration over tLt of the tallula tow, the traits obtained for tvO p~pfU from Ellt* man of 3,3 denier tow did not differ etatiatierily from the ~,2 dlf 16,CCK) t,d, control, (BI1S) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·1J. TP~h~P~V~L~I e 2318 Hone ~h4~1U t~FgPhYlierl Pr~Pcrti eLoi EtdaLboP ~he ~ P~rfu t~~urcd tebveo_C~oP Reeearcb laboratory laport lto,9e leaned Deoenber 12, 1966, The 1963 gradee of atripa wore evaluated by teeting blonde of #vcnl gradee of the ea# leaf typo, IPdividu~l grade ~nluu for eeverel ch~e~l end phleiced propertiee were calculated according to the Pethod outlined In RLlelteh tbontorl Report Ho, t~. The ~grternt between cbedal data for eingla gndel at ettipe vetaul data for Ilrbund end of nddn rqlcl wee not II good for the 1963 crop aa for other crape when eirgle ladu of etrfpl uere teated, Reducing eugare wore higher and nicotine levele lower than In previou crepa and th~~ waa reflected in lower levele of nicotine in the ~~Ptl, (CWR) C 2119 ~n~srio llulrCured Cradcl 1964 e~ap (811~5~ The Ri 164 Icriu of cxpeti~nrsl cigarettee hnve been cvaluntcd for ~moh tar and nieotine content, A wmotandu~ taa been ielued, (RRS) C 21110 urlaY), CrBdCI 1963 Crop The 31 155 Seti~~ of cigaretter capti~~ag linglr burlev Bt~dls of the 1961 crop were evaluated for sm~h tar and nicotine dLlivaias. 6 mcmorandua~ hae been Ialusd, (RBS) C 11111 Butler Cndss · 196LCroP.18(1·156 9srkl) Chloride and poCilli~ml detoroinotiona on air gr~del ~to~o the 1964 burley crop (RL·156) gown both on aaOdy and clay loll Ihnld no consLcaat relaeionlhjp betvua the level of either EoneEituent and dr 6Pc, The data have bsmtomaCd to the kd gvaloetion i fkLoo Croon, (RfS) Burlev Cmdcl 1964 ero~· Smqk~ ~oa_lr~h_ The RI 156 Seriea of cigatceru cap~~liny( the 1964 Butlcy Crop 8tadll gown on I~ndlr and clay sons have bean IV'11UPtld for IlkC tar and nicotine, ~ mefiPaodum heo hen iaauad, (PRS) G 2111) bnario Iluc·Clld oradaa 1965 eroo (RI 181) The above aoriea of tucn~y·ona grade Ilnptee have been anallaed k saDk~ tar and nieodnl yieldl, b m~monnlun hi been ilr~d, (RRS) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·16· CtPb ~r~·caa Prld~~(~Bp) · ~i~h$l~ litot~ Id todaolas Illlf I111I1I a I~ ettiP 114111 Oi flBC- cared Oredee tron the 1)65 crop collected et Illut IC#l e 1111 eWler p~tr~n to thet obteiaed tot the Iroad redrler eaplee tron thle crop (t,), bl. PJ, pi, 15)1 Oathebeeieot the E~· plete Init the crq would he clreliled 11 e Klderetelr ailh IlcotLI · in t~lrtll eelet crop, ~I dete heve heen teleted to the Ld Eveluetion a tleilol GtPq, (1JI) 0 211111 behr Co_otr_ol_Xreetnente ~ rhe a 1~ letiel at c~ptlttll rlotlctrrld Iron loboeoo trented uith lueklr poolh inhlbieorl hive been eoelyred for tlr, nieotinr lad phlaoll 1ele11 ip laker I rra~r her LeIO flllld, (119) ~ree Iddil~·~l p~ir-eql~l· enakiall tcl~l were cad~etld bJ the lintll·c~l~attl rainl pall, ~I nloll~ (lininpted di~· orepaoies Idlil~ tron the sri~~~l ~ob ad were fncorporeted io Dt, E,Y, 9o3well'e report, (Y) c tlllS llurlev Gr.edes 1 11(1 019 · I,~, &, 103 i 10C(ll·lll Ilrkd Niootfae ad chloride altm$~li~~ Lal beep coapletod op the Itrlp Iql~l (lblepde) tron the 191t hurler crop, I an Iredlpl Irltlllll ~t~Oiltll with thie crop which aekee it difficult to conpue ~II detl with ptaLII )le'tle fillte'l hurlev 8ad~_Zsl~~ 1')65 CBP ·(rq~~ hdr~ lour eonplel, Iron three It~lt politlonl, hnve been Innlyled, L ~ot~s~ ~I bill 1IIII(, (aO) 0 Otlll ~Jgl Llleld Yrro ?nd I,r, g, CII(C-tll la~~l Simples at 019 orllialtill tron the 1966 ad 1165 crept ad oac identified LI '611er'I e)g' Ltlq eoa~il~t~d for frorpor~~ioP into a qlt~al~l ptle tobeceo reolpeWere IPbjecled to Oielti* ead rtdle~$ ruler enelyeel, Tee hilh okotfnl ead in rager Il~b for the 19(5 Ilte 6, 010 end the"Deelere" laplel would elpeee thet the) are eoqolsl at a Llth proportion of tfp Iredee, Iieotinr 1Pd reduciag 1911 COltltl 0( 1100 ad Il~~tllyctkllll ohteiued for the 1~~ crop CII ~11 e Mre rQrl~~ illPiboLioo but etill te6dlaD trrd the rplet Iredee ~a ehe pleat, (1~) ClibPDF - v~~fastio.soni U· C 91118 I%lwrlnhPf~onaL nvc.Curld~triPLfrPUhe 1966 Crps I1,P. 111) Twenty trades of flul·cur~d tobscco strips sre being collected from the 1966 crop for the reutins determination of 1Letc#bllity, filling power, rate of burn, nicotine sud redwng ~ugln of the tobacco end the tar and nicotine levels of ~oo~c, Iigbteen of these 8gda~ bsve been tested in previous years, but two newly designated grades, DC·P and QC·2, sre to be included In thin project, (98) C 2155 Bvaluatfon of ILpin versus Burlhv to~ 1 (81! lla) 'T& evaluation of ILrwin, s new tobacco variety, has bean completed. Sa~king cLtacteristiel ware similar to Burlag to; 1 in all rellpcelll, the only difference occuring in the led nicotine of Iarvtn, which was 1~ lower than flurleg No, i, This difference was appatmt In its lower smoke nicotine yield, (929) e 2156 lue_rured Vnrictjcs and_gtraine 1~4 The RI 1Sj Seriel of cigarettes manufactured with vatietlel end ltraine of the 19~ crop were analyzed for tar and nicotine oontcnr, ~ memorandum hat been issued, (BBS) C 2!510 Va~iae~cs end S_E~o~sls etom~~DcUli Ptpext~mSntaf ~arm_(1965 Cr~ t,P, No, 100 · RI·189 Series IL~:ine aad'tedactng sugar analysts have beePmfflptetedd on tile eleven ssmples of single varieties end strains provided by the Dethi. E;merimental Farm from their 1965 crop, little variation wee noted in the nicotine content of the Eonrteial varieties or Delcrert Jelcceionl but the reducing augar contents displayed considerable variation, The data Ilwe been given to tile Tobacco Evaluation & ILison Ctoup, (BJS) C ?2145 ~liscellP~cous L1PeeJ (Lcd and Other ~bectkll) Total nitrogen determinaticnalHere Ipade on five subssmples from a hatch of eigcrette Con~Dcnenn P,elTI·treleed paper filter rods received for eKperimental putposesl The average polycthylenclina content (lllbn) was found to be reasonahlg close to the ~pacificld loading of 121;, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·16· Nicotine and total oUatoid analyses were performed on sampler of both esnadisn ad V,S,A, manufactured Old Pore cigarillos lir.h were subdivided into their basic coapllnents, Considerably hilher ~l~loid levels were noted in the Canadian wrapper ad binder relative to the American type but the Tiller components exhibited only minor differences, Consequently, since the filer repreaellt: approximately BOX of the cigar, the t~o types, on a composite basis, would not differ appreciably in respect to the slhlofn content, Nicotine and lt20 analyses were performed on ~~les of the blend of burlay Grades used in the Pioobac recipe, Zhe basic purpose aE the exercise was Co compare the grade combination from tobacco produced on clay soil exclusively, to that grown on aaotly soil, Consistent with our knowledge of the effect of soil type on hanlillr, quality and correlation of the latter with Foea$ sonccn5 tbs KZI) content of the recipe grown on clay soil was determined to be approrj.nately UI lover than found in the recipe produced from grades gown on sandy soil, Of lesser Ilgnftkmee from a rlualiti· standpoint the former recipe (I,e, clay soil) contained appx~,:inPtr)y 151lnore nicotine, ne qllpar content of triplicate samples of invert wear solutions from two additional batches in use st the frinby Plane have recently been determined, in all cases, tile results were vir!lin the manufacturer's specifications but more witllin·baeeh variation was noted than observed with the lint two toes checked, further work in this quality Control area will be confined to the teattog