148/13
3 M MEETING & 28th APRIL, 1969
The futi-e of lona-term skin vaintina tests at
Battelle
There are probably sufficient funds already
allocated to Project JANUS
to allow one more long-term experiment (Experiment
99) to be completed,
although it may be necessary to extend the
contract in time.
A new contract will be needed to enable further
experiments to be
carried out.
For the next few years, the long-term mouse test
is likely to remain a
major test by which modified cigarettes are
judged. Inhalation tests are
notyet developed, and it seems essential,
therefore, that the mouse test
should be available to B-A.T.
As specified facilities/personnel are required for
this type of work,
it would be difficult to switch from Battelle in
the near future and undertake
the work elsewhere. It is suggestedt therefore,
that a second "five-year"
contract is negotiated with 3attelle.
It would be possible to start four experiments in
1970 (Experiments
39 - 812.) It is suggested that these experiments
are allocated to the
examination of samples of cigarettes by the
"classical" procedure rather than
examining new forms of testing such as the use of
hairless mice or instant
condensate. The complete results from Experiments
30 - B12 would not be
available before the end of 1973. Consequentlyl we
should endeavourl from
Experiments 80 - 812, to produce a "package" of
results which would help us
to design a modified cigarette.
In an attempt to remove this statement from te
realm of a "slogan", the
factors involved in the design of a modified
cigarette have been reviewed to
draw attention to the gaps in knowledge.
Cioarette Darameters
c:::o
The effects of changes inxerious cigarette
parameters have been, or are C:~)
currently, being, examined and the results will be
available in December 1973. 1.0
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-3-
Sheet materials
This, it Is suggestedp is the area for major
effortg although it is
being partly met by Experiments B3 and B8.
From the comparisons at Harrogate of Folie 3 and
Blend 3 and Schweitzer
sheet and control blend together with the JANUS
experiments 33 and 34, there
is an indication that Drocess can be an Important
factor. Further information
should emerge from the second series of Lokstedt
tests. There would appear
to be a case, thereforel for further work on the
effect of theprocess used to
form the sheet.
Perhaps of equal importancep is to determine
whether the activity of
condensate from the "PCL" is dependent upon the
nature of the starting tobacco.
Additives
The eff-~,ct of adding nitrate to tobacco
undoubtedly leads to a reduction
in tumorigenicity and indicates that additives
provide a possible means of
producing a "break-through". It wc~uld seem well
worthwhile, therefore, to
have one experiment in the B9 - 312 series dexted
to a study of additives.
Clearlyp if chemical analysis of smoke can guide
the choice of additiveg then
it should be followed. It is suggestedg however,
that there is a case for
considering an examination of a series of
non-toxic additivesq which we know
could be added to cigarettest on an almost purely
ad hoc basis. Perhaps,
however, this could be amalgamated with a study of
the effects of smoke pH.
Filters
To datep filters have not been shown to reduce
tumorigenicity. Some of
the current results from Huntingdon, albeit at an
early stage of the experiments,
can be Interpreted to indicate that a high
efficiency filter might be beneficial.
Stating the obvious, we should attempt tolake
advantage of any results from
Huntingdon.
CD
C=)
C IA/S4 1/ 1. 1. 1. 3
21 st April, 1969
CX:)
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174/2
TESTS : LUNG MWURS
At the 16th r1C Mdeting, it was noted that, there
was some confuslon
over the extent to which lung tumours occur In the
control animalst
(.'.',inute 162. )
To clarify the pcsition, Battelle have sent the
following infor-
mation.
Unpainted Solvent-paInted,
Controls. Controls.
Num'-_*r of animals In group. 128 128
Number o
animals su-_,~ected to
114
25
1
histological examinat' ion.
Lung disordexs-
Carcinorma of the lung alveolar.
7
Bronchial carcinoma. I
Tumours of the alveola:- cells 4 10
of the lung.
..Ixed type malignant tuffours of
the alveolar an6 :3:onchial
epithelia.
Fibroma. Z - I
Pneumonia. 36 21
Small cell neoplasns in the lung. 1 2
L;ymphogranuloima of the lung. 1
Lymphoreticuloma of the pleura 1
pulmonalis.
%sothelioma o-- the Pleura
pulmonalis.
It is possible for an animaL to have more than one
of these
lung disorders. Battelle have given the numbers of
animals with lung
tumours as .
Unpainted controls 16 from 114 animals, i.e. 15.F,
Solvent-painted controls: 24 from 125 animals,
i.e. 19.25~
Combined controls 42 from 239 animals, i.e. 17.61,
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"."hese figures are slmiliaz to those obtain-:?d
by Roe at the
C"nester 3--atty Resjazch Institute. In a draft of
a forthcoming PU:311-
cation (TR.C document G 740), Roe reported that 54
of the 400 solvent-
painted mice had lung tumours, (i.e.13.55X).
(Please note that unt-,1
the paper I's publi5hed this inforretion is
confidential.)
In the Day publication on the .2irst -Main
Experiment at iiarroo-ate
0
Or. j. Cancer, 1967, 21, 56) adenoma -f-the lung
is recorded as t'.0
cause of death of 32.~,~ of the animals. in a
recent TRC document,
G 3615, hoviever, Harrogate have po -.ted out that
this is not tne tota-1
incidence si,-.ce lung adenomas were also found in
other mice for t-,111--ch
a cause of deathwas ascribed to other d1so.-6ers.
Indeed, wher, the
- 'de,
nc., -ice of adeno.-..a of the lung is extracted
from the original mouse
a ds of t- 'a, n experi-ment, the compaxison w-1
c
r .e figures quoted by
Roe -a extremely close.
I
CIA/ S011 1. 1. 1. 3
4 th July, 1969 C=:>
110
%-0
(.-n
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