NRIVATf: M. 0",'"i-FIVETMAL
LGF/MV46D-2
Dr. H. Goinahr,
Battelle Inst1tut *.V.t
Frankfurt M Main, Z-13o
WleabodwMatrasse,
FMTRAL GE;r1j.*j 1"T_;U"J.jC.
Dear Dr. Gomahr,
11 th Mvember, IW
Thank you for your telephono call of yesterday and
for the
sories of letters which arrived duriM my *boonc*
last month.
while I have been *vay# a considerable amount of
cross-check results
on Sj7,plo U.183 have bw carried out, and these I
am reporting to
you below. To deal with the xmrous topics which
have arisen In
the past month# I will Itemise them soparatelye,
ci.;=qtte Welahts - surlev 59M.Aes
In your letter of Mat October, you referred to the
fact that
Samoa 2)7 and the followiml mabors muld have to be
sorted at
a weight of thirty-three cigarettes to the ounce,
as the yield
by the declared weight *I twnty-olght cigarettes
to the ounce to
W*-A*. I quite agm with you in this decision. At
the tim the
sa=pl-. cigaratUs ware sent to Southampton fna the
factory Vtwre
they were manufacturedt an accmpanylrsj latter
noted that the sanples
of :3urley rag had gz*stor filling capacity than
vas expected, and
that the cigarettes had been made, at a weight of
thirty-two cigarettes
to tho ounce at making wolettas,. Since a certain
amount of drying Is
catrrW out following making, the cigarettes at the
time of packing
will b2 rmn lighter andp tWeloreg your suggested
fIgura of thirty-
three clgsrott*s to the ounce Is 1wobobly correct.
Lianin Analyst$
I understand you were Inforned by taleitone by
I-Ire 3mith that
our result for 119nin for Umple W.83 %as now in
good awxszeint with
that found by you. The reason for the now value
found by us Is
/tontinued
-Cbb
\0
BATCo document for Province of British Columbia 3
November 1999


1 ~rw H, CWMAhr -2- 11th Movembert 1960
unexplained, but the agr"Ment 'Idth your result to
mw very sattsfactory.
As an Indication of this, I quote below a wits of
mas-chock results,
two of Wdch you vdU have already r*"Ivsd.
SmathemVton Frankr"rt
5 o02 5.05
moil$ 2*57 2*72
0,145 5.11 4*84
W. 237 2.74 2462
W.2" 3.40 3,42
W.2c 2,96 3.01
W.250 3*94 3,91
Srm~q
a
-lt1h the exception of t
&r, which I deal with below,
I record
for your interest t*tal "ties of cross-check
amlysis for mok*
constLtuafte %hich havu begn carried ovt.
228-0 So".A2121 southamriton Frsn'Jurt
(a) Aldehy&s Pg./cig. WAS 16001 1,12
Voila 1*36 l.a)
W.145 1*25 1.34
W.1W 1.31 1.36
(b) ltdcotina, sq./cig. W.111) 2.19 2. z
W4180 3,17 3.11
(c) roul acids M74/cLo. 40180 3013 2.93
(d) Vulatilo scids M./clg* 116180 1o74 1,72
Total baseg an./cIg. iielso 1011 1,12
(1) Volatilo bases mg./Civ. W.180 0v50 0149
It would seem Im thow rosults that our woke
analyses for all
Gonstituents other than tar appear to be In wry
good agrament, and
I s" no reason why swko ainalyses should not be
continued for *11
th036 constituents othor than tar. It would# of
course, b* useful
/Continuod
OIN
BATCo document for Province of British Columbia 3
November 1999


