GR&DC RESEARCH FROGRAMM-
PROPOSED REVISIONS FOP, 1984-1986
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Research Conference, Brazil - AqtLst, 1983
GR&DC Research Programric 85
PROPOSED REVISIONS FOP, 1984 1986
Worh Area 01 Biological
Cencrnl
The design of cigarettes of reduced biological activity will receive
additional impetus undar Project RIO. Initially, activity will centre
Oil Lhe deripn oC cigarettes based on the current "state-of-the-art".
11owevcr, as t1v_- inatrix of biological activity against product parameter
is not complete, refinement of designs will be dependent on the comple-
tion Of necessary basic studies. Over a longer period of time it
sbould be possible to capttalise on some OF the current biological
research projects (and related tests) in relation to the design of
cigarettes of low biological activity. The development of new test
methods will b,@t strictly limited to those considered essential to the
evolvement oil Projtct RIO.
Progrnmnie Rev".sfons
1. 1Yrod%1CL!-, OF rcd%iced biological activi.@y
A systeinattc ovk1luatiun of product parameters will be the main activity,
a s To nuct the longr-r-tcrm needs of Project RIO,
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collab-irativc %:urh wlLh the Combustion Group (Work Arca o5) will be
initiated.
2. Sidcstrann, Smoke
Studies into mutagenic activity will be given nore effort as part of a
large programma aimed at deterntning and controlling the biological
activity of sidestream smoke.
3. Short-term test related to cancer
In 1983 the demand for the first mutagenicity test. the Ames test,
excecded expectations and consequently the planned development of a
second test, mutation in ntaminalian cells, ties been delayed until 1984.
The short-term test based on the preferential induction of Cytochrome
P448 will be intensively studied, mainly under contract, since recent
work surZ;CsLs it may have utility in predicting the results of long
term skin painting tests.
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4a. other short-term tests
The method for measuring irritation. based on mucus secretion, is now
developed and will be evaluated for sensitivity to cigarette design
chanaes with a view to its use as a biolo-ical test.
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4b. Developnent of models for atherosclerosis
All work in this area will be carried out under contract and within the
year we hope to deter-nine whether or not the model will be useful in
lookinr, at Lhe effects of smoke. An extension of the study, using humans
-ind looking at acute and chronic effects of smoking on arterial function,
will be accelerated.
4c. Scruin glycoproteins
Clianres in serum glycoproteins in animals are being investigated since
it is known that such changes occur in hiiiiians, possibly at an early
stagc in the eevelopment of various discascq. During Lhe next year it
should be entablished whether this area of research should be continued
or abandoned.
4d. OLher tests
Some further Contract resenrch Is likely whon requirements for tests
for ndditivcs fell outside the range available uithin GR&DC. At present,
worl. on Eugencil is scheduled to be completed by IaLe 1983-
5. Smokinp and regulation of enLLU _t;-Ia!!cc
An external cortrnct has bcon placed to explore the role of nicotine in
modifying the dcgrce of wcight loss/gain by smokers/ex-smokars.
G.- Tests involvinn liumans
Tinc study with Professor OppS will f inish during 1983/4, althourb the
niih.-;tnntial anount of urine atinlyucs requircd mcnins thit final results
will noL I)c available until 1984/5. Furthcr work in this area is
unl ihely.
Collaborative work wiLlt Dr. 11tgenbotham, Papworth, involving lung
fkinction tasLs, will continue and will also involve studies into
variations of smoke inhalation pattern with change of product.
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