BA.T (U.K. & Export) Ltd.,
Research & Development Centre,
SOUTHAMPTON.
RLP/BTM
PROJECT ADDNOX: THE IDENTIFICATION
OFSMOKENOXAE PRECURSORS BY ADDING
SOLUBLE TOBACCO COMPONENTS TO
EXTRACTED TOBACCO BLENDS
REPORT NO: RD.2156
'?"Uecember 1989
AUTHOR: R.L. Prowse
SECTION LEADER: W.D.E. irwin
ISSUED BY: R.R. Baker
DISTRIBUTION:
Dr. R. Binns
Mr. A.L. Heard 2
Dr. J.S. Wqand 3
Dr. J.S. Wigand 4
Dr. P.J. Dunn 5
Dr. S.R. Massey 6
Mr. T.I. Wilson 7
Mr. T.I. Wilson 8
Dr. E. Rittershaus 9
Dr. E. Kausch 10
Dr. C.J.P. do Slqueira 11
Mr. H.V. Thomsen 12
Mr. E. Grant 13
Library 14
Library 15
Dr. R.E. Thornton 16
FILE: 46D
COPY NO.
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BATCo document for Province of British Columbia 28
October 1999


B-A.T (U.K. & Export) Ltd.,
Research & Development Centre,
SOUTHAMPTON.
RLP/BTM/46D
7 December 1989
PROJECT ADDNOX: THE IDENTIFICATION
OF SMOKE NOXAE PRECURSORS BY ADDING
SOLUBLE TOBACCO COMPONENTS TO
EXTRACTED TOBACCO BLENDS
REPORT NO. RD.2156
SUMMARY
This report describes studies aimed at identifying
those tobacco components
which are significant precursors of the 'Other
Noxae' as defined by the BAT
Scientific Research Group. The approach adopted
was to quantify the effects
on Other Noxae of adding soluble tobacco
components (sucrose, glucose,
fructose, rutin, protein hydrol~ysate and
potassium nitrate) to water-extracted
flue-cured or'Burley blends as appropriate. DEER
reconstftutlon of all products
was chosen to aftempt to minimise cigarette
variability.
In order to assist sample preparation, an
extraction system capable of handling
10 kg batches of tobacco at controlled
temperatures from ambient up to 90"C
(and which can also be used for enzyme studies)
was designed and built and is
described in this report.
The study indicates that in the context of a
burning cigarette, glucose, but not
sucrose or fructose is a catechol precursor. Of
these three sugars, only fructose
appears to yield acrolein. Rutin, as well as being
a significant catechol precursor,
was found to give rise to substantial yields of
acetaldehyde. Protein hydrotysate,
a precursor of smoke ammonia, appears to inhibit
the formation of formaldehyde
and acrolein perhaps by the formation of ammonia :
aldehyda adducts, although
the possibility of such reactions occurring during
smoke trapping remains a
possibility.
However, taken overall, the results confirm that
many noxae have such a variety
of sources in tobacco that significant reduction
by the targetting of precursors
for removal is likely to be complex and difficult,
particularly for those noxae which
do not contain nitrogen. Future studies will
therefore be aimed more at
investigating the effects of novel tobacco
processes on Other Noxae and of
addftives, etc., which may control the conversion
of precursors to Other Noxae.
Therefore, there will be less emphasis on, and
expectation of, specific noxae Xt.
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0 1989 BATUKE. Do not copy or show lo unauthorised
persons.
BATCo document for Province of British Columbia 28
October 1999


INDEXTERMS:
Toba Extraction
Tobacco Chemical Treatment
Tobacco Additives
Sucrose
Glucose
Fructose
Ruhn
Amino Acids
Nitrate
Potassium
ExtnxJed RTS
Cigarette Deliveries
Mainstream Smoke
Particulate Phase
Vapour Phase
Smoke Noxae
CD
CD
a 1989 BATUKE. Do not copy or show to unauthorlsed
persons.
BATCo document for Province of British Columbia 28
October 1999