LT. H. cowl -3- Ilth Novesbar, M~
to 11ce1vt Pft4*d10 Indication of *me of tM Values
V61ch you GTO
findingo w that we may we*&-dock at x*nd=-,
CellU1010 A-MlYsts
the values oddch you quote, In your letter, dated
24th fttobor, are
certainly ~what variable for *we *nq4*&. Other
&Wles app"r
to be amh aor* cmistent in the values I*wW. . This
Indleatts to go
the rweessity for maklm at least three
deteminations of cellulose on
ovary unple. Where the On* values or* v4doly
scattered, It way be
Amessary to make a fourth or wore
Chlor0-INTle AtId
C4% reflecttoo, we ore rat haMy about the vothod
agreed, In which
the standW callbratlon curve Is made using
chlorogmic acid In an
extract of U1.145 tobacco. We f1M that yo can
determine, & calibration
CUM In the absence of Z-ample U.1450, In which the
line Is displaved
UPRU4 t* a constwit smount Wicating that Smple
1;.145 contains about
0.2s' chlorogon1c. acid, The use of a calibnU4n.
cumincorporatiN
10.245)for Durloy tobaccos containing less
c4lomgrdc a .cid than does
this samplay would nun wall nagative values =1ght
be reported, and
this Is clearly an absurd nuat,
Cur Dlo-ch*mlstry Group to also Intorested In
methods for the
analysis of tobacco for rutin wW chloralMnic acid,
and they haw
examIned Indepmviently the mothod agreed betvamn
you and us. They
find a calibration cum for chloralmde acid in VA
Ito-pro;*.-41
In tha ohs,-rsce of Sample A.149 which to
Identical to that fowW by
~k-,Ith. I list below the series of ctoss-Oacks
which hav* been
carzted out durl" the powt months-
a-%TTr-.I,T.z
~Fftft
1 :01j~rbU~2a
C4 tion. NINV flIft
Oarvo - From C" frov"11 turvt from r3f. vour
Chlom'70nic Chloronente Chloroqenlc 14ttaT 7th
Acid ',:xtract ftcld (1mly ~cld + -`xtrect
C-ctobar).
145 1,*
4'.88 2." 2.06 31008 3.17; 3* "M
5*24 5.10 5.23 5.36, 5.3&
6.18,0 3,23 3.16 2 7
OXS - 00247, 0,452 -
145 t5.22
A~nMnuod ...
CP\
BATCo document for Province of British Columbia 3
November 1999


rX. H. Gofthr 4 11th HDvewwro 19W
. we muld, theraforelp again like to suggest to u
that the
chloragonig acid calibration cux be soft in ow 0=
of Ubaoco'
so that the Burley vxqdos vd11 be found to =ntaln
a null awAft
of chlorogenIc ai:JW. go have tried the now mothod
you SuMsted
(Your Utter 7th October) ca Sample WAS e'nly, and
for this we foWW
3.36% chloso"r4c acid. The mothodo, bowwarl 41d
not seem to Wer
to us any sdvvnts~ps ~ the so% phla MVthW 1kt
Present Wing
used. I thU* you uJU opoe tMt Ak roasomble degree
of concordanco
Is found betw"n us# *spaclally for Sample Or* ve
vm%dd 1 Lke to
kmv %Nt value you (W for shlow9onla acid M Siople
W.180#
ft-bilo I was In CarAW40 I learned of a now **thod
dnvl*pW
there which pemits the estimation of rutIn and
chlorogonic acid
a1 multarwatisly. This 4wpia Is a chrmatoMphic
methods, In which
the spots are daUcted using a fresh solution of
forr1c chloride/
potasslura farricyan1da. The pope" are then washad
fro* of th* blv*
colour this reagent possews, and &ftor
dryingp6acut into strips,
rend*red translucent by means of elfteral all# vW
the strips are then
passed across the alit of a tnnmlsslom
donsitamter. The IntwMted
aroa bar&ath tto dtnalmetric curm In linearly
sslated to the amwnt of
chlorogenic acid, or rutin present In tM spot. tho
method Is
accurate v0 reproducibla,p but I am unwilling to
sunest at Wesent
vet ab&Mon our vjms*ful m&thod for ritin and the
present cethW for
chloroganic acid# just when agroemont swm &roww
th* cormr. In
future works, w mays, of courso, find It to be
advantageous In thet two
wstimatLans can be carried wt on a single extract.
Tar Analvsls
I still find It difficult to explain all the
festt:rae of the serles
of cros3-checks we carried out durin SoAmber. In
particular, I car=t
explain why you should. reproduce our value with
cigarettes saft froto
SDuthamptao.
!'urLn.; the last wnth, vol, too# hay* fo-&W
thats, d-2;*rvJ!"c
u-on the size of aliquot employied, there Is an
Lnervase of about VX4,
in c.1unging from ona cigarette to two, c1garett*
altqwts. we have
establiW*d by an Independent cross-check within
thesa laboratories
that zkrithle analytlcal tochnique &9rees exactly
with that of other
analyst* he-re. We hav*o onco wra, confiroed that
the" lit a c!ifferanca
In the values (*wW uslig so Ethel Ak. 6 3boking
Machine and a machina,
of our own design (W cano, In facto Obtain YMM tar
figure using a
B-A*T. &I-achine, but then the agreemat in woho
nicotine Is lost).
ON
BATCo document for Province of British Columbia 3
November 1999


