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131FLONVIV 4Y. '3r<>13A.ACCC>
1600 Nhn R21 Street LauixviUe, Kenhxky 40201
August 12~ 1970
REECE
Dr. D. G. I. Falcon
",asa
arch & Development EstablLshment
Brici;h-Anierican Tobacco Company, Ltd.
Regent's Park Road
Millbrook
Southampton, ENGLAINM
Dear Geoff:
Having reviewed the proposed design for long term
janus experiment,
B10, we would like to pass on our observations and
comments for
consideration.
In the aronosed design, the additivity of yeast
treatment of lamina
and reconstitution are invescigared by both ?_TS
paper and PCL processes.
The Schweitzer process combined with yeast
fermentation of the lamina
could well be sufficient for establishing the
extent ofaddizzivity. This
requires risking that results on addivitity are
directionally the same
in boch Schweitzer and PCL processes. With the
long term interest
apparently shifting to paper rather than PCL, this
risk does not seem
unwarrented if more worthwhile experiments are
being delayed.
The following three samples are suggested for
consideration as
replacements for the two PCL samples, 3 and 5, in
the proposed BIO design
given in S. R. Evelyn's document of 8 July 1970.
i. Yeast Treated Flue-Cured Lamina With 12% Added
Sugars
Yeast treatment of flue-cured lamina gives a lower
test result than
untreated flue-cured lamina. The overwhelming
quantitative chemical
change in fermenting flue-cured lamina is loss of
sugars. Sugars are
then suspect of contributing to higher test
results. Unless Lnformacion
to the contrary Ls developed, sugars must be
assumed extremely imporcant
tobacco additives useful in concrolling burn rate,
handling properties
and subjective smoking response. From both
theoretical and aDolication
views, che influence of sugars on test resul:s is
necessary information.
Since the proposed B10 design includes (1) TC
lamina/scem (60/40) and
(2) yeast-treatad FC lamina/stem, a (3)
yeast-created FC la=i.-ia with
added sugars/stem sample would be a simple and
timely means of gaining this 110
iaforr=tion. 'rhe sugars added to the tobacco
should be a r..-x:ure of 110
glucose (44%), fructose (40%), and sucrose (16%)
which is. according to our Lrl
estimate, the relative amounts of sugars in
untreated flue-cured tobacco. r\-)
C..11i
BATCo document for Province of British Columbia 2
November 1999


Dr. D. G. I. Felton
August 13, 1970
Page 2
The addition of 12% based on the weight of
fermented lamina seems an
The
appropriate level of sugar return.
11. Schweitzer Sheet From 60/40 FC/Stem. But Stem
Extract Discarded
Schweitzer sheet and Gerlach sheet have given
reduced test results
in German experiments. PCL has failed to yield as
significant a
reduction. The former two processes have in common
'no loss of stem
extractables. (Not strictly correct with
Schweitzer process since losses
do occur but are minimal and are roughly equal for
both lamina and stem
extractables. An identifiable difference between
the FCL process and
both Schweitzer and Gerlach process is, then,
discard of stem extractables.
A sample in the proposed B10 is flue-cured
lamina/stem (60/40) manufactured
into Schweitzer sheet. A sample of the same
tobacco (FC/stem) manufactured
into Schweitzer sheet with discard of the stem
extract liquors, w-I'll
provide information regarding the importance of
the return of the stem
extract.
!!I. Yeast Trea-",.qent of PCL Slurr~ Prior to
Casting
Yeast treatment of tobacco followed by processing
into reconstituted
sheet is not an attractive Droduction procedure.
Yeast fermentation of
tobacco slurries is rapid and could be a practical
process step in sheet
production. To be consistant with BIO design,
Schweitzer sheet process
would be more desirable for this sample but
approaching P. J. Schweitzer
to carry-out the fermentation is not acceptable.
Therefore, the PCL
-:,rocess wculd be used assuming the information
is applicable to the paper
process. The sample for B18 could be made by the
PCL process without a
PCL control since there are two control samples
already built into 310
for comparison with historical results. The yeast
treatmenc of the slurry
should be carried out after discard of stem
e~!tract and addition of ground
lamina.
Two series of samples, differing in the sixth
saimple, are suggested
f o rconsideration. The 6(a) sample is preferred
by us over 6(b).
(1) Flue-Cured Iamina/stem (60/40). Control (B4)
(2) Yeast treated lamina plus stem
(3) Yeast treated lamina plus 12% sugar/stem
(60/40)
(4) Sample (1) manufactured into Schweitzer sheet
(5) Sample (2) manufactured into S,;hweitzer sheet
(6)a Sample (1) manufactured into Schweitzer sheet
but stem extract
discarded
(6)b Sample (1) manufactured into FCL but slurry
yeasc-treated after
discard of stem extract and addition of ground
lamina.
We appreciate having the opportunity to review and
comment on the B10
experiment and hope our comments will be helpful
in establishing its final
design.
Sincerely, C=)
1. G. Es:erle
r\-)
~';G bf wl 3 4 3
cc: Dr. S. J. Green
Dr. 1. W. Hughes
BATCo document for Province of British Columbia 2
November 1999