O'G2 7?. C-21
P, .... .. P"- Research
27 th February# 1974
DGF/PSD/5-1-1
Mr. R.M. Gibb - Montreal
Mr. R.S. Wade - Montreal
Herr II.E. Sottorf - Hamburg
Dr. F. Seehofer - Hamburg
Mr. R.G. Nicholls - Sydney
Mr. A.J. Kruszynski - Copenhagen
Dear
J3 joast-ay of Reconstituted Tobacco Shoat3
I onclosep on a strictly confidential basis, a
report of
a Mouee-skin b3oassay experiment on condenantes
from a 801-103
or rocoaiitiLutod Lcbaccos wado fratu a coLwuon
tobaeco blelLd by
the following processes:-
(a) Schveitzer LIR (low tar).
(b) Tainag-Ilauni.
(c) Arenco DIC (equivalent to Gerlach).
lb:,ovaluation.of the results leaves a lot to be
desired;
for exa lo there I no age- standardii3a tion
procoduro and
insufficiont data of the progress of the
experiment to permit
an independent evaluation.
however, it is concluded that the Schwaitzor and
Arenco,
processes both yield IZTS wAth a rediiecd
biological potontial
than the stnrting tobacco blezidg that there J.#3
no dArtpronce
betwoun thage lhio RTS and that the
Taiuats-lftiuxii procO.133 yiOlds
an RTSt the condensate of' which possesses a
h:Lghor tuniorigonlaty
than either that of the starting tobacco or those
of the other
two RTS materials.
The findiiig,:3 with respect to the Schweit7.er
nnd Arenco
(Gerlach) proceeses are broadly in line with those
reported
oarlior from Lokstedt bv Lontonwill. The
Tamag-Eauni study
is the first bioloGical comparison for this
material of which
we are aviare.
IncidFntnl.lvl it is of interest to note in
passing that
this e~.pcrimzuntal sorics shows the "high dose
anomaly" in
cut.u-non. with inany othor mouso-skin paintinC
experiments.
With Und regnrd5,
Yours 01 C -Cly,
D.C. F, ~rO"N (PTO)
CD
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November 1999


cco: Dr. S.J. Green
Mr. G.C. Hargrove
Dr. F. llaslaml/
Encs.
cr,
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BATCo document for Province of British Columbia 9
November 1999


C 0
A jonc?-"-!ri'3 (100 dc-i-ii-,irw a~!s
curejuc'Cot! wit".1 unice,
itz puyjl.~;~~a bein-.1 -:,:c, ch-ack -L,'C-
U~i'j" ii" C~':..;r_
OL ~_C
ette s:;i~Azc ccnacn;~(;-Za u--;;,.-.
cniweit-zez foil, To;]. anc! %rcnco UZ,
t I
vuCh ~raz~ko froni a ctandaid
I 'C. ~-O~; CopicI1i '. C.". .,j r-c;l ic cil twol
L? i~'; :.z./L. I --_'6
L
-to tlia -~ollcwinui xc:.-u" `z:
'0 uz;*: Y c;.
vc,:-d in '61he test.
2.) Thic CC.-.1_-'C;:'_.-1-'-C qt:ctn
rric'.!-: uzdnz; f.)5-11 clid fc-ii,
C2 Of '6FOH.
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BATCo document for Province of British Columbia 9
November 1999