of a single batch at quarterly intervals, nte remaining four suqtal involved in the Sir ~sleer Erlcigh pipe tobacco aging study, corresponding to aging period: of five anti six months, have finally been analyzed for reducing suE~r EollorJin~ a delay caused by ~ueaasly3er and other procedural difficulties, The a~~ga contents of the last group of sansplen were E(lund to be within the Fage follnd for the numerous previolls semptcR that IIPd beau a6ed under vacuum for lesser periods of time, CPnffc~~cntl.y it may be concluded that fermentation does not ~prlea to be tc· aponslble for an observed change in aroma tb~t Javelopl~on ~e~ndln? with Sir Valar HAlaiqh tobacco, (BJS) C 22145 Hisallancous k_Rf~l4na~y~ih licollna and reducing sugar analyses conducted on samples of tobacco that had been visibly contaminaced during gtnuinb,on an Ontario farm adflcmt to a paving material plant,!adieatad liqnl· fieanr. cl!enlical differences from a control saglel 1IlsCer nicotlncl ad lover sugar levels were observed in the contePfneecd eobncco, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 _ __ ·If· Sil~llielntll different 1e1e1I of ~e~PeQ aota were I~tccled between two ~~II~ of ICL rd I Iia(le 1~41~ of IPt dllch ~lr, 1Dllflld for the ~fllel Ind Iroec~~l~l Ctoap, Ie 1 pirr Pt · aelalin '~II of the 'tine cut" tobIeea *rtlt nicotine Ind redncinl Ir(~ InelYIeI were conducted on nunIrouI IInplII of four coaKrciel brIndI (Bloliat two I,1, Co, br·d~Splrll, llayalll ~nd lnbaaaI) fDMltld oVat 1 b YICt plriOd In bddl pouchnI ·a 119 pound ttll, lhe air obtiole d1IEipctlop in the oheluicIl CDSOlltbP Of tbole bf·dl ii the ~rttarl) bilh loXlr content of the Ileter'I recipe uhichwIe found to be Ipptoxe~rtlt 502 L4Clr thin tba oelltl a the poSI I ilonorendUn IE~llll~ the eel will be Iept to rechnicll Blniea Bp~rerPe in the Pllr future, Hlcotloc and radueioI lular copcantl of 1,1~ ond I~OX raJpoctivaly for bt fobaeco fra c~~oll~ Ill~lr 'lippad cigalllll vo~d etl~~~tl it IIItlpicIl Virlinie recipe, To lupplaome the Inoke ~~1~I1I deEI I nicotine ~onlent of 1,4~t riI obtlined for the tobeoco frol ~r~em I~11I11 (Sola I~f~) cil~tottll, LaCeln~ ~~l~t ~nlll~cl on Cbaek I and Pick a rob~eear rcvl~ld thee the litter contlinl epproxilletely 11 nore ~uqlt which is ~op- ~i~tan~ with ita hllher ~~utlibr~~ ~i~turl contonr, Rl~ein~ and ~e~l auIor ~111III on aMploo of burial rlr~l ~d;~ wrn caald virh iart~d ra t~lr (roIolar proeaaa) aod I~u~ invurt lu~~t at ~be Wlnileon Fl~oe ravaaled B~ cluivoiant ~maunr~ of LoLI1 Mla lsarl bclpg ~pl~ad by thl two p~Pcol,c~ but dn iwltlioo of the tew ~ulr wII leell then IW coplltc, (IJS) C 22141 1965 C~p · Onttrio Flua·Curld Crldoa .~~~~~ ~I, 4,:) Tile collection of repreIentItivc IraPd ~~lls of Oot~fo Plus·curld nrldll were collected Ie uiuel It the Uelhl ptInt ~~m~~· out the )roat~llll of tla 1966 ctqe in 1965 the number of Irldal Iqlld wee inerll~sd Irol 2~ to 29 by the iddltlon of grldal PS·3, 11"-3, 1~-3, end CF-2G, nle cdqllte Ierle.l HiI ~ubjcc~cd to ni~!~b~ end 5ugnr onjlylc~ ee well es in nllultion of their E~lll~;lpn· FI~ll hot I chlllfll collPoIition Itrdpplnt, II~PI ~IC dltl Ibtllad for I few prallocPt Cllal which hive been LPI11111 con. ~~~~aell blr IIPf pll LI II ildQ, it *IAd ~PI11 tll;t ~Z I)ai crop wee LiZhrt In nicotine ~~~p the a lmplediItelp prheed~ol eropl but not II h~Bh In tile 1962 crop, SCR~luly, ~ld~uph tile Ienerll lp3~ level Se lower then Lhlt which yr~lll~d 9id~ ~a It~ Ind 1963 cropl, St Se ant Ii low 1e the 1962 crop Ir~r~~n (i,e, index corrected to conpeneete for 1 eh~lte in r~lodalep b~~ern then 1Dd the Iteecnt), I fnrthcomilSQ lIborItPry report on ~$s topic vlll ~1~1 ~~lud~ a ~~curliun of L~I Ifl~P1 cl~~it) ~3~, ICJS) ClibPDF - v~~fastio.soni ·18· C 12141 1965 Cron · Ce~rio Ills·Brd B~d,r · bb~$l~ ~I~ ~ In ~dlnric~l llmpllng of the Ilol~~cd Lelr(o (tedel unl ra~cr· teken durinK the procleatng of the 1965 crop nt die ~hner pl~~, Ie only difference in die eurolinl r(l~ bl~cl the plate wee Ihet e Irllhinat collection of e few 8redel vee conducted durilc: the fltet couple at pstltt( ueekl It the $Irr lllnt (1,1, lo, P4! Theee lumlee were forwarded ptoqtlt to the Lbor~eoV for 1 r~p~l PICO~~ end redllcinl ~ut'r ovaluetioa the rcllltl of vhld~ ~trr reletrd to the llef )Oflrl to be Pled eI e guide in dr I~rcCI~~ of ~~( crop, PI letial of Itedee defined by t,p, No, 91 were tile eene 1! ee collected et Llbi but in the c~le~ whore the PLIC 1II coilecred eI e pert of t,P, bl 94 the n~l proe~~l~ng elelon w~s not repreeented by the finel (1I1I IIP, No, 95) eelrple, Nicotine end reducing ~rl~ ·nlylse on thil Ierfel e8nln indtteted dut dlI 1961 crop vee hilh~t in nicotine ad lower in reducing lal~r that either the 1963 or 1961 crope but did not lchieve the record ~1~1 nicotine level ~se~blllhcd in 1912, ln'eJdition to 1 eo~latu prllcnt~eiln of the chenicel aetl, the Iotthednl report on ehi~ eubjeet will deel with the filling cepecity ~llun~ton, e tnl~ !JcekiY Olri~~ioo In COr;Doeition of pour PlUe'CUred C~ldOl Itom ~Y No, 9() par the lecood aoneeeUtiVe Icor eraalee of fOlf Pt~Plle 1191- P, D·t, I, j·2 hat been collected r~s~lt throughout be Iroccllill of the 1165 crop et the 411r Il~lt, They were lllel)rted er d~s ad of the lleeeon es I group for ~fzntLw ad reducing anger In the hope tbee velid eetimetee of ftw ~s~~·te· week verietion in theea conetituepts will be ab~~~d end thet tllcv q be reeeonebly cOPlietent frOl J1~·tO·~~ tot 1 IPIclflc Itlh, if thfe idoneioa een be eXtrePoleCed to e l)ogBheed 11I1I it eboPla prove to be of cool~d~S1~ III 1n experinentel blatdll ~nd product Illfb operetione, Ilrhaolh the all~th~l ~t \lee hrcl cn~lltod, the dnen Ln~ not bcn~ rav~~d ~~ yet ~~I c~~oloe~ely PI CC ape iA a pglibOP EO CcCect d~l ICiltl'lC eP ;Ilt Pcl~ eurrey, (1~) C 11152 Irlcz Gredee_ (114~ Grown on Ilpdt soil · 1965 Crop~(l,~, RDI ii."' ~h, X20, C1, tetnl nltrot~n, nicotine ad totel elkeloldl m~llao~ were plrfond on EOI~ Iqlll of blonde Of bnrley ~laal grown oil I1Pdl IOL~~ 1965, (LII~ bl 1~)1 J~ll I~III were ~lqllCeCI tron E1IJ I0il,bre,lael nMet of the Rredee oril(inetinlt on cle! Icil were delcipea for I~ort~tiol tlleir m~~ll~~~ wee tegerdel ee a· neceaeerJ,and hence tbet were not lubnitted by the Llf ~)lttMI~~ I new dlltel of colbilling Iurlet Bredes eI blonde wee iPieietcd in 1965 with the net effect th~E ~~~ chl~ic~l detn cDnnot be readily eoqLld wf~~ preoloue crop reellte, in eny event the dete have been faPY to the Ibleeo IrlllleLo end ti~i~o~ Group who uil' endeevour to glJ:h 1·I 1De(~rltltiOP upon dill (UI) ClibPDF - v~~fastio.soni -19' c 11151 ~i~t~,neo.f,~4~,~!~t-~p~?se~a. ~ ~I~I~, ~ in order to eliminate some PI the many steps that ate now involved in the handling of cigar tobacco as It moves from the farm, through the eoopetatfve' and finally to tile cigar aRnufaetut- ing plant, some different methods of packing were tried by the leaf Department on the 1965 crop, These Inpet~lenlnl packings includedl (1) blhole leaf straight-laid in case (2) Tangled loose leaf packed in casts (3) Tangled loose leaf packed in hollsheads (8) Strips packed in hogsseads (I) Pracut whole leaf packed in hollbeads These samples have been through one natural summer sweat and cigars (Rt 219 and 219 series) are being manufactured from (r), (1), (0) and (I) to determine if the methods of packing influence the smoking quality of the tobacco, (CIJH) C 22154 1966 flue-Cured Grades of Tobacco From the End of the. Redriers (eround Simples) because of the anticifatad use of the IBH computer for preparing recipe formulations, etc,, it will be necessary to have ealytical data on all grades of tobacco that are used in our prduets, Consequently, it has been decided to begin the acguisltion of such data by sampling all grades of Ilua·cured tobacco that are being, packed in our Ontario leaf Plants for domestic uar, (eCTB) Flue-Cured ~~t~l~?