RESEARCH DEPARTMENT
Analytical Report
No. AR 89-10-031
Subject AMES TEST ON GREENDOT SAMPLES
WTS 1, WTS 2, WTS 4 AND CRS 3
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B.A:I'l LI.K. ind Export) Limited
RESEAn & DEVELOPMENT CENTRE
c;(CHJr11A~tr1oN F.U61AND
Authors E.D. Massey
TO Mr. 1.G. Brown
Dr. C.C. Greig
Circulation Dr. R.R. Baker
File 46D Date- 19 October 1989
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for Manager
CONFIDENTIAL NO'T TO 1111 COP11 1) ()R S1 JOWN
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BATCo document for Province of British Columbia 28
October 1999


EDM/BTW46D
19 October 1989
AMES TEST ON GREENDOT SAMPLES
WTS 1, WTS 2, WTS 4 AND CRS 3
ANALYTICAL REPORT NO. AR-89-10-031
Author: E.D. Massey
Tobaccos and Cluarettes
WIS 1
Water treated stem samples were milled and
maintained in water at 1 00*C for 1 1/2
hours. The sample was then homogenised and cast as
a sheet material. The sheet
material was then cut and manufactured into
cigarettes.
QRS 3
Cut rolled stern was treated as for the WTS I
sample.
WTS 2
The water treated stem sample was treated as for
WTS I up to the stage of
homogenisation. However, after homogenising the
liquid phase was separated from
the solids and the liquid discarded. Further
amounts of water was then added to the
solid material, the sample homogenised cast as a
sheet then cut and manufactured
into a cigarette.
VVTS 4
4 t:i-
Water treated stem samples were pre extracted with
water which was discarded and
the sample then treated as for WTS 2.
00
0 1989 BATUKE. Do not copy or show to unauthorised
persons.
BATCO document for Province of BritiSh Columbia 28
October 1999


Methodology
AsperreportAR-9-Oi-119. Date of testing 28.9.1989.
Before sending the condensates
to Toxicol Laboratories they were coded as
follows:
BATUKE R&D
Code
WTS 1
WTS 2
WTS 4
WTS 3
Toxicol Code
Sample 1 Sample 2
89-94 89-100
89-95 89-101
89-97 89-102
89-96 89-103
A 279
A 283
89-98
89-99
The returned Toxicol data Is dated 2.10.1989 and
is stored under experiment number
B 146.
Results
The means of the data from duplicate experiments
is plotted in Figure 1. Thecondensate
from cigarettes A 283 has greater specific
activity than that from A 279 and so conforms
with the historical ranking of these two products.
Over the dose range tested, the
condensate from WTS 1 and CRS 3 were of equal
specific activity and similar to that
from A 279. Samples WTS 2 and WTS 4 were of lower
specific activity than WTS 1
and CRS 3.
a 1989 BATUKE. Do not copy or show to unaulborised
persons.
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BATCO document for Province of BritiSh Columbia 28
October 1999


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BATCo document for Province of British Columbia 28
October 1999