AM-=NCO**-. 2
2. ond prczonta-~Ion of.
Tho purpose of this 1cnzj--"-ozn eaubing tcrt to
arccr!.-cln `.--he
CCzCi.IICj,z;,Cn_;C C!",.Cct- of t-hc toz
obt-ain--d 'r'm C;~,rtain fo"I
et'..C3 Q3 comparce. to th.0 tor obt~ainad from a
ci~,Crctte.
Aftcr a period of, 1.1. mcnthz, Inttituto '1r,-r
CCncar RCzco_-..c!-.
ct thc University of Vicrina this test to the
foz Goncral. cild Pc-~holo y.
3. Hrtc;:_;cl3 under 'Ces".
C-riarct-tcs woz_- to pr;;.-.'uce tar
.011
"-. ;.) C r- y C
foil c*,.:,cr:~-.,;`c,
C.-I C U0 11 C:.
Smu::-. E::Pozt, UZ;CC., -.,70r
.:~-tio- of t'...c U:;,~;_'.
w:,,ra mc!:.';a of `6-,-.C sc;-.-,~ to!;c.- I
MI;";uro~; a,.
-he :;-.L;ndL:zd `u~-
PZP3~-- Vic:; .I1 z:.-.!
%.!a:; via:; r,,:.i anc
fo;.Lt- mo--, c w,
_1 (,(:,.:C,!.
wc;3
pac!,,.~.-., in t-*n'.:.'4' U.N7101-C
L/4
cr,
---I
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BATCo document for Province of British Columbia 9
November 1999


`C
2. of C-!, 0 C G 112 !:11C..
I Tho waic accurdi-m-1 -Lo ct-ar, U Q!1
Cu`_-,x-.!.-.i-'1-iC zmo!dni: aloc".incz. Thc waz,
in CC,ACzs
cn~.' (I
The conc'za~;u-'6-e wc:!, -Frora coolc_-*
vz--_!n~; a.CC`1:111i1C.
ThiG solv-don wc.-s avapozQtcd in a rotory
cvapt:,~Ca`-or
40 nm cf.rezcuxy. The fincl pro6uct c. dry
CC-,-2on:-
a,rc - VG,; dil-ji:cC,~ ait!,. oci~L~;nc to 75%
und, 50%.
3.3. calljun~;Ci~o teo-'s
3.3.1. In ordcz `1-o "he uni"'o.-mi"y oic
n,~chuniccl ~.,mo';:-
nj LWO unwunt I.).' tor frt-a
cind CO:~1:1orcd. Four C! 171-in""I
nicu'.imn cnd wo-Lor c:uun'L_:- Lict; .t,;zc
Q:.~ %U!~trio (.,n '%u!~*.-:c_:1,.~n co:-v),:ny.
Fuxthcxriore, ecc!i ProcAlct wc:; c-icr, -~or
~npi-!-:.nol
and b:~nzwp*'Irarlc.
1,n-;,rC`i _~'IV~l Cl*~; 'Ll'iC- L.'0-
of
B x
i42 247
14 Z 264
1 ; he nol !Ili,-. 58 92) 1185 1.59
YC
7
pq/cj.-
.:.0. 01.9 0.056 0 . OM 0.041
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BATCo document for Province of BritiSh Columbia 9
November 1999


Q
AREP CO***
3.3.3.
Cont'invove mr!~'zi: -of fz~z
(75 m910.1 m!).
Compound
s X
Hlcotinc 1.72 2.4-2 3.23
9.9 ~3 . 1 14.6 6.4
Tm in tht: dry
pcr 'est ~U.
P 1~1 z C 2.
G up A 0.4444 0.01431
Gruup D 0. 93. 1. 1 0.03,D37
Group S 0.92~;7 g 0. 03095
Group X 0. 75i*...5 0.01252
:are c, v-
1 Uh -.10 F.': CC, b 10 -1:; -'-u'7
T C' C:.*l C e
.-'~Ozca-rch C!*.- fr*-r cj"7:z
4- 10
C t t:'. Z'-" rr.11n.,- '.!c2t, 15
J.. 0 C n 3 v i 1cl I G 1: 1 c'! I
0 L: L Z! 3.- Gc nc., I c; z 11:;c
U1
BATCo document for Province of BritiSh Columbia 9
November 1999