_l~l~rZ~.1~P~6_eS~E- L~P' No·.lll Representative samples of eleven grades from the 1966 flue-cured crop collected at hylmet (l,r, No, 113) from the beginning of the season to December Rth, 1966 have been analysed for nicotine and reducing sugar in accordance with the early sampling program introduced during the 1966·6J processing season, Based on this limited sunreg it would appear that the 1')56 crop may he very similar In chemical composition to thc·prceadinl one and aJ such it weld be classified a a moderately high nicotine - low reducing sugar etop~ The data have been forwarded to the tc~f Department, (L15) C 2257 1')60 Experimental Coop ~r_on_E~5s_~e~!om_~!~e~!~ql,,s~t~?, R1 179 and RL 1RO Samples Scrb -I~?t~e~ir~a~d_!!~qr~S~ 30 pait·eompdlcn smoking tests were conducted by the sinpk- cigar smoking panel comparing Yarteties ed Hybrids, The results have been presented to Dr~ C,W, Bwvsll for reporting. This completes the 1964 experiments, (QC) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·20· Fmoki~g Test 3elaltl on Clear Tiller Toboceo_ft·an the exPcrimel~l~ perm, f'dl~o~Ption ·1761 laboratory Beport No, 51·1 issued October le, 1?66, Cigar tiller tobacco aamplea from experiments involving fcrtllltrr tcsq cultural practices, curing methods, rind variety tests at the l'a~lonption Experimental yarm were evsluRted by subfectlvr smoking tests, The following briefly outliner the treatment in each fertilizer uperimant that received the best rating. r,llcium and Potash experiment ' · 5·8·16 fertfltcr with JCL: Ihn, eao. Sulphur and phosphorua " · 5·u·11 fcrtfliter with 12~ Rulpbt, Sulphur and potash " No definite preference, in the "Topping and Isrvbatfng" Llpeiincnt there we some i~n· diarlon that panelists preferred the sample topped at early bloom the mast and the sample topped st the bud Itaac the leasl, j6.w) in the curing experi9cnt, tobacco cured in the normal manner or with Let and forced ventilation appeared to be cqudll? AFcapDble and pratetred over the tobacco cured without heat end forced ventilation, RH 211 and i~ll purpose varieties van preferred over Itrsin ?'15 1~ I slightly significant level, in all comparlsona involving strain 105 no significant levels of preference were reached ,1It(II.!IF;JI strain 105 was always preferred by a nwJority of paneliats, C 2218 Burla'r'am Suntcr - 1163 gpp~l,P, No,_911 Total alhla~d analyses on dr 1(10 san~lLn which comprised ~II· second phase (i,el air cured) of the 19bS crop burley ~lna aur~y were collmleted during this pertod, This date combined with tIII! retulta of cl, K20 and aah ~nlpll have been slven to the Isal:ro Evaluation and liaLon Group, Nicotine analyses on these ro~nlllai have been deferred, (RJS) c 2259 ~i~_~x~e_~ln~n~l~_~_r~?n_f~a_l~l~?s~7~n~ _E?c~e~e_~!nn~!~t~J~1_S~l~~~g~ _Rt l?p~And Rt~2OO Ssnple_S~ 20 pdr·cqarilon smoking tests vetr conducted by the sinqle- clear JlotinR panel comparint IampleJ Provn with nvstematic tteabnts of ro~nah, Cnlelual Sulphur, and Tholphnn(, fhe results have I~ern forwarded to ni~ C,U, Boavall for rcfortlnr, (EAC] Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·21- 0 P~b lou·nl* Yull*~l~ · lpL! · 195/, ~~ la ~~ ~8 II A con~nw~m of thl Lurlly fnrn lurvly uhich vIA bllun L l0h9 ooneIrninll tho chlorinl ond PotIoAiun Ln~ in the Ioll And pLn~ 50 Ilaplro of burlol toblceo lwvo b(ln eolllct~~ h Ont~rlo Ind torvordod to thn Illwrrh LLOtllofy f01 ehtnicAl nn~lpl~ ond burn IlItl, ~(~1 11~111 . four (IodI, oottPrA, loot md tipl) tronIArh torn · uore all~chd ira 9 Ln oil ~~ch of gtlvc~, Ilnd, Ind oily loll t~~~, (~9) Ippralrt~l~ 1~0 1141~( of butlly tohceo Llte been rlclived bt tar l~~clreh Lbo~tcrl fat tbr IIprrint involving aifflr~PI rltll of pltlll~ flttilill lppliutioD to oily loill, hla~n tour I~~l~l(l~d~ euettr~ Illf lnd tlpl) werl collctcd fton cAeh trlluent Ad rcpllc~tln, Ilbu~ Ihw Ilru uor( otiglP~lly 11*1~1( in tbll Ilptrl·a4 tL ~~ea I 1110 i~a only won conlidlud luitlble for ~lqllnll (9rs Alril · Lne 1966 PrPlr~l~ report), (Q1) Ti" ohlprldl eoPlent of thI ngntn yund~ I~lcl n~nnt~~ ~a~ ~~ 15 f~~ oomprilfog the 196C burley i~m eurvey hac bcr eoglceb? 40 AnIlYn(n on tLo1( II Iqlol lrl CLlld bl;JlObLIYUb dy fllll PllotomeEor but lre prallptlt bllnl var~ld on, roil doeA vlll toe be fenl~dld to Chi Ib~cco Rlll~llol 11 Ll~iloa Crolp rpcll Itch ~~ u the work on dll eorrllp·ding eored ~ql~aohich 110 now in our po~lst~~on,lll bell coqlctad, (IS) e 2jto lo~C1Qltl IrlP1 1~11 Satl r~r led IICOtlPI I~lldl lrl (I1Utld oil Pill L11 1~ I fl~tCI ci~lrlm~~ (AtCO), elrralll lXlro S(zl Ad enV)I A Kiol Slre flltlr cig~r~ttl~, tiltlr tip Itficilnefl~ vlre lllo det(nnined on the I~~t two I~~1~1, hli ~II rllultl indientld t~t thl eillrrtta ~d I low prl~~urr drel (~~~ Ind Lit Ind Picattal y1o161 in the hilh·yilld ctal (l&j And 210 41cllt,), Cltrolll t~m li~l (IC i ~) fileer ci90rlttll ~lt~ clolled An b!tnl in thl upplr port of tho mdiul Idllvlry ~pl~~ ~leotlnl ~1~ ttl p~l hieh reiItlvIIP tho tIr ~dl (tLI ~1 2,2 11~4d but thl Llf Inllylio for nicodn, Ihovod it to ho t7piell of CIMdllD Pirtlnln blendl, The filter tip i1 of Iw tlr ~Ifleinnry (2~), ClibPDF - v~~fastio.soni ·22· Reaulta indicated that the Cnan I 11,5, cigarette ia a ~uitr high tar and nicotine producer, Calculationa auggeat that there is 3 tobacco bland difference between the regular and Ring Slee cigArelt(a, Thia lue nwltld in IJa )rielda approximately 251 in rxcnl of a klin~e Ring 9kl cigarette wherul L~Lr regular haa juet a favourable edge whin compared to Craven A regular, filter efficiencies ara of the aar order aaa 3,2 daler tow, but the 15;t~p leela to have an unv#lly high preaaure drop of 2,5", C 2115 filtration Sfficianciee of Standard eelluloee Acetate Bnda The lacltiglion lata iou tar and aicotine efficiency n~ultl from atandard acetate rode hae ban concluded, gaaulta from the indirect rmt~d of ctfkiacp determination confirned thole by the direct athod and Lhw elllaillltld the POallbilltY Of Mn' linearity of de opticsl daoaftp · conetntmtioo ·:clatlcnahip, It nov Iame apparent that the difrrrcnct~ are occuring due to the use of the bellws typa achina rather than the falling uater eottnrm, The ration for thia it not clear, but L thought to be due, poealbly, to puff profile ditflnnell. since It is our Ln· tention to change to the hellova type machine 3 new Iet of empirfe:ll values, for the acetate tova in current uae, haa been vtabllshrd from the acc~auLted data. A memorandum hae been Lsuld, (RRSJ L2'1~ eong~Lon o~ b~ ,ad Tm navr lit l,r, ca. ~3 oolol!tlon Ir~n~l An investigation into the T~H veraua snell tar yield ratioe haa ban completed on one sample of 111 the r,T, Co, and bppolition ~rands included in the IPM; achedured nulrket aurvov All the eigarettea, whether plain or filter lipped oere reduced to A 60mn length by cutting off the tip andlot tobacco and all vera laked to a 15nw tobacco butt length, A preliminary eaeeaement of the reaulta did not thov gross differences batveen T,T, Co, and Oppoaition bnndl but did show progreaaive ratio increaeea as the vaight difference (betveen TPH and Snail tar) increased for each brsnd, It Ls intended to repeat this experiment on the aame baPdl to obtain T~ lela m3ilture vllual and, hopefully, tJ get are meaningful reaulte uhieh uill reflect any dlffareneea in organic VolatileI In the anoke from raapoctiva tobaecoa, (RRS) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 __._ ·23· C 2317 Condensate Fowaeiofi in Clears The EotFleioo of a droplet of amoke condensate at the mouth orill~r of a cigar which finds its way into the a~P~lth while amlring hn been a reasonably comnon complaint from clxar amokera in reeellt years, h effort L being made to determine tile cause of the eondaaatF problem, Sl~e Be freguePqof occurrence appears to have ~n· creased since the introduction of the piercing operation in cigar manufacture the effect dr piercing seemed to be a lolticB parameter to invaatigate, it was also hit that cigar shape might be a ~ien!