I
-KrIMM CAT. SUB-WJ;TTn OF T.M.S.C.
Ls you probably are awarep we at Southampton are
still. in
the early stages of development and eonseqmently
we feel that our
remats~~',~ ~& are very tentative, do not justify
lengthy
4 wosition.
Howrar, it is perhaps of - value if I Oats at the
outset our objectives 4which ve are using to guide
our t%4-1~4nC and
vork. We haTe chosen to concentrate initially an
the neutral
friction of amokit, since this may be hold to be
the fraction most
suspect from the Health vioupoint. Our vork is
directed tomods
a qualitative analysis of this fraction and
especially of the
polyeyelia hydrocarbons in It and coupled vith
this, we ax-e
conducting a quantitative analysis of the same
fraction for
Although such an analysis is an essential
praliminary
v9 fic, not content br-this alone. In order to
find an indication
of hav these compounds ari" in w2oke ve are
andftTauring to find
conditions iihich will bring about gross
qualitative or quantitative
changes in the woke somposMon. Acoarding4p ve wM
use the
techniques uhiah ve are at present developing to
as* whether thwe
is a striking difference betwen the stoke sition
arising from
the 4,n4%'A' or terminal halves of a cigarette or
betw~ ft*day
wwarod or aged oiguvtU Urs. 16 ax-9 oontamplAting
an actensics
of this work to different tobaccos and to
cigerwttes mode of blended
tobitows idth added casino and flavowdno.
For the quantitative analysis for bonspyrwe in ~r*
nsiz&
to a very great /the methods eval4ed b7 the
Research Department of
LT.Co., to uhan we are very inddbted for their
%holahearW
co-operation and holp, in this matter. This
method, as ym kwws
is b"W an fluweacence spectragraphy of cbr=&toVWM*
frsatices,
cc
0-\
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BATCo document for Province of British Columbia 3
November 1999


- 2 -
ihich thereby leads to a ooncentrate of benspp ,
which to then asmayed
by SomParizon with added and external standards of
pure benspyrane.
Dr. Bentley, I know, All not obj set to my saying
that we are
subjectiug the method to oaraftl acamination. to
check its soauracy,
reliability and efficiency. So far, we can say
that it is very
sensitive, the Ii-I of detection, of benspyratm
being around 0.025
anJ, when the due precoxUous agLinat atmospheric
ocintuLination of
solvents etc. are observed, it in very reliable.
The scheme of qualitative analysis we are using
has bow
devised by Dr. Hughes. Briefly, it consist# in the
preparation of
~ neutral friction by peroolatim of the total
mmoke condemmto in
~ suitable scavent through columns of Zeo-Carb 225
and Do-Aedits 77
suCcessiT91y, a procedure which is &W-active,
since it enables the
aoidic and basic fractions to be rooovered in
stages by "ad slution.
The neutral friction is fractimated by
abromatograpb7 an alumina,
and other adsarbents, the course of meparation
being followed by
ultra-violet absorption spectra imd by
paper-ahramatogrspMe tedmiques.
By thin means, the presence has bean dwis of
acensphthme, naphthalene,
PT- and of a long A,4- paraffinic hydrocastan and
a short emperinant,
bas shown a differs as betvism fresh tar and aged
tar.
Ma P77OUNU at different t4mqwatureg say =W M
*M-aiable eff-t an the GaMositim of the products
no famed, we
-Ting Out auvfa asaxwomants of the temperatures of
burning
Cigarettes. Ve uss thermeswVles, of platimal Lj%
Pt/Bb LU07 in
-r,J-+-i- with a Shart-patriod galvanometer with
pbotagrsphis resording.
Rovalts here too we also tentative and ve do not
wish to enlarge
van then at F"MA.
Throughout the wark we are using standardised
eigarettes
canditiowd to appa :Lmate17 12j% main santent and
selected by
both vaigh%, and dmv resistance.
would appMajoe that the Reg-ffixgh and Devalopagnt
lebarstary
of B-A.T. is still very zw* I-- i+,& infucy. In
viev of the tmUtIve
character of our results todate, whiah haw been
mostly directed t*wLrds cc
0-
proving the aVi*W of am techniques, I have bwe
brief. cc
DU
BATCo document for Province of British Columbia 3
November 1999