Clc.-s--- at on
J. 7 3, %ni.-:,als
0 7 n 1 ni L, 1 s
i C "nimals
11.1 G I
F T- .",nirao13
G 7~
H 7 j I f. 13
L 75 rufcZmcc c;-:'Up
To -
t*c!!.,pc-,--o'--;j-.-e
C-:0 -r-com ".3 'U'l., (.4
22-5
4.2.2.
Tha ,:I.-Ct. in racieroloo cc-.,:cz in ~)rcup: oF
Mtrcml:.-i R" Mtrcirdn C~rnb"
"LaSe, p p e Z e)o ti,
(3 x,#C.n aci Uxbltum.
U1
U-I
0-
c::)
BATCo document for Province of BritiSh Columbia 9
November 1999


AMEA-'CO***
4.3 . Txectmen-'..- o-F -.~hc tcl-.- anina.'--
YCC.' durO4-ion
NO wcc!zt;
4. 3. 2. The bccl,:-.-; of c1l mico veze c'--i!-,-
--,,j :,rc,: evc V 0
usinj cn Acsculcp 'hui). clil'-p-cr.
3 . 1 On
'-az %/-,i:; cl")~.C:-! of
an~'rici' - `hrou ti.o~- cl
oil
O.C
Thc qua.""'i'l-," PL-Z o.: `Zcr
-3.3.2. Donc,-e a n c' t I c o f t- ( j r r C I C
0 0 '"1
75 i-.),j uz f ci,
U:.I C 0 1 L,
1 ; 50 A
C 75
50 B
-F C., 75 n t m: S
50 a 5
75 n 2: v X
50 C: i x
V -:.Ou - -.4 nc; a 1.- -:-j!-,c
L z L: :7 7: ;1
G-I
BATCo document for Province of British Columbia 9
November 1999


7
To enablz- th-_ cnimal:; to et uz-,-" 'l-C) t!)o
il-~Cctinc _'1l tha ttir,
wc~ekt of
t 0!; ". Duxin~ the toz;t 'Zhoa ta~r. 1`030
v;zi::; 1/3" 01' '4,11.0
qQun"ity givcn rabove cr.G' 6urine; t.~,-.e
Bcccpr.:.! tl-,ru doc;e
of tho fin(A ldo~~e.
C-F tczt cininc:15
On Z--
c! z o c: n tt~ rn t iz
1. 2. z;u c L, c
%.ms c,.wAr.:::6 rin-i
an-/ oy
-,u V iv 11 - on i.-Ic N, o U t a-'- U ")o
of
z 46
ldca "w"
v 17 \' L vr. '1;1.aI
CA
ho, b,- C)
k "".;:C
0 _1
BATCo document for Province of British Columbia 9
November 1999


(;:I-,! CjiVCl C1*11'G-,."-.,-,'OjiCa1
Oxc:
0 ;
".111 cutcnaouz c-nd zu'ocut-criaou.3 -.U.1;OUZ~;
Cn trec;,cd cr-
t;:cc.,'.cd --!z:,.n oncc mc~ze no`cz~ cn-':~zod
c;r.
foZ.--us vc; poz C.);., a;-,Y
rc, J-'j %/Zjzi a::
cc --tai; I a:--' b/ a 0 cz-- :.3 1-, il tU.M 0
UZ'5
all Oic COU 1C., .m:=0 z cc,.~; 1, Cc-..-,L C.;- n
r~j c-;:!a
f.:):;2j in -10/. ct-uoouz solution wi-Lh and
v -F C i 3; '. t 10 C, F a t 1 C a Z~ t 1 C, C: z
Z C
LO.-.la 07* ti
C
of Ull-L"Zou".06,
woze in 0 r a
icc- -13 %--zc- C! c:: Ow S
:1 c
Evaluction o -F tes-L rosul-I's
T 1, i C S U 1 L r. 0b 0 n J in t'l,:!
ro*-
C.11 il, C.*-.*U,_
Tvorv ".1c (17 c..'d
hi
-i-~ nviLcz o'F cn-!Mi1:,., 1))
pc~pilAowa, papi.\Aora, pcemoceroais ar
i cc;7c r.-nnic. c:-,-.!.; C)' SCcirCCmaS,
AovR -:nto
F
term
On of ti-,U s C: J. C. wg-c~::
Ul
BATCo document for Province of British Columbia 9
November 1999