· Ikdnr factor in producing the undesirable condensation effect, lienee for testing, both pierced and unpierced cigars with four bnaL shapes were obtained (buu of lords Panatelas, Hrrgueriee Coron~~~::, White ~1 invincible, Old Port Straights), Zhe relocation of tie laboratory interrupted this work after approximately 23 uE ~he pierced type of Laeh brand had been s~hd with a bellows, constnnt volume YcllinP It the rate of two 35 nl, puffslminute, nle only brand with v$fcll the condensation aitllatidn was datecterl was Old port and even then only with an incidence of 81 (2 occurrences in 25j se wori~ is about to be resumed with the smoking 1II the unpierccd Old Pore brand, At lep.st two different mou~h·cnd arcrs I;I11 br! c·· posed during the s~okin; of the unpierced types, Thr! cusee cellrsr thar will be pursued in the remaining stages of the project. bls still to be decided, A report will be issued ou tile comnletio~l si the cr.oerincot:l work, (Jj) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·24 · ~30C~ fObFFO Ud Jltr LIIIITCh e a:2 lillinR ~,~el~~ ~r c Ille~pl of I11~11 kh~~ old the ereitr of BLleto to Ielilt Coloreesion ~ a iniliel ~pprosch to eluefdete the meobeniom of fillinl e~p~eit), eI rneeenred by the celtrfflte or cq~tjls Iroee~sel, fillinC cepeeity iI conlldered ee e function of bult~P~ vo~Pa end ~~~ ability of e eabeceo to reelsC c~ralnion, for tohaca, In ~ho aaund ian, i lurdnecc f~clor ~ltlP ~,~~,~tld JI I laenns of cvcluetine tho letter propartt, lhie r~~io ney be deflnod ee: H~r~lls Ple~~r klunl Iftlr ~s~rlnl~ i ~ volna before co3r~l~~m Vol where V end Y era filling cnpeeity end k~kln~ rssplctivaltl I oI Ip~~fp( volume the Ipp~raot voluae of the tobecro before co~· prc~llon, normally exprll~sd on ~ unlt vli~ht b~lln, iJ nultlllie~ by ~ tlcta~ oI 1 in the (hnvc rlletionshio linec lillhl clplc~y in thil 1Jot~tor) 1e reported on e 2 0, belil, ly u~s of thia flctor, it ie ehovn Chnt the lilltn~ c~pncirl propatrlel of ground eahcceo ccn be better described ~~~ ther dif[ersncls cln he abservcd ttlet would not b, dlltingul~h~d hy Illliny eq~cit) al~lrtrPt~ Ilonc, Pc f111in5 cepeciticl of tobecfPe in tile srt end Iround fon ntc eq~tsd end Lt LI chovn thlt the fillinrr gp~eltl of cut d~ecP is ditectly trpnOtelonll to~hs bulk~nn vola ot lhe I~ lob~eeo n thl_t~llla form It the la hlr(PIII IletR end le, tharlf~tc, direeel) proportionel to the effective volua, ~141118 r[ cut elblcfo to rcliet eo~rsllino le ~h~ to be Ilna~rlt r~!~r~l but not d1rectl9 proportfonel to her(inees Ilctor, (Sse Ras~~rdl leborltory Report No, 67, e 3!!1 Hlrdns~s Fletor~ ~nd lulkl~ Vol~~ oI Flu,~ur~l robnceoa Pfve eenedien flue-onrcd tobleeoe taptalentlnE luJ, r.utter, Ic~I ad tip Ir~dll, were eubjected to a I,!,i, tr~~~nt ct lmblent tc~crleuras end compered to the untreeted tobeecla, ln etl ~n~o~, bulklal volurnes were increaeed lilalfic·~ll ad herdnese f~e~ora were elilhtlt reduced LI the treetllent, It ct therefore be concluded thet tile Iein In fillinl c~ncf~t wee dependent on In increese In b~l~~t vol~l which grin wee nlightlt dler~~lcd bt i raducti~n in the ~bilig of tile tpbca I~ rcsllt c~rll~L~, ClibPDF - v~~fastio.soni ·25· The data also indicate that hardnasa factor or stiffness product is directly dependent upon, and filling capacity only indirectly dependent upon moisture content, Sae Icsereh tdb0r3t0tY IICPOt! NO~ 71~ (RW(J C 3112 Effects of Cisantte eircunferenca and Vclaht on~~t_a~t~ Firn~ese Uotalorv flepotr So, IP I,vas issued AuRus~ 9, 1966, incat- poratinll the results of this invcstidion. Hultiple linear ReRreani~ma are aresented aJ a close approximation to the true effects of circumference and night on firmneaa, These rel(reaatans enable the calculation of relative finalsn on a more realistic hesis, (CS] C 5221 Duration af_Burn and ehedal eempoaition of thL1P65 Cml of Da~tic aPd_l~ottld Shade Ynppet Tebaecl, fdborator) Peporr do, rO·t ivued'slptasbet 28, 1966, Abola 18 Ifrea of cigar wrapper tobacco were grown under ahade at the imperial Tobacco Company farm in 19651 ·'lbtrr varieties i, H, and 196 were produced, an the whole, the production was con· aidered a definite auccesa, bur to properties of the leaf which could be improved are the fin holding capacity and the color of the ash, Also, the rgnellm content Of the domestic wrapper was low coepared to that of the imported wrapper, in order to effect i~Pprolnmmb the fatilizct for the 1966 crop contained mote potassium and ~Mgnrsl~, (~I) CiKar ~anoer Evaluation · lobZ Gron (I,P, No,~02~ Sa~n~!ee representing tile first priming of VPr~tfcl 1J1 end i. Gnl::ll on the Stcwatt EarJ in Cnnatctleut were received in tl;e Lah~raal·r deer nueh of tile uorl; iud hctn eo~lcnd on dt bulk of th~ Ibad~· Crown saqlcs, C1, C20, 1~0 and total alkaloid analyses were rclllF on these sampler and the data were forwarded to the Tobacco Evale?tial and liztaoa Group with IlgO resulta for the coqleta series, (aJs") ej22q ettlet of eas and ~C1 an Cigrel~r sate of ayrn (LI 191) A pbaw of this experiment, carried out by the Analytical Section, comprised the effect of PQI end el addition to Illpinn an the Ilac~a yields of ear and nicotine, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 e ·26· The noted effects an total Imake gi~ld~ were that rat and nicotine contents were reduecd, rncreaaed rate at burn also lovered the pll[l numbers, The effect of rJbltilu~iag low nicotine 1eval CRS and PC1 In 1 100i hmiru blmd vee to reduce nicotine diaproPortioeetelY to tar, me calculated per cent change In Inoh nicotine yieldlpuft tne sppraxh~,ly the additive effect of the per cent difference I~, puft number from the control and the prr cent difference in tobacco nicotine, saPks yields vlLhlne particulAr oombinaticn (f~oa 10 · 40~) nppclr to be virtually independent of the wy in which the eanbinationa are effected i,e, 2r4 ~CZllog e2s or 20~ asllo~ 221,, l~a Ilrplt cuff n~mbtr ehanp oceun uhm 10~ of either pC1 or. eRS is added to lominl, with pCt lowering the puff number to a greeter extent than any e~apanbrt per cent eddition of CRS. b *menndam liea been iaaued, (RRS) G j2210 Ihe eftcct of CuSshoch on Clnrrrt~a pete of Burn (R1, 1o2) Smcke evaluations on experimental Figatcttu manufactured with tobetco cut at WJ, 50 and 6C c,p,f, (rheoreticel) end varying vefghrd Indkled 1) ~all proportional dacreeeae in total amoke yields with the drop in pllf[ num~cr for fixed velght ad inenallng cuts per Inch; 2) for a fixed E,P,i, and increaeing weight an upward trend in puff number hut, ealentielly, no Inercnsa in tar end nicotine levels; j) in ge*rsl,:l greater effect on preaeure drop by changing cigarette weight than by altering cute per inch, puff n~~ber decreaae ie allied to the noted increase in burn rate and thle would appenr to hve influenced lower amoke yisldl more than anr l~lr inctale in filtration by tobacco cut to finer dimenaionl, Incraaeed tobacco rod filtration II tFought to be the factor influPnclllg cbservstion (2), 1 ~cwNndL1P hea been Lacd. (1IRS) I;icot!nc! anC reducing sugar analyses were rouducted oh tt~ee rynn~l·: from the Rt·192 series of experimental plain enli I~Jrcr:rs wlll;il were produced to evaluate the effect DE eutsIIDEII an cigarette rat!! of burn anti smoking prf~er~iea, Since a ningle recipe :las used Ib~oubhaul the eerier Ilvea not coaaldercd ncecslary to anll?se all nine s/qles involved, The three samples dull with were selected from the twee lub·samples of the Initial batch thnt were created during tile processing in the !