Ith Ibvember, 1,)&,
rro 14* G"hr
I contIMM 'to belim, thoretbre, that we &hM4d
still us* an EtA*l
14.6 ftoking machine to compre with.you. the
followirra tabli, will
show you the exOnt o t cros*-checks. wo have &We
j-
4 b. Ci to M... .10. M&fg 911- HO. ~Vf f
(1) clectrostAtic rxVcIPI_
t*Uon
*x*L-q Mad4m 4-thel Uk*6 5 1802 17*3 M.5 16.2
10 21.6 170 M7 16.3
5 21.0 17.5
10 23.1 17*4
(11) C.-Mbridgg filters
Ethel Mk.6 5 2L7 .1607 IRM.2
lu .77.4
Not correct for blank value
L US W. MCS
d
M zlectrostatic Precipi-
tation
LAW Rk*6 5 19.0 17.4
L; W*2 17,91
5 2;o6 1709
10 22o7 1015
(LO.Cam.bri(Vo filters
I.,11hal. vk.6 5 21.25 18*4
* Us. ML !40. tuffs Other tab.
ILZM %I - C
z>vkad
(1) Electrostatic Prect PI-
Ethel 5 WAD 16.5
repeat 5 22*3 15.8
10 254 16.1 23.7 16.5
U.-AeTe 5 25*4 15.9
10 2)*5 25.6
Inde,,-qndent check
Ethel hk.6 5 23*3 16.9
D-A%To 5 25.4 1405
11.11) QW-btld-90 filtOrS to 23*7 10.8
Ethel Vk.6 5 V. 45 1509
BATCo document for Province of British Columbia 3
November 1999


Dro W Gamahr lith Nev""r, lgc,6
I f1m It diffloalt, to colkwo OAO "MUM With
Cambridge flitems
with thou fousid by you, Sim* you roport your
results vdtNrat . .
COMCtirog for the bl*a value an the Cambridge
filter# Cmld you
pleas* indicate what an Vw levals, of blwW,. per,
Ulter which you find*
and 1A futowl, Mrsoct 1pur values for this!
An SSVOCI" likbastay of *as MAch usas 19th9l Maft
6 wW
:~aA 7 Q-41no Vochints; hios ban experietwIrg
diffIculty L4 ObWAU*
ropm-duable, tar figuns. The *&uses of this have
bmm exu4ned
closely &M It would Som thA, elthau-3h each chwnol
c*.*t be adjusted
to take, a 35 al. Wt Wan. ao cigmtte Is lasorted,
thl a volum Is
redmod *An & elijarette Is placO 14 oari" with the
P"Kipitat"j,
own s1thaugh. tho flow mtor t1"t Is brouqK bm* to
its orl7tral
rasaing. Tho causos of this PWOM6402 a" at PVSQftt
obsc=*-
" are oxamWN this foatura aaselv9s an our em Ethel
Vark 6 ~Uhlne
usIN a rAw p4ttern c1garstts holder which emblts
us to reas-are the
VOID-A G~ the pull at either OW of tho clpr4rtt#o
I will se.-4 Y*U
details vkwn we have ampIttod.oa *xaminattoru
It scoas U m that tho bost salutiomyould be for
Pr. :Zrith and
vkte, visit you In the next low wooks W for 3r4th
to mrk alongslee
your vw-,t asslotaritiv. u-Diro the same viakin-2
machin*61, ap.-aratuat OWn.91,
otc. At this tin* too could. brLv with us tho
devicaF for vol=e
meatzrino] and "t whether tho uu Owmanm c=urs with
your mothims.
C-) torts
Eubject to your o3ro=cnt to us2 a calibration
cvrv-- br ch1*r*q*n1e
*c1d in tho obtance of tabacwt *M tQk a
satisfactory crass-ch2ck with
I think, we my coma.%* this analysis.
All %='k analyaos othor then tar &ra In good
agreemantp and ve ray$
th*raforev c==nce Va&t.
Tar awlysis sh-mld, howmr, a-alt the res%at of a
stdt-b~-slde
cross-Oacd% pozfermod by Wth at 7nnkfurt w1th rjr
assisunts.
; hope yali are in &greemant with ttms*
conel'usions aid that they
oaw yo= difficulties fairly considerOly. I wIll
valto ye,-.3 &01a in
tht roxt fe" clays latti*3 you know details of the
dot* of cur proposed
vWt " asking you to rosaw acvy-Wrtion for us*
Yaw inaxtirt
I-* Ge Felton
c.co Battelle, Londoj%
CJ1
BATCo document for Province of British Columbia 3
November 1999