9
5.3. Af-~ez '0, '00 cnd 100 wookr. tho r~!,-in
-"-ut.,.o-j3:c cn.; "ic sub-
cu-'Lluncous scrcomas Oi 'w.".0z~xe tiI2 was
-LzCq_tC(! vlzre
summod up in tabular for;,.l -For bot-h ilvinz;
cn~ aninc:13.
Ve.,:2 too, cnimaiz ldllcu at
crc incliudcd th3:o li'llin"; 3.1) 'cW,, 100`1-h-
Crily. un-
--lomuz fj,,cn-
OCIu2.'/0^_a_, ptipil- und cazcino-,,,icas
dl-,:~~;i,,-nated us skin the first- "bor-InnIn-
lom=-" cnd wera no-'- ~_,3kon
~~o C:C; not to Z-Z~sullts. into con-
cni.y if U:;POU::Cd tho C;:Cc. Lou-
kc.:i:.;_-a mid' GcCuI:2:::j on untzcc-~C~.,'
T_~C'CjUCIICY CZ; CW1 animals. This ra,.~cn-,
-'Lhut
thez;~, dcacauc,_ should bo cun::idurcd nul'u:01
-"-u md-ce, anj
sir;ni-.~'icant with xe~;r,;:c; -'~.-c,
"ccmc-rcr~crdc cHect- 61' 'to!,accc
to,". 7:1 the columm Lcti?ed "T.:Ptcil nu.-,!)c.,:
o4: 4-v.,~ou-,_."
and for abrwc-
monzionod
mct-ho-is
Th.- --.:-,n toz-, uZc-c'. in Grc'cr to cl-.Cck
UA
ibution C" :i-zi n tum- -'r3 1'. C'! -0 t 75
T n c---,!--r t;~) c*,-.cc!c t:.-.2
0 t Ou, r r: -L
.2
cnt'. 0':
X
A, 13 S t~e .,tanclox-A
U
Yhe C~f
FcxpI*iAet,%cxs, pre-
caricero,,es, carc.~ncnas, soxcom.,cs, XclAkaemci
arO hist.1ccy+0nac,
BATCO document for Province of BritiSh Columbia 9
November 1999


-A R-ENICL-3
j-,
fr~;.n 75 m~ d*c:c':z;c t-otalled 44. T!.-,z::c
doz,-,~;cz %!1.13. couzo at lco.-,- on czju;~!
that b~o~
OV "X=Vra. lowc~r ec,3::;o to cauz.- thin--ourz
'I-F thut 116 shou-16
be OC:UC11y t-:-.o `I-wo azzvps --:,- c.-cmin,:J
by ra:!ans of -LI.-o Z31---r. i-*.-' bc:cc-,ie3
cv.- -'-a.' 'h:
IOOSIS ccn "-,a zo-jcr--*-cd, -"-Fc cz--o;. b -
2 :
V."-:; L;--d to Out C!
6.5.2.
of -~,-Umovr .-C:toz ccu~;::U, by t-cr A, Ib cnc'
S
xC-.-,LCC cuu--ce 1;~ tcr 9. i:c-rc -'.co, of 'A;a
O-F tumourz; within diff-;~-.-cnt cirou-T)z cf-
llvinc
ardmclz c.-F-Zcr 100
D U d t I z 1) r; t 0 C c I C, I IC; F in -.4-esc
nrc C:~; nci, icr
s!iouli r.c,"- !i,.,va cn,, on ;:c-cu-I-*.---.
-1 0') o-Fr, C C L: 111
Tcr A B 5 x
10 Twj i.. nc: I z 9 33 9 2A
66 42 66 54
A 15 s x
6 23 6 9
nra "U.-;iOu- 69 52, 69 6.6
z 1 -.1 U.
c VC C. r,
;,as A oV wIhe'-'-.-z:: (.1os .!Qs
C13a
75 n~; o- 50 ml. o c c. t V 12
L31
cr,
BATCO document for Province of BritiSh Columbia 9
November 1999