~lmt and repoelented tk ~ho~r cutslkril categories involvPd, Excellent agun~nt was nolel hch'~cll the three a~ptrs with respect to redllCIF~ sugar, Ilu~ ?tubaC!y greater lh rvlt~dl witilin·rec~ye vaxfeeio!~ was found in the rLnI:;r~ levels ~thie:l may have been introduced at the CRS blending pl~ase of the proecsslng, (~S) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·27· C j2211 h effort of Cit~un_fenner o~CiB~ltre Rate pi pur? [a Il)j) Tht effect of varying the circumference (211 · 2~nn) and tobacco wtillhr (1000· 1200 eslofst,) on lai~ ~idda sbaued 1) Snt Is circumference increases, the number of puffs and the pressure drop decresse for cigarettes of fixed veightal 2) ~Lt far a fixed eitculpf~taee snd changing weight, the converse II true; 3) os oblavtd ptevlounly in rhL Bt 192 series, no eppreeieble difference in Im~e yields as noted as puff aanbcta increased for fixed circumference and increasing cigarette weight, rhe effect of (I) was to increase smoke yields by approximately 10C/mo, seemingly, ~I the result of the large pressure drop reduction i,e, (l,b"lmo) end increased rate of burn, Observation (j) was Iltrtibured to the linear burn rate decrease end labneco rod [il~ation rather riun mi tfteet o~ presule drop change (approxiw tely ,4" 150 pg weight increase), A merrorandoo has been issued, (RRS) ~_Pe~l~cJ ·RI 191_Scrtcl Zhree sanples frrm the RI-193 series of experimental plain enll cigarettes nandectured with a portion of de same operation aE tobacco that was used in the "eutslfn~h" project (lll·t92) were aubJefited to nicotine and reducing sugar analyses, In this E4JC the variation In the sugar data was considered to be greater than anticipated while the nicotine reeults are more typical of I,urn~l recipe variebilily, (BJS) e j2212 I;ia~r~biapetr Burni?gpudllties snd_ehdc~l Co~Deslr_lpn_,~L.L.Ic~;Y~ rhe cigar sippet tobacco which was grown during the past ~wnur with additional levels of psgnesiun was cured in the normal manner, Saqres have been forwerded to the gesesrch fsboratory for ehlptcll enslysis and born rests vhilccaapmble *4le, will he put through the nowl wrapper fermentation to detet· mine if tobsceo which received high IsvaL of wgnesiup atsnds up satisfactorily during farmentation for use as a cigar wrapper, (Le April · june 1966 Progre~l Ilcport), (PII) C 3232 ~tp~tlncneal l:i!!arette Recipep Produce_d~uith Treate_d PCI and SfiS lnl·zos scrie~ Nicotine and reducing sugar analysts were performed on a series of eqeri~ata! cigarette recipes produced with NsC1·ereRtort PCI Pndlor CRS at normal lavets,lll an EqedlenC to modify rate ~I~ bent, Ibe dab have brcn relayed to the Ptl)sies nnJ Procelsin:: Cmu?, Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·28· J·b hllflll · H 206 SCtill the ooal levllo of kl nldlor PS in e V~~in~ b~Pd v,re Ire~rd rith ~ by VIICt of 1CI to dal~rinl the dflet on the ehsnknl end Phyeioel c~r~ctlri~t~c~ of th~ cflltette, The dtra ~vl been fotsrdel to the mtliet ~ld rrocclelnl Clo~ for Ivnluntion, (nnd Six plir·co~nr~~on le~t~ vere conducted by d~e ~in~l~-eianretta Jlokinlt pvlel, Rllulls iadiclte I hl~ficijl effect of NeC1 trlatment to CIS, hut I dler~snt~l effect of NS1 treotment to PCI, (6Ae) Eff,~t~~~C~A~_F~~~P~!,!?5~"!~. it ~~nltc~.L~4~?!1 0 3311 Ic~ll~ in 51x edditionll p~ir-coPpariaon tllfa were Eonducred on thi~ OMIo\e ~arlll, ~~e tc~altl of Ihlll ad patior tcltl hire not been II1B ·11Jlld, hut lpoeor iacaeloli·, h Hnrklt ReJeerch axpltialnt hen him o~~~illd to invc~tlEJH the effect of Pa a r innnediete end extended ·e bllis. ~ls vill he mlcn~! b! Fehruery to be tollnd by o oerellsl Itudt ,on CRS, (CAS) 0 332( Iffectl of dY Mditioa of llitrlte end litriel on gmoke C09011tlOP 6 lil~ltfOP in Ir~rchi~l for on exPteaetion for the reported ectiel of noJiwn nitrate in I~CI)II~PI the e~ri~llielg of smoke fton ci(arettel tre~tcd r~th lodir pittlee, it vee th0plht 10 eqrl the le~lan of 11~3 ~nd N1~~ liaee both Illtl on d~eaqosltfPn 1P~te nioic oxide end oxYgenl The cil1r0rcll uere mrt~ctPn1 fret rlPr~~~a toblceo Iprgsd with voter lolol~pnl of NeN03 end ·d I~uil~rllsd It tO'), end LOI1.B PI Production of wee d~tctl~rd end reelltl ~I ehom in the toll·- iPI ~1L: Ciglrette ~t(~)plrloc 100 ci Oontroi 2,0 4,0X NeNoj 1,0 llQ IIDj ,I 6,1X 1112 ,1 Pa Jas rsloltl eoggeJt the BllsiPI: ClibPDF - v~~fastio.soni ·_ ·19 · '2hltthe oxidadon of smoke by oxygen, probably, it not the axptl~· tfon for the action of tedium nitrate in reducing the bmz(a)pyese content in amoke, The Sr NaN03 and 6,4r NeEOZ addition would liberate egual uoounte of nittic oxide and different amounts of oxygen (nltrate liberates three times mote than nfttite) but similar amounts of bcm(s)pytens are produced by both treatments, Since the decomposition of lPaNO3 is a tso stage reaction the possibility Is not excluded that only the oxygen supplied by the decomposition of NINOZ may have an influence on the yield of bcnt(a)pgran~, There is aleo the possibility that the reduced deliveries could be due to the nitrlc oxide (which ocl.ura in egual amounts in the decomposition of Nam)j and NaNO,) reacting vith the amoke condensate, Since bcn2(3pyrenr and other p~lyeyel~e compounds are thought to (I arise through Irce radied raletkl ft it po*ibla dial the ~10 (~ acts a a free radical scavenger to interrupt these reactiona, Tile I~lt,dy is being continued, (MS) c 3363 ,~_f~e_~p_nf_ ~c,~,!,~pn_~C!g?r~e~,~ P_~o~P~Sl.t~t~e~~ R1,102 Sample Series Nine nafr-cwnparfson teats were conducted and reneated an this sample series, The results have not hcen felly analysed, but differences appeared to be slight, with the lower c,p,i, more desirahre, Rt 216 Samole Series A sinple plir-comPatiJon lest usa conducted by IL new psckafle- basis panel between duraurier samples at It cpt and 55 cpi, lover cpi aanrlervcte nreferred (90X level of confidence), Results from a hrkce Research test on the same Jim~nlcl are not yet available, 0-90 Sample Series Fva FRlr·cmnr~arl~on nmckin~ teats were ermllclcd between It cpi,md 3~7? rCl,and 55 coi and 3,II rCl,~t II and St ms, lenl(Lhfi~ Teslinr was conducted on both the sLnL·ciaRrette. panel and the oackl~c-bnfs panel, In hath tests on each panel s!stn, then u~s nn omall prefennee (1~1. Icvci of ennHAmcl) for t~e It cpi;md 3l1a PI:i kmnfles, (CACI Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 __ _ ·30 · e 3314 Ef hcLs of Clrc~!nf~l~e_p~tlo~~Rok!?P, ?W~ pi 193 Samnle Series Three Fair·eonFartson tests uere conducted across circuoferenccs pt fixed Rkht on the ~in~le-dllareete panel, panel members expressed a strony( preference lot the low circlnnlerence sample (24 ms,) on the basis of better tsste ~u~l~ly and a Im3er level of irritation, three psir-comparison t1.91S were conducted across ~~rctlmIetence at fixed pressure dtoo on the n~nllle·e~9arettr panel, Panel members found fever differences under these circumstances, kit felt that the 24 nnn, sample VAR more ~IIltA(lnR. Tile rtafcrtfd sample appeared to be the 21 mm, asple, (CAC) e )521 Pvrolvsls of