RESEARCH DEPARTMENT
Analytical Report
No. A R 89-10-030
Subject AMES TEST ON GREENDOT SAMPLES
GD6GD12
Philip Morris Extra Lights
Benson & Hedges Ultra Mild
Authors E.D. Massey
TO Mr. 1.C. Brown
Dr. P.C. Bevan
Circulation Dr. R.R. Baker
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EDM/DTM/46D
19 October 1989
AMES TEST ON GnEENDOT SAMPLES
GD 6 Gl) 12
Philip Morris Extra Lights
Benson & Hedges Ultra Mild
ANALYTICAL REPOFIT NO. AR-89-10-030
Author: E.D. Massey
Blends and Ciaaretles
GQ6 %
Blend Composition: WTS 25
Burley Tobacco (13 1 B) 25
Flue-cured (H2) 25
Oriental CAB, YAB S1/4 10
Flue-cured Y1 15
GO 12
Blend Composition: WTS 20
Flue-cured Y1 40
Burley B1 B 40
Both blends had an initial addition of 1 %
magnesium chloride and 10% glycerol before
being manufactured into cigarettes.
Philip Morris Extra Lights (PMEL) and Benson &
Hedges Ultra Milds (BHUM) were
obtained from outside sources.
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persons.
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2
* 279 flue-cured reference cigarette.
* 283 US-style reference cigarette.
Methodology
As per report AR-9-0 i - 119. Date of testing
17.2. 1989. Before sending tile condensates
to Toxicol Laboratories they were coded as
follows:
BATUKE R&D Toxicol Code
Code Sample 1 Sample 2
GO 6 89-19 89-20
GO 12 89-25 89-26
PMEL 89-21 89-22
BHUM 89-23 89-24
A 279 89-27
A 283 89-28
The returned Toxicol data is dated 20.2.1989 and
is stored uiider experiment number
B 127. Ames mutagenic ratios were calculated as
described in (1).
Results
The means of the data are plotted in Figure I and
summarised numerically in Table 1.
Consistent with previous data the specific
activity of A 279 was less than that of A 263.
The specific activity of PIVIEL, MILIM and A 283
were of a similar magnitude. GD 12
condensate was next lowest in activity with GD 6
of lower activity than GO, 12.
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October 1999


TABLE 1
AMES MUTAGENIC RATIOS
Specific Activity Activity Per Cigarette
Dosage Basis TPM PMWNF PMWNGF TPM PMWNF PMWNGF
Cigarette
A 283 1.0* 1.0*
A 279 0.49 0.51
GD6 0.54 0.58
GD12 0.65 0.72
PIVIEL 1.01 1.02
BHUM 0.93 0.92
*independently assigned as value of 1.
REFERENCE
1.01 11.33 10.20 9.74
0.50 5.30 4.80 4.60
0.69 3.58 3.19 3.06
0.85 3.43 3.12 2.97
1.03 6.38 5.74 5.47
0.95 5.38 4.84 4.62
1. BAT Report No. RD.2130,11.11.1988.
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13 ATCo document for Province of British Columbia
28 October 1999