-Ar-,'- CD*-k*
vic-Z er)-l-ozed in 3 3.2 ui.-
6.2. The of z:"_'ii v z~
40 90 100
n !n;-z-z
U3 :e CIVC-1 1-il A. T e r. i: c r r-,f - an co:.-
animcis in
In. 'Club-le~, A cnd B pa:ovides
numbey.-, in tublo C. The!;o rt.,proscn-'C oft
Ld-;.n
40 ~h, 80th
in
COZ: bo i v arx~ i~~A an-
1 CO"! vec'-:; of e " r. z t .
T a b !a II nCU. n 0 u. C C~ 1 C n C;." 17. r C!
-~O C s 1. CA C C 1.." C, 1 C: t-he
0 L.C~c 0-. '_incc a r 1.1;.:. CIT
'CWQLI':3 Ci-,"CU7~_0 C.1,; CLS oll
tcz b. T'his fcct- i~; "n
U. The :7c:--. c." tlti~ 'c-:
6 . L"- . L; x p x i r, in I - - z:
r Ck u I U o
1 -.1 C.I. I Z! Z U;.,. 0 U X i C; -L ic" I iL. 7
50 T
IGO
-rar A _TqT 6 TN r S To r X
50 mc
.3 33
71
755 23 6 9 4_1~
7 he tomokAns -fcco +he 5D m,3 dosa5c tnic\A.IetI
72. 16Z ~~=curs c:::>
BATCO document for Province of BritiSh Columbia 9
November 1999


6 S t- c: t- z c c I c!-. --, c!-, o t h a - u m
c~ r x a -t -ic r tv. z A 5
as X:
To:- AIS x
50 r,,-,: I-ximalz 9 21
..nimclz -ult-h.out -Lumours- 66 54
At. on=- dcr:--co o-7 -*--'.,i:; pzovidan un
Xzvclue of 6.0,110
and ic; thuz z5--n. c-6-. the 2.%o 'Lev,-!.
Tcx A/$ x
75 -9
i,.nimalz t-n-tuZz 0 66
For th-2 75 m3 -tar X.- unz, 0.02S6 w:,Icl-, no
at this CO.-*,C;Zn!:-,,C:,~,-,O,,1.
6.5.5. of for -Lci--- 6
"Cc, -
v !5-4
4 Z
2
X u-F 4.3.66 cn,.;
~-,;GL U`
Tor
75 23. 9
V.-I 0 LI r 52
66
C.-Ce. *.:~-
'i wn
-c!;
-X --z 7
, .
-
-
wo, -I
0 X C~G can---
CV:.1 C j Z!
50
A 5
CY,
BATCo document for Province of British Columbia 9
November 1999


Of 75 Ino Oil,
an orlor of 22.5%. A*- a dosage-
tor is n:? in i.-lic cipporancL, U-i
for
The ii 3: rcj to -.7oz u-7 C;z za~ II w -~y-poo
i
I j:, cc I\1 C Un C C: L O(J'Li" CO.-.Cc V -,I)
;-Or a
50 7
U ~;c 0 Orro,
75 mg i iz c z,:, p L, b ab.; 1. 1 -*.;>, w L:!;
6.5.7. T a i c1 `6- i c: c r: C C. I CA C I C C:
0 :1
C C;-.~; C 1~0:;c cuinz~-- lozz: -,mc,";
75 rr. ccnccn` ::-`,::n
G-F
'C-Z C:
z fz;,r c:; z:
01
CF
BATCo document for Province of British Columbia 9
November 1999