Polvethvlene Olvcoia An interim report has been sucmitted, The gaaeous pvrolvsate products of atbova# 200 that could not be condensed In cold traps (·1101C) were collected in I tubber balloon and 2 mi samples of this mixture were examined in the P ~ II a10 gas Ehromntognph, Two stalPless steal columns (6' x 1/4" 0,0,) picked with polymer beads · porapak "9'' and ParapR~ ''T" uere used, The follodng components were identified: HrtLoc, ethane, athylene, acetylane, propylcne, propane, t·butcnc, huradlene and aeebldehyde, Bealdea eorbowax 200, csrbowox j~, 100 and 600 vere pyrolyzed and the distillate in each case w~l examined in the Ietogaph gas chronatogrJph ulling a 10' x 1/4" O,n. stainless steel colutrm p~ehd with b~ 9~·1 on Teflon b It I~O'C, nut of seven peaks, four were identified as those of erhylene Glycol, dit;hylene glycol, triethylene glycol and treraelhylear glycol, Tbr condenaste collected In dt cold trap (·I]O'e) was examined In the same coluo~a~ st 45'0, The four components urre identified as acetaldehyde, proplonaldehyde, fi·bu~yraldlhvd~ Ind acrolrln, (jhA) 0 j522 Ivrolusls of HLdtoxybentoieAeld tltcrll_(~unffib ts tic_hfelntl Phenol is the ~win obfcfrlonable component in the pyolylis products of both Nfpagfn n·Sadlum and Nipasol H·Sodium, NipaRin n-Sodiua (500 ng) on pyrolpL gives 108 me of phenol, the yield being 433, (JPA) 0 1523 Prrollalssl WcRthct·rlcek~Contro1 ComPounda Preliminary pyolytic Studies of diphnyladal, N,H/-diphcnyl· p·phcnylenedfamlne and phenothLrine indicate the presence of i&thaw, ethylene, e~tanl, lcetylene and propylene · all In traces, The starting lrmteriala moatly distill out, Pytolyref of benzene and aniline also give identical products in traces, Oheno~Marine in addition gives hydrogen sulfide, (JIU) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·31· E ]111 Dile~tbl or CI b~ h~lLa ·d Y~e~ur nula of PI IsllIreh Lbor~to~ Ielort bl II LI teen iIIlloli, (MS) e 3811 Cillr hspt~tn ~alJ In Coljs~tioa with I~~bt.Q~~~ 89, 71~ a·204 g,,~,l Nicotine Llllfl(~ on two Iqlbl of cillr ffllcr ~~nnrld (I~r-PcrB bJ ~ilh~ Ipa two IInplsI fInnonted bt Ttl. Col It Blfs:tt II utll aafcnspttd eoltrol dl·ca ~irePlllg no dl~Iarane, bs· ~lcn Iny of the five Iapiee, nle dnEl bu bean ionarled to cbe lCIf ItlllltiOP ad f~lilOP 610Upl (Is) Ten pll~'e~llitQ tCltl rta eonductad b! the ainr(le-eiRIr Jlokin! yaels, 9Iliple e~l~n eoPli~tl~ O( two L·ls of lit-Wn I~a·t~tioa, two ttpel of I,T.eo. fermEntlllion ad as p0P- tlaatld. R~~ults iodicsten I definite aupstior~t7 of hir-tea a~DlsJ over non-frnnrad I~pl~~, vhieh in torn Ire aunltior to I,~,Co.'~ farmentItirma, The complete A~t-ren Icncnfntloa aroclll wan the heat md the tl·dr~ld t,T.Co, tfnanlatlnn the Yor~t. (G~) C rll DlrcloRlllt of lIrIaoeeium rlchnieu~l Plrlwleiun e~ud~lm hi bssn ulld to dlrctnins 1) thI Iffic!snev of I filtsr in the rlduc~ion of ImokI ~~ieity, 2) ths dllfetencl In toxieit~ bstvctn the uholl Inoks Ind the vIpour pbra of lak~, j) the difiersncl in toxicity bIeussn Ievllat correill bnnda of cfyrltt~~, tq~r~lon of imrnhilitILion tlrl for P~ iltIet llIverl Illtcr Ylnll, Ind 24 IlIyerl llleer KlngI without filterI, Ihovsd t~~l the fllterllll fern wII p Ire effIctlve In producing cflin~lJti~. u~ina I Q~ridte filter to remove pIrtioulIta ~tllt, imobllizslba tiwI for the whole Iokl Ild ~po~t phll of Icvel co~rc~ll brl*~ were obtlinsd, Although the vIloor p~ll ~1 1III toxic in ~11 EJFIII dlffereneeI in toxieitv between the vapour ltLlIe and whole Inke Pflld W(I 1 vorv Yidl nng,, 'lhs cili~ltl~il of whole Iloks end of *yoPr phIIe I~ lellnllcd I·r '1 comurciIt bfIP(II I(iPt 11I111I O'f II I ~~n~rd, IllllrI, 1lZ give dltiltiOPII fTI the I~ldlrd, of 1~ for whole In#ke end r~ for ayort PhIIe, An IttenPt 1111111101 to oorrel(lle the IIICICI weight of lIrlnicotine plr Puff rill i~billptioa ~lrl hot cigrrttcl with IllilIr tar Ind nicotine coatentl lhowed csnIiderIbll dcvinll6n ill their toxicitiel. ClibPDF - v~~fastio.soni -31- Because the method fnilld to shou sPv eislli[icnnt diffctenecr; Ln tlltl toxicil;, of veriou conlperciR1 filters ~od non·Iiltlr bnndl, it bl!; been decided to m3dify the apparatus in Itrenptlnq to ~nlke Lnxncbillza· tfoo time n mon aee~t~lte repnslntff tlon of toxicity, (~UI) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 __ __ ·33· E!lm e 4CZll2 The IffeFtof_Slle T~pr on the gfieica~ec~_c~l~!u~l~ hertsll ~iltarl for ler, Hicotine end lhenole ~~ eo~lt~lon of reeulte from peper lipped cigltettel rdr vt~l flus-eured end burlcy Lalf Irlde tohceo~ indirnted Ih~t the t~r, phenoll end nicotine in empke were filtered to the Inme extent, eellulole Icc~tr tlpe, otteohed to EIPllt~ll Mde with single Irede burley end flul·e~ld tob~eco~ from four etllk poelrlonn, filtered phenole end tet to the eome extent but they ehowed e reduced I~l~ctivily for the nicotine of flue-eutld emoke rrrelluc to thnt in burley emoke, in eddition, eelectivlty of nlectlnl with telpaet to ter wee obeervcd with the I~kl from lurlav lug end rutter ~ndcs but not for the leef end tip Indll. Ho lueh selcetlvir! wee evident for fluc·euted loka, nor from the one fermented tobeceo ~~npll tented eubeequently, Crey l~barllorf, in report no, L1OJ·R, confirmed, in the rln, our relultl for the burlly·teltlt~ eombinetion, However, In obtervlt~on,(ln RD·26j·l,)l~r the filtretfon of phmpln from ,burleyewke by aceeate filtars wee conl~dar~bly lower d~n thet fron tlul·eurcd l~kq wee not found to be the eele in out txperilne~, ~ I~11 Intlr·ljbot~tory Itudy, (vith 1,T, Co, lupplyifq the cigatlttrt end fittetl) produeed reeulte In good egreement, wllioh dilp~n~ad with the pol~ihltity of methodology difflran~r~ hut, inltmd, ~u(Bcltrd ~n intrineio difference between burley from different orillne, lmoke pi rllorllcntl, by erel, ebowed the CllnediJn burlrt 16 Irre e lower vltul ~~ln Crey'e butlly toblffo, Ihe dlvarllnel In rl~~r~ between the two I~botltorlll wee ettrihuted to thin differ~nec, Icr by thie time, deerelllng phlnolr filtretion by jeltlll with Incrwr~~ amoke ph hd been Ilflrtlin~d by I, Horeewell, O~S) C 41113 ~lnlny~rrrPl~os st ~~lil~r .lddit.i_ve An etcoholic eolltion of 1Dlr~~llrlOlldODI 11I Spll~d on 15 1, CI 3121/8,000 ~~1~~~~ cont~~ing d 1122 of pl~~tlcttar (tri~eetin), Ax lleohol eifltel lor ehtlnkins of the eA tilterl, the CoPtrO\ filtarlwere ~lleld~ith equI1 ~1II of Ileoho1, Itl~tla ~nd eontrol filterl were etteclled to the eigelette rode end ~1161 in filtrltion Iliiciencies were eonpered, Obelrved efffciency chenGee md levele of ehloroforn trtt~cebl~ Pieotlle ere given in d~e following t~bl~t ClibPDF - v~~fastio.soni ·3k· 2 Iflicimet Q·t~~ ~ol~i~~l· I ebci3 Pyrrolidone P~e, Itlrl IPbonols Frol~lP FM lIICN ) f~et~crlbl, bldinn '2 I ) ) I I I ) Ilcotinl 3 I · I · I · I · I · I · 1 7,6 I I· I· I la,l~t,a Cisla 122,3 913 lo (· (· I 11,1 C1S,d 1212 ~e ~ddieloP of polyvinylpyrrolidonc to tbs a ~ittlrl lah~acll the filtrlelon ~~I~eicncy for phcaol~ ~nd HCN, tsdueco the liltmelnn sfficlenct for TM ~nd ~erol~in ~nd Ilnl~ tbo t~r ~pa nicotlPe afIfeianfy ~chlogad, it is alJo ptoblbll Ih~e POI treated flltera uould prwa ef:ic~eioul in Iltlring th ~trlnl~l of olgnrotto Jnokc, bllcd on the rel~tian~hlp bctwlcn oildrcttc ItrenCth ~d ax~r~eelhle nifotinl Ilvlll clt~blllh~d by Crly ~a Raporr No, 1D 437·R, P~d~cr ntt is pllnd, (IJ) ClibPDF - v~~fastio.soni ·35- i: 5000 0 5111 _Prelimlutv rotllation~ of a ~pvm~rtn Subllit~tz The fonaulation of a cou~a~rin eubatitute with the Ingredienta cLllifiad under the vanilla note is being evaluated at the level of coumarin application on pipe tobacco, (~1I1N) C 5221 Hint Plavoura Two mint tkvoun (A and 8) were applied to cigarette tobacco (Pkyar'e nlld) It the level of 0,55, filter clgarattea rmnu· fs~tured from tbolL aamP[ea Ve, II control rmple ftvoutrd with menthol at the aame level, have been evaluated by the subJcctlve evaluation group, The ralulta indicate that aaPple A L superior to both samples (B and Control) concerning the overall cLractarLlio, (I~glN) e 5222 Influen~u[ ILvaurs an Rats end Strronth Lctovarv Iron ehotaJRl_ formerly filter CiRarettaa c 5324 Several flavour eompositlona were applied to Olayer'a nild Flenr· ette tobacco, Clgsrettea were manufactured from theae fLwured tecipea with "Ptune" charcoal flttea, A fLvoutad aample coded (BC +H) ahowed A n~Mric~ble improvement In tnlte and ~tnngth of Lhe maLL, 2uttbLmore, the patllcul~~ clteoll ulte vaa auceelafulty meaked, Two other aanplaa coded (BC +'e) md (PS P) vcre found at Inrcr~~t for (ke iqmvHNnt achieved in taste, in all rhev aamplea the ftsvour level of application ranles from 1 · 50 ppm on tobacco, fheae results were obtained from an expert panel, L larger panel teat is planned on wchinl·aulde ~0411~, (hfeU) C 5223 Lnehrl Boraee aa a Source of t~nthol The cigarcttls were made from tobacco sprayed with chloroform aolution of wnchy~ borate, Menthpl borers vaa prepared ~eeotd· Ing to tbs procedure deecribed in J, Qea, Sec. Japan, September, 1956; 37·2 (1953) by N, Hlno and S, hgi, After two days of Itonge the cigarettea were mokcd to a 23 em, burt length and tha menthol mainatream deltvert was dltetmifild( Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·3(1· eguRlln~ Bnthol on Bnthol &In~trrnn Tdbeeeo in T ~I Dcl ,tS ,Ir ,30 ,It The analyseJ for nmthol In M~olRean elwke vere repeated after three uetb hut 0019 tncu of ~enthol were found, The mese probable expl;mation for ehL Ihcnawnon is chic the menthll bone, dpcompond ~l~nll into ~n~hol and botic eeid, end rmnthol caclped Into the dr, Henthgl borlte is not stable in solution, 90~ I~okatt in the ~bot~totg IIPOi(ld n#nthyl bdratc·tn~ttd cigarettes and found the menthol aenection comparable to the Caneo manthot~~ed eigsreltes, (MS) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 _I ·31· CrXWO Product Dlsjffn C 9121 &tpctLeotalClaarltec KCelDCJ . PlolcCt SILrlm (R1·2!4 SerL~ NientLe and rcdlrkg sugar analyses on a recipe produ(:ed Tro~'964 end 19(15 crop tobacco (M·Z1~A) ed another enellatinp from the same grade distribution but vfrh the lug grades deleted (RI·214 B or C) did not: achieve! a chemical aqaal~icn that resembled tile Export "A" recipe any more closely tllan tho alrrcnr Player's Filter recipe does, n~e sugar levels of the present experimental series and the Player's rllear control vere founll r~ be approximately 2·3X higher than noted at this time k the Export: "A" tobacco, On the basis of the present data it woalll appear that the moderately hfgll nicotine and lov sugar catcuts o[ the Bxo0tr "A" recipe are being achieved by Ilainl dispr?pnrt!·natr 1U"oti;leJ of Ilp pldec. (BJS) C 9150 Otoiect Prune Slxt? triple filters, comprising one control and one sample uete hnd nnde tot the Swke Raaaarcb Srcup, (BRS) C 9182 variation in 4ntkolalta under Di~feren~ eonditions The Ipll~bt~tion rate of mentllolation from foil vol studied on cigarettes manufactured in tvo different types of packages: slide and aball, ad flip top, Two cartons of each type wore Idt at diffsrent bmpatleun and humidity conditiona, Every two to three days one package per location vr e~ovsd from one carton and the di~~rPutioluoi menthol in the psckage snd ~ln· 1Cclr delivsry wcre determined, After the constant delivery of menthol in smoke wit reached, the second carton use opened and menthol distribution and mannstrsa3 delive~w~a asaesaed on evo packages per each location, TABtE I eondieiona Iacation Temperature Relative Ihlmid~~y A -6 .. B 36 80 ·80 0 70 60 D 85 61 E 100 69 Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ~ ___ _ ·38· Slide ed Shell Pw~~~~ ~tlr 10 dill, p~~lel Itond st 70, 81 Snd 100'F laet rll unchal ftp~ the fell, but erntbol oninltrlm dolivory terehed o fonlltlnt Ilvll only Iftet 16 dnyl~ ~I PIEtllll Ita[l~ rt .b Ila 36lt, reteinrd uatlol or foil ~l~cr 11 11)1 of etorege, o1tbou8h r~lbl reInetteen Illl*tf ~e 36'1, tseehld e Ievll of ,16 ~lctlt~ ofter 16 deyS rrd in- fteeled In e fcv doyl to ,91 le6le18t, wn ii Lothol Ltlll of 1116( nod 1I11 I~cLlcl Cftol fO deyS of Irorlle fl4ctll~~l 6 LI11 Oellopheno 'F e .b 611 eoo 0 1,9 11,1 ,37 36 30,6 1,9 1~5 61,1 io 37,0 J 6,4 19,9 ~63 85 33,4 1 111 12 ,13 100 36,9 7,5 1,6 60,3 li6 rllp TOD lscksrrel Mllr ~ dsys leekegee elored st 7~ 1~ end 100'~ lolt I11 Mtlthol ftelP foil rd ~nrhol PLPltllll dl1i1l0 rlldlld e~Plt~t d~livlr~ tor ~11 loc~tian~, 68eio, the ~n~bol mlin· Itr~r dolltctl ot 36'1, woo tbo htlbllr, WU f2l l4ntbol LII11 of ~llp foP ~~ehll~ Ytlr 20 ~I of It~ll Tl~p~r~~url rop 6 ·b 819 113 2518 111 ,71 36 29,5 1,3 3,t ,88 '70 33,9 1,7 3,6 ,2 165 81 30,6 13 4,3 2 158 100 29,1 6,2 4,6 9,9 ,37 II la tot~l t#nthol nor Prekrlr It ·6't~ loeleiPI lr Ire to e POI.·jfOll Ifl~Dlt~OD of IltCO1 on eil~r~tel~, PI tt~adIlt of rlllol ee IPcb r tr tonpetrture hot ~~ 1011 to the cid~rstte id noStly bt pbllicel Eae~ctl k oP11 f!ro Fifalltcl nor Psx4cl rtl Illoctla IIP1·l1 for tootlol(l 1 low tot~l albol ptr nreknCe ul rslrlt, ClibPDF - v~~fastio.soni -39 · tool and Cdmeo production B_u4 Slide and Shell hcluaLa, 20 cigts, in (:ameo and 26 clgtsl in Koolp#kages, Both aamplaa were studied at 70'r, and 85"FI storage tempsnrutw, Aconstant menthol mainstream delivery was recorded after 5 days for Xool cigarettes from both locations and for Cmeo cigarettes 6 days on the cigarettes sloted at 70'1, and after 8 days at 85'r, laboratory Report No, 52·t has bun prepared, (~SIERP) C 9221 Evaluation of Dunllilr Tflbae~co_(l\ssoei:~een Isidl j'~jCCE$ ~fnll j:llrl Coal) mt reduefna sugar content of tie tobacco froln 95 nn, Bunhlll eigarerers ~J~s reported erroneously RI 17,72 in the pre~cJing puartcrry repo~e, Difficulties were being encountered with blr! reducing sul;at procedure at the time pllen this tobacco was hr!n;l evaluated and as a result dry were reported with some rcJetnt~n, 1 After rel3edping the situation the Ounhi!.l tobacco was resnaly~ed which resulted in a more reeson;lble sugar level nf 20,61,, (X.rr) 0 9221 Evaluation of Rt·105 Glr~etiPme~l Tobacco necipes 1Pt~ieee Seor~ Expcrlenta! recipes produced by diluting the teClllar du Kallr!er tluc·curcd recipe with 8X and 167; uncilrrd T,B,S, (1962 crop) ~~cre analysed for nicotine and reducing su4ar, Ihf saga data reflceer~ll a linear dilution of the du E~ur~et recipe with a 11 reduction In sugsr level bain~ achieved far each 81 increment of T,R,S, BI small aneieipaeed increase in tie nicotine content (,OS"h) clrrwpael!· ing bl the substitution of 82'2,1,6, could not be detected at the lowest level, (IIJs) _Sske Analyses · Ri 205 Slrlcs The purpose of this project was to investigate 1) the feeaihili.tv of producing a 1001ol filter tipped cigarette with tar and nicotine yields on a pet puff basis not greater than a du Mautiet Ring Site clgarettel 21 to explore the possibility of the Incb~on of rBS with s flue·curad bLnd, from an economic standpoint collpled with ILt of customer acceptance, it was to determine (1) that IIR(I~I! analyses were prrfot~ed on three suhsnmples of the IU 205 Series (hojcc: Score), One sample of 104 du Fkuriet strip comprised the control, nlrl other two Mmpln coPtained 8 and 1~ uncased TBS, Results of the analyses were In good agreement with calculated values, all three samples showing vary similar tar and nicotine yields, The ouslification, regarding yields on a Pr puff basis, was ~d~guPrel~ met by all samples, A memorandum has been iasued, (US) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·40 . Subheeive Evaluationa RL 205 Sample Series Three paft·eompsrLon tests were conducted by the linglr·clentcete smoking panel between these I~P1CIII The results indicated I dis' like for the 16'2 level, but were inconcluaive between the 0 ~~d Bt levels, Si, 211 Sanslle Series Three pa~t·eomparLon tests were conducted by the ain~le·cigarstte panel brtpctn Ive rcelpes and ewo Icall ct Tuc~e~L lurleg Stripe, Very few significant results are obtained, but preferences were towardn the use of T,8,S, at 8'2 on the Tilter Player's recipe, C 9223 kluaeion of RI·21l ExPcr~scnfal Tobacco Ibe~pe! (Rolcct score) Samples of the latest series (R1·211) of recipes involved in PtajecC Scote acre submitted tar nicotine and reducing sugar evaluntlon, In this instance the regular du Wsurier ad Player's 'Hild Strip recipes were diluted with 82 T,R,S, (8urley strips · 1962 crop), ~~in, as with the previous series, the utfliaatlon of 81 T,B,S, orodueeJ a rcllLtic reduction in the sugar content cl,n, and m'lnaigni[ican~ change in the n~eortne level, (DJS) Prolece Score · Tar and Nicotine k~llpla~llL 105 and 211) Two 100 ms, experimental filter efgacttw, comprising two samples of a regular Virginia bland diluted with both 81 rad 161 T,8,9,, were designed so that the smoke yields would not exceed those of a du ~hurier I(ins 31ze but would approach those of the 95 mn, Illlahill ctgstetta, The first qualifkation was met but not the second, in either yields or puff numbers, Subaaquantlp, the Bt·211 series repeated the supple with 81 T,B,S, addition but replaced the 20 r, tip with a 15 lan, tip of the same tow but lover prsssure drop, This increased the tobacco length burnt and was inatrumentel in hllpinr, to raise the tar yields and puff number of the cigarette, but not the nicotine contaat, A stronger Virginia recipe containing RX T,B,S, vis tested at the same time, (RRS) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 _I _ __ ~C~C ·It · 0 9231 Evaluation of St, roritz elnu,lrad se, srie~ meneholated oigarettea (OI sea, length, 25 Fig~~~lpkRI) have been examined for their level of laentholetion by the CIC ~thod, For menthol mainstream smoke delivery, St, kiez cigarettes were smoked to a 26 Iw, butt length, a h tlenthol on TOblCFo %Iciar~ 0,94 0,95 ~nthot on Filter rip nWlcigt, 0,43 O,IR horhol on Cigarette 1,37 1l43 bnthol on Packaging heltfal (mg) 2,47 2,09 Total Hanthol par packege (~) 36,12 37,64 Ehinstream Smoke mplfiee, 0,37 0.39 Egllivaltne loading (melgm, of tobeeeo) 1142 1,46 Tobreo Waigh~ mslcigt, loYI 1034 !li5) ne chtom~t~grlphle analysis of the 9e, Horitt figaretto extract revealed the uisrcnes of two other oonstituenta (peaks) in addition to menthol, it YU found, in 1 eompuison run against: a natural peppermint oil, that the two unlmmn peeks coincided with two peeks of the peppermint olt, This suggested the poeeibility that aoPT peppermint oil conetituenta could heva been used in the flevouring of St, ~elf cigarettes, To datarmine whether the two peeke were flavaur ingradianta, ttug ware individually eapareted in several chronatogrephie runs, with the esaistanca of a Itra~p splitter, ea identifioetion lln41plil indicated that the tao eanldtuantl were not peppermint oil flavour ingredienta, (eRB) Tar and nicotine yialdl and filter ~Hiclae~~l on the 95nm mentholated cigarette introduced by lot~nnl of Qn~da have bean evaluated, The St, Hori~r cigarette reeemhlea Dunhlll with tupect to Isnqth, p,),, average weight, recipe and filter tip, hut differs in pufI nlanber ad cigarette paper type, (gR8) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 ·42· O I·I .·~,Be~i~i~i~Y'~ 'l Prpiret Unssv ;i!!#s ll:t·216 S_erIes) L uas to be expected very similar levels aE nir.otlne anil rFlla:(a:: su~nr I·rcre Il'uaerucd in the Clo cxperimontJ1 tcci~l!s (d~l Imutl.rr blend) vh!cll differed only In tl;e cut~/fsch sueP.ffleatbn, TI·l! nicotine level or' ehasa solplcl ~aJ nll~~c!y I~et tilts 660 b·en o~taL?J in recent mrm~b for ehe do t:auricr recipcl (HJS) A proposal to extend the present '14nm Emblnl? filter cignrrttP to King Sice proportions, led to I design using the present recipe, st 45 c.p,l,, and a tilter of ncdim efficiency, The purpoae ~s to PtCh )ark Ten King Site as closely II possible on a total yiels and yield per puff belie, Vnfortunstely, tbs observed results of the experimental cigarette did not agree vith our calculated telull:·. smoke and leaf analyses on the control sample indicated st the recipe 9~ not giving the values incorporated in the design Ealr~l:l· toons, (BIIS) e 9251 Pralrcr plavera Hiid · experimental ,ia3rettes Using knovledge of the changes expected in smoke tar and nicotine yields, aln altering f,p,i,,eireumfcrcnrc, and pCI content, a series of calculations were made to design a cigarette chat uould be a cloae IPltEh for Poter Plain when uaing players Hild recipe, h good match could be attained for burn rate, tar and nicotine yleld~ Iborh on a par putf and total smoke B~L) and puff number but the ptellure drop of t8r ciyntll Muld be higha by ,P" (K11Sj 4 9261 Protect dv Hnurlrr · ht and Nicotine Analwel on eenuar and Ainn Site exPerimen~alC~nate~tel (C·90) The purpose of this study uas to compare the smoke yields from tobacco cue st 55 c,p,i, and containing tbs normal levels of PCI snd flgg, to those from tobscco cut It ~ c,p,i,, contslning only 50~ of the normal PCZ revel, The results of the analyses confirmed the expected changes in parameters, i,e, reduced pressure drop, increased puff number and tar and nicotine yielas, Th laagnitude of the changes In smoke yield (through the filter) we 8~ for thc'tlmo~nd 4~ for the $nnn cigarette, (IIPS) Clit; PDF -!::!!::!!::!.f3 StlC.i: 0111 -b3 · b~:~L W~TLltC i~dp(h · e.~g~ylet~lt ~,1 ildlaq8 one o.F du ~~ IIEplll ~lOdleld ~~~I ehe rb~cal rel level vol ~xmd to r.ontrio Ik~lric~nrl7 ~u~r IL7,t~e ~a~ nnhr ~~, suenr I~clJ ~hnn cht ontrot sonnple ((,I, recipe) U~s woe ~~rl· botld ~n aJplfql error ot ~1~ jlctorl letst in ~ta IE tile bKe th~r tile IptfCLPLe!d ·~diEfeltiOP in e4d'ltim (iPcrlall in tC: n~cottle end rcduoinp, auglr cmtanta of 0,332 md 0,4= rcnpa~c~~l!' ~as Icar d~n ~u ~nllgtlenl error Iwolva~ ~1 rld~ar dcto~llntb~, ~ain~lha do Eburier r~e~pl u~a~ ill Cds c~parbEntal ~cric3 o~- t~tlcd Ics~ nlc3tine ~ul noger ~~~ ~~~ hoon dcecreal In rsr~~ Im~p ba dd~ brmll, (US) G 3Y1 P8llct I~a fniti~l crllll err being eotfiod out, No conclulive rellulte rrh nvPllobll up to dltl, (AfflW) e lh]i rrallct n~~~ ~C~s!ngr~Lh~t~!yu~t~!,~~A~lorl~~ ~ r~it-eaP~ril· telts were oondoctod bt the inlornnl nine nnnol hctulcn unll~ of Old Port nine toh~eeo with two dilfsront fl~vour ~olvants, No diffarenccs were Ionnd in either lese, G 9~1 ho~i~el~pee A new brlnd hue been developed, Lrptrlnnnl ~llpl~~ will he ~nufjetutad in Crlnby, (UI1N) C dP ~r~aP Jib 81 ~1!~1 eil~r~ b~l~l Diplat eigere were received for IrlelRcat oE the length, prl~~utl drop, ~ull, lorol ollulold end nicotine content of the blolrl, filta ~d ~l~p~ indlprndentiyl For thl chlnicli ~l~yn the eilere in eent to the robaceo Irlrtlol SeetioP, I r·nn~ll hee been lllued, (ppg) ClibPDF - v~~fastio.soni ;,·~·~ T~:I~C~-3P I r I·nmr~·· Ill···~·Yn~,ir~r f ~~ E1 -i. c:e :: 1'· 6: ;;Ci ii Itj~ ~ d15 i A~~~J1: ~k r·" ~eP c; "Lr"~: ~ k~~,·· ,.,,sr , ·` ·1 , r 1.· ~3 ~·1 ~,· II "" !''"s:: ;r ~r* ~nf ,, ·u L i4 p it '~" 'P·ii~F~. ~; ·.:~7 N O W N O ClibPDF - v~